Lactobacillus Plantarum CQPC05 Isolated from Pickled Vegetables Inhibits Constipation in Mice
This study mainly investigated the influences of Lactobacillus plantarum CQPC05 (CQPC05) isolated from pickled vegetables on mouse constipation caused by activated carbon water. We used 16S rDNA technology to identify the microorganism, and activated carbon to establish a mouse constipation model. A...
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doaj-b987436392884a9bbd700c42d96b6a682020-11-25T00:05:31ZengMDPI AGApplied Sciences2076-34172019-01-019115910.3390/app9010159app9010159Lactobacillus Plantarum CQPC05 Isolated from Pickled Vegetables Inhibits Constipation in MiceFang Li0Hong Zhou1Xianrong Zhou2Ruokun Yi3Jianfei Mu4Xin Zhao5Weiwei Liu6Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, ChinaChongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, ChinaChongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, ChinaChongