COMPARISON BETWEEN FOUR USUAL METHODS OF IDENTIFICATION OF Candida SPECIES

• Main Authors: Margarida Neves SOUZA, Stéfanie Otowicz ORTIZ, Marcelo Martins MELLO, Flávio de Mattos OLIVEIRA, Luiz Carlos SEVERO, Cristine Souza GOEBEL
• Format: Article
• Language: English
• Published: Universidade de São Paulo 2015-08-01
• Series: Revista do Instituto de Medicina Tropical de São Paulo
• Subjects: Candida; Auxanogram; ID 32C; Chromogenic medium; Germ tube
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652015000400281&lng=en&tlng=en

SUMMARY Infection by Candidaspp. is associated with high mortality rates, especially when treatment is not appropriate and/or not immediate. Therefore, it is necessary to correctly identify the genus and species of Candida. The aim of this study was to compare the identification of 89 samples of Candida spp. by the manual methods germ tube test, auxanogram and chromogenic medium in relation to the ID 32C automated method. The concordances between the methods in ascending order, measured by the Kappa index were: ID 32C with CHROMagar Candida(κ = 0.38), ID 32C with auxanogram (κ = 0.59) and ID 32C with germ tube (κ = 0.9). One of the species identified in this study was C. tropicalis,which demonstrated a sensitivity of 46.2%, a specificity of 95.2%, PPV of 80%, NPV of 81.1%, and an accuracy of 80.9% in tests performed with CHROMagar Candida;and a sensitivity of 76.9%, a specificity of 96.8%, PPV of 90.9%, NPV of 91%, and an accuracy of 91% in the auxanogram tests. Therefore, it is necessary to know the advantages and limitations of methods to choose the best combination between them for a fast and correct identification of Candidaspecies.