H<sub>2</sub>O<sub>2</sub>-Based Method for Rapid Detection of Transgene-Free Rice Plants from Segregating CRISPR/Cas9 Genome-Edited Progenies

H<sub>2</sub>O<sub>2</sub>-Based Method for Rapid Detection of Transgene-Free Rice Plants from Segregating CRISPR/Cas9 Genome-Edited Progenies

Genome-editing techniques such as CRISPR/Cas9 have been widely used in crop functional genomics and improvement. To efficiently deliver the guide RNA and Cas9, most studies still rely on <i>Agrobacterium</i>-mediated transformation, which involves a selection marker gene. However, severa...

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Bibliographic Details
Main Authors: Tsung-Meng Wu, Jian-Zhi Huang, Hui-Min Oung, Yi-Ting Hsu, Yu-Chang Tsai, Chwan-Yang Hong
Format: Article
Language:English
Published: MDPI AG 2019-08-01
Series:International Journal of Molecular Sciences
Subjects:
DAB (3,3′-diaminobenzidine)
H<sub>2</sub>O<sub>2</sub>
hygromycin
hygromycin phosphotransferase
rice
CRISPR
reactive oxygen species
Online Access:https://www.mdpi.com/1422-0067/20/16/3885

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