Plasmablast, Memory B Cell, CD4+ T Cell, and Circulating Follicular Helper T Cell Responses to a Non-Replicating Modified Vaccinia Ankara Vaccine

Plasmablast, Memory B Cell, CD4+ T Cell, and Circulating Follicular Helper T Cell Responses to a Non-Replicating Modified Vaccinia Ankara Vaccine

Background<i>:</i> Vaccinia is known to induce antibody and cellular responses. Plasmablast, circulating follicular helper T (cT<sub>FH</sub>) cells, cytokine-expressing CD4 T cells, and memory B cells were compared between subcutaneous (SC) and needle-free jet injection (JI)...

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Main Authors: Evan J. Anderson, Lilin Lai, Jens Wrammert, Sarah Kabbani, Yongxian Xu, Lalita Priyamvada, Heather Hill, Johannes B. Goll, Travis L. Jensen, Carol Kao, Inci Yildirim, Nadine Rouphael, Lisa Jackson, Mark J. Mulligan
Format: Article
Language:English
Published: MDPI AG 2020-02-01
Series:Vaccines
Subjects:
mva
smallpox
follicular helper t cells (tfh), plasmablasts
vaccinia
antibody secreting cells
Online Access:https://www.mdpi.com/2076-393X/8/1/69
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spelling doaj-b981b0a028ff4964854270d33aae4b512020-11-25T02:06:05ZengMDPI AGVaccines2076-393X2020-02-01816910.3390/vaccines8010069vaccines8010069Plasmablast, Memory B Cell, CD4+ T Cell, and Circulating Follicular Helper T Cell Responses to a Non-Replicating Modified Vaccinia Ankara VaccineEvan J. Anderson0Lilin Lai1Jens Wrammert2Sarah Kabbani3Yongxian Xu4Lalita Priyamvada5Heather Hill6Johannes B. Goll7Travis L. Jensen8Carol Kao9Inci Yildirim10Nadine Rouphael11Lisa Jackson12Mark J. Mulligan13.Departments of Pediatrics and Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA.Hope Clinic, Department of Medicine, Emory University School of Medicine, Decatur, GA 30030, USA.Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA.Hope Clinic, Department of Medicine, Emory University School of Medicine, Decatur, GA 30030, USA.Hope Clinic, Department of Medicine, Emory University School of Medicine, Decatur, GA 30030, USA.Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA.The Emmes Corporation, Rockville, MD 20850, USA.The Emmes Corporation, Rockville, MD 20850, USA.The Emmes Corporation, Rockville, MD 20850, USA.Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA.Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA.Hope Clinic, Department of Medicine, Emory University School of Medicine, Decatur, GA 30030, USA.Kaiser Permanente, Seattle, WA 98101, USA.Hope Clinic, Department of Medicine, Emory University School of Medicine, Decatur, GA 30030, USABackground<i>:</i> Vaccinia is known to induce antibody and cellular responses. Plasmablast, circulating follicular helper T (cT<sub>FH</sub>) cells, cytokine-expressing CD4 T cells, and memory B cells were compared between subcutaneous (SC) and needle-free jet injection (JI) recipients of non-replicating modified vaccinia Ankara (MVA) vaccine. Methods<i>:</i> Vaccinia-na&#239;ve adults received MVA SC or by JI on Days 1 and 29. Vaccinia-specific antibodies were quantified by plaque reduction neutralization test (PRNT) and enzyme-linked immunosorbent assay. Plasmablast, cT<sub>FH</sub>, and cytokine-expressing CD4 T cells were assessed on Days 1, 8, 15, 29, 36, 43 (cT<sub>FH</sub> and CD4+ only) and 57. Memory B cells were measured on Days 1 and 57. Results<i>:</i> Of the 36 enrolled subjects, only 22 received both vaccinations and had evaluable specimens after the second vaccine. Plasmablasts peaked one week after each vaccine. Day 15 plasmablasts correlated with peak PRNT titers. cT<sub>FH</sub> peaked on Days 8 and 36 and correlated with Day 36 plasmablasts. CD4+ peaked at Day 29 and one-third produced &#8805;2 cytokines. Day 57 memory B cells ranged from 0.1% to 0.17% of IgG-secreting B cells. Conclusions<i>:</i> This study provides insights into the cellular responses to non-replicating MVA, currently used as a vector for a variety of novel vaccines.https://www.mdpi.com/2076-393X/8/1/69mvasmallpoxfollicular helper t cells (tfh), plasmablastsvacciniaantibody secreting cells
