Identification and functional analysis of antifungal immune response genes in Drosophila.
Essential aspects of the innate immune response to microbial infection appear to be conserved between insects and mammals. Although signaling pathways that activate NF-kappaB during innate immune responses to various microorganisms have been studied in detail, regulatory mechanisms that control othe...
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2008-01-01
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Series: | PLoS Pathogens |
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doaj-b97ab7b764a4456093fe8fe4589e78c22020-11-24T21:20:03ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742008-01-01410e100016810.1371/journal.ppat.1000168Identification and functional analysis of antifungal immune response genes in Drosophila.Li Hua JinJaewon ShimJoon Sun YoonByungil KimJihyun KimJeongsil Kim-HaYoung-Joon KimEssential aspects of the innate immune response to microbial infection appear to be conserved between insects and mammals. Although signaling pathways that activate NF-kappaB during innate immune responses to various microorganisms have been studied in detail, regulatory mechanisms that control other immune responses to fungal infection require further investigation. To identify new Drosophila genes involved in antifungal immune responses, we selected genes known to be differentially regulated in SL2 cells by microbial cell wall components and tested their roles in antifungal defense using mutant flies. From 130 mutant lines, sixteen mutants exhibited increased sensitivity to fungal infection. Examination of their effects on defense against various types of bacteria and fungi revealed nine genes that are involved specifically in defense against fungal infection. All of these mutants displayed defects in phagocytosis or activation of antimicrobial peptide genes following infection. In some mutants, these immune deficiencies were attributed to defects in hemocyte development and differentiation, while other mutants showed specific defects in immune signaling required for humoral or cellular immune responses. Our results identify a new class of genes involved in antifungal immune responses in Drosophila.http://europepmc.org/articles/PMC2542415?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Li Hua Jin Jaewon Shim Joon Sun Yoon Byungil Kim Jihyun Kim Jeongsil Kim-Ha Young-Joon Kim |
spellingShingle |
Li Hua Jin Jaewon Shim Joon Sun Yoon Byungil Kim Jihyun Kim Jeongsil Kim-Ha Young-Joon Kim Identification and functional analysis of antifungal immune response genes in Drosophila. PLoS Pathogens |
author_facet |
Li Hua Jin Jaewon Shim Joon Sun Yoon Byungil Kim Jihyun Kim Jeongsil Kim-Ha Young-Joon Kim |
author_sort |
Li Hua Jin |
title |
Identification and functional analysis of antifungal immune response genes in Drosophila. |
title_short |
Identification and functional analysis of antifungal immune response genes in Drosophila. |
title_full |
Identification and functional analysis of antifungal immune response genes in Drosophila. |
title_fullStr |
Identification and functional analysis of antifungal immune response genes in Drosophila. |
title_full_unstemmed |
Identification and functional analysis of antifungal immune response genes in Drosophila. |
title_sort |
identification and functional analysis of antifungal immune response genes in drosophila. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Pathogens |
issn |
1553-7366 1553-7374 |
publishDate |
2008-01-01 |
description |
Essential aspects of the innate immune response to microbial infection appear to be conserved between insects and mammals. Although signaling pathways that activate NF-kappaB during innate immune responses to various microorganisms have been studied in detail, regulatory mechanisms that control other immune responses to fungal infection require further investigation. To identify new Drosophila genes involved in antifungal immune responses, we selected genes known to be differentially regulated in SL2 cells by microbial cell wall components and tested their roles in antifungal defense using mutant flies. From 130 mutant lines, sixteen mutants exhibited increased sensitivity to fungal infection. Examination of their effects on defense against various types of bacteria and fungi revealed nine genes that are involved specifically in defense against fungal infection. All of these mutants displayed defects in phagocytosis or activation of antimicrobial peptide genes following infection. In some mutants, these immune deficiencies were attributed to defects in hemocyte development and differentiation, while other mutants showed specific defects in immune signaling required for humoral or cellular immune responses. Our results identify a new class of genes involved in antifungal immune responses in Drosophila. |
url |
http://europepmc.org/articles/PMC2542415?pdf=render |
work_keys_str_mv |
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