TGFβ/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands
Signalling by TGFβ superfamily factors plays an important role in tissue growth and cell proliferation. In Drosophila, the activity of the TGFβ/Activin signalling branch has been linked to the regulation of cell growth and proliferation, but the cellular and molecular basis for these functions are n...
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doaj-b95729427d3f4fc2a23124c9223905d62020-11-25T04:00:23ZengThe Royal SocietyOpen Biology2046-24412017-01-017110.1098/rsob.160258160258TGFβ/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glandsTorcato MartinsNadia EusebioAndreia CorreiaJoana MarinhoFernando CasaresPaulo S. PereiraSignalling by TGFβ superfamily factors plays an important role in tissue growth and cell proliferation. In Drosophila, the activity of the TGFβ/Activin signalling branch has been linked to the regulation of cell growth and proliferation, but the cellular and molecular basis for these functions are not fully understood. In this study, we show that both the RII receptor Punt (Put) and the R-Smad Smad2 are strongly required for cell and tissue growth. Knocking down the expression of Put or Smad2 in salivary glands causes alterations in nucleolar structure and functions. Cells with decreased TGFβ/Activin signalling accumulate intermediate pre-rRNA transcripts containing internal transcribed spacer 1 regions accompanied by the nucleolar retention of ribosomal proteins. Thus, our results show that TGFβ/Activin signalling is required for ribosomal biogenesis, a key aspect of cellular growth control. Importantly, overexpression of Put enhanced cell growth induced by Drosophila Myc, a well-characterized inducer of nucleolar hypertrophy and ribosome biogenesis.https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.160258drosophilacell growthpunt (put)tgfβ/activinnucleolusribosome |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Torcato Martins Nadia Eusebio Andreia Correia Joana Marinho Fernando Casares Paulo S. Pereira |
spellingShingle |
Torcato Martins Nadia Eusebio Andreia Correia Joana Marinho Fernando Casares Paulo S. Pereira TGFβ/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands Open Biology drosophila cell growth punt (put) tgfβ/activin nucleolus ribosome |
author_facet |
Torcato Martins Nadia Eusebio Andreia Correia Joana Marinho Fernando Casares Paulo S. Pereira |
author_sort |
Torcato Martins |
title |
TGFβ/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands |
title_short |
TGFβ/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands |
title_full |
TGFβ/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands |
title_fullStr |
TGFβ/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands |
title_full_unstemmed |
TGFβ/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands |
title_sort |
tgfβ/activin signalling is required for ribosome biogenesis and cell growth in drosophila salivary glands |
publisher |
The Royal Society |
series |
Open Biology |
issn |
2046-2441 |
publishDate |
2017-01-01 |
description |
Signalling by TGFβ superfamily factors plays an important role in tissue growth and cell proliferation. In Drosophila, the activity of the TGFβ/Activin signalling branch has been linked to the regulation of cell growth and proliferation, but the cellular and molecular basis for these functions are not fully understood. In this study, we show that both the RII receptor Punt (Put) and the R-Smad Smad2 are strongly required for cell and tissue growth. Knocking down the expression of Put or Smad2 in salivary glands causes alterations in nucleolar structure and functions. Cells with decreased TGFβ/Activin signalling accumulate intermediate pre-rRNA transcripts containing internal transcribed spacer 1 regions accompanied by the nucleolar retention of ribosomal proteins. Thus, our results show that TGFβ/Activin signalling is required for ribosomal biogenesis, a key aspect of cellular growth control. Importantly, overexpression of Put enhanced cell growth induced by Drosophila Myc, a well-characterized inducer of nucleolar hypertrophy and ribosome biogenesis. |
topic |
drosophila cell growth punt (put) tgfβ/activin nucleolus ribosome |
url |
https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.160258 |
work_keys_str_mv |
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