collection DOAJ
language English
format Article
sources DOAJ
author Evan J. Anderson
Lilin Lai
Jens Wrammert
Sarah Kabbani
Yongxian Xu
Lalita Priyamvada
Heather Hill
Johannes B. Goll
Travis L. Jensen
Carol Kao
Inci Yildirim
Nadine Rouphael
Lisa Jackson
Mark J. Mulligan
spellingShingle Evan J. Anderson
Lilin Lai
Jens Wrammert
Sarah Kabbani
Yongxian Xu
Lalita Priyamvada
Heather Hill
Johannes B. Goll
Travis L. Jensen
Carol Kao
Inci Yildirim
Nadine Rouphael
Lisa Jackson
Mark J. Mulligan
Plasmablast, Memory B Cell, CD4+ T Cell, and Circulating Follicular Helper T Cell Responses to a Non-Replicating Modified Vaccinia Ankara Vaccine
Vaccines
mva
smallpox
follicular helper t cells (tfh), plasmablasts
vaccinia
antibody secreting cells
author_facet Evan J. Anderson
Lilin Lai
Jens Wrammert
Sarah Kabbani
Yongxian Xu
Lalita Priyamvada
Heather Hill
Johannes B. Goll
Travis L. Jensen
Carol Kao
Inci Yildirim
Nadine Rouphael
Lisa Jackson
Mark J. Mulligan
author_sort Evan J. Anderson
title Plasmablast, Memory B Cell, CD4+ T Cell, and Circulating Follicular Helper T Cell Responses to a Non-Replicating Modified Vaccinia Ankara Vaccine
title_short Plasmablast, Memory B Cell, CD4+ T Cell, and Circulating Follicular Helper T Cell Responses to a Non-Replicating Modified Vaccinia Ankara Vaccine
title_full Plasmablast, Memory B Cell, CD4+ T Cell, and Circulating Follicular Helper T Cell Responses to a Non-Replicating Modified Vaccinia Ankara Vaccine
title_fullStr Plasmablast, Memory B Cell, CD4+ T Cell, and Circulating Follicular Helper T Cell Responses to a Non-Replicating Modified Vaccinia Ankara Vaccine
title_full_unstemmed Plasmablast, Memory B Cell, CD4+ T Cell, and Circulating Follicular Helper T Cell Responses to a Non-Replicating Modified Vaccinia Ankara Vaccine
title_sort plasmablast, memory b cell, cd4+ t cell, and circulating follicular helper t cell responses to a non-replicating modified vaccinia ankara vaccine
publisher MDPI AG
series Vaccines
issn 2076-393X
publishDate 2020-02-01
description Background<i>:</i> Vaccinia is known to induce antibody and cellular responses. Plasmablast, circulating follicular helper T (cT<sub>FH</sub>) cells, cytokine-expressing CD4 T cells, and memory B cells were compared between subcutaneous (SC) and needle-free jet injection (JI) recipients of non-replicating modified vaccinia Ankara (MVA) vaccine. Methods<i>:</i> Vaccinia-na&#239;ve adults received MVA SC or by JI on Days 1 and 29. Vaccinia-specific antibodies were quantified by plaque reduction neutralization test (PRNT) and enzyme-linked immunosorbent assay. Plasmablast, cT<sub>FH</sub>, and cytokine-expressing CD4 T cells were assessed on Days 1, 8, 15, 29, 36, 43 (cT<sub>FH</sub> and CD4+ only) and 57. Memory B cells were measured on Days 1 and 57. Results<i>:</i> Of the 36 enrolled subjects, only 22 received both vaccinations and had evaluable specimens after the second vaccine. Plasmablasts peaked one week after each vaccine. Day 15 plasmablasts correlated with peak PRNT titers. cT<sub>FH</sub> peaked on Days 8 and 36 and correlated with Day 36 plasmablasts. CD4+ peaked at Day 29 and one-third produced &#8805;2 cytokines. Day 57 memory B cells ranged from 0.1% to 0.17% of IgG-secreting B cells. Conclusions<i>:</i> This study provides insights into the cellular responses to non-replicating MVA, currently used as a vector for a variety of novel vaccines.
topic mva
smallpox
follicular helper t cells (tfh), plasmablasts
vaccinia
antibody secreting cells
url https://www.mdpi.com/2076-393X/8/1/69
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