Compartmental analyses of plasma n-3 essential fatty acids among male and female smokers and nonsmokers
The effects of cigarette smoking on n-3 essential FA metabolism were studied in male and female subjects by fitting the concentration-time curves of the d5-labeled plasma fatty acids (FAs) originating from a dose of d5-18:3n-3 to a compartmental model of n-3 FA metabolism. For 3 weeks, female (smoke...
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doaj-b95bcec2d7b045298fe87136811987572021-04-27T04:47:42ZengElsevierJournal of Lipid Research0022-22752007-04-01484935943Compartmental analyses of plasma n-3 essential fatty acids among male and female smokers and nonsmokersRobert J. Pawlosky0Joseph R. Hibbeln1Norman Salem2Laboratory of Metabolic Control, National Institutes on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MDLaboratory of Membrane Biochemistry and Biophysics, National Institutes on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MDLaboratory of Membrane Biochemistry and Biophysics, National Institutes on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MDThe effects of cigarette smoking on n-3 essential FA metabolism were studied in male and female subjects by fitting the concentration-time curves of the d5-labeled plasma fatty acids (FAs) originating from a dose of d5-18:3n-3 to a compartmental model of n-3 FA metabolism. For 3 weeks, female (smokers, n = 5; nonsmokers, n = 5) and male (smokers, n = 5; nonsmokers, n = 5) subjects subsisted on a beef-based diet. Beginning in the third week, subjects received a dose of d5-18:3n-3 ethyl ester (1 g). Plasma FAs were analyzed using gas chromatography (GC) and GC-mass spectrometry, and the kinetic rate parameters were determined from the concentration-time curves for d5-18:3n-3, d5-20:5n-3, d5-22:5n-3, and d5-22:6n-3. Women smokers had a 2-fold greater percent of dose in plasma (5.8% vs. 2.9%; P < 0.01) and a higher fractional rate constant coefficient for formation of d5-22:6n-3 from d5-22:5n-3 (0.03 h−1 vs. 0.01 h−1; P < 0.01), compared with nonsmokers. Male smokers had elevated total plasma n-3 FAs, more-rapid turnover of 18:3n-3 (13.3 mg/day−1 vs. 4.3 mg/day−1; P < 0.001), a disappearance rate of d5-20:5n-3 that was both delayed and slower (0.001 h−1 vs. 0.012 h−1; P < 0.05), and a percentage of d5-20:5n-3 directed into formation of d5-22:5n-3 (99% vs. 61%; P < 0.03) that was greater compared with nonsmokers. Smoking increased the bioavailability of n-3 FAs from plasma, accelerated the fractional synthetic rates, and heightened the percent formation of some long-chain n-3 PUFAs in men and women.http://www.sciencedirect.com/science/article/pii/S0022227520434467linolenic aciddocosahexaenoic acidisotope tracermass spectrometryhumanskinetics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Robert J. Pawlosky Joseph R. Hibbeln Norman Salem |
spellingShingle |
Robert J. Pawlosky Joseph R. Hibbeln Norman Salem Compartmental analyses of plasma n-3 essential fatty acids among male and female smokers and nonsmokers Journal of Lipid Research linolenic acid docosahexaenoic acid isotope tracer mass spectrometry humans kinetics |
author_facet |
Robert J. Pawlosky Joseph R. Hibbeln Norman Salem |
author_sort |
Robert J. Pawlosky |
title |
Compartmental analyses of plasma n-3 essential fatty acids among male and female smokers and nonsmokers |
title_short |
Compartmental analyses of plasma n-3 essential fatty acids among male and female smokers and nonsmokers |
title_full |
Compartmental analyses of plasma n-3 essential fatty acids among male and female smokers and nonsmokers |
title_fullStr |
Compartmental analyses of plasma n-3 essential fatty acids among male and female smokers and nonsmokers |
title_full_unstemmed |
Compartmental analyses of plasma n-3 essential fatty acids among male and female smokers and nonsmokers |
title_sort |
compartmental analyses of plasma n-3 essential fatty acids among male and female smokers and nonsmokers |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2007-04-01 |
description |
The effects of cigarette smoking on n-3 essential FA metabolism were studied in male and female subjects by fitting the concentration-time curves of the d5-labeled plasma fatty acids (FAs) originating from a dose of d5-18:3n-3 to a compartmental model of n-3 FA metabolism. For 3 weeks, female (smokers, n = 5; nonsmokers, n = 5) and male (smokers, n = 5; nonsmokers, n = 5) subjects subsisted on a beef-based diet. Beginning in the third week, subjects received a dose of d5-18:3n-3 ethyl ester (1 g). Plasma FAs were analyzed using gas chromatography (GC) and GC-mass spectrometry, and the kinetic rate parameters were determined from the concentration-time curves for d5-18:3n-3, d5-20:5n-3, d5-22:5n-3, and d5-22:6n-3. Women smokers had a 2-fold greater percent of dose in plasma (5.8% vs. 2.9%; P < 0.01) and a higher fractional rate constant coefficient for formation of d5-22:6n-3 from d5-22:5n-3 (0.03 h−1 vs. 0.01 h−1; P < 0.01), compared with nonsmokers. Male smokers had elevated total plasma n-3 FAs, more-rapid turnover of 18:3n-3 (13.3 mg/day−1 vs. 4.3 mg/day−1; P < 0.001), a disappearance rate of d5-20:5n-3 that was both delayed and slower (0.001 h−1 vs. 0.012 h−1; P < 0.05), and a percentage of d5-20:5n-3 directed into formation of d5-22:5n-3 (99% vs. 61%; P < 0.03) that was greater compared with nonsmokers. Smoking increased the bioavailability of n-3 FAs from plasma, accelerated the fractional synthetic rates, and heightened the percent formation of some long-chain n-3 PUFAs in men and women. |
topic |
linolenic acid docosahexaenoic acid isotope tracer mass spectrometry humans kinetics |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520434467 |
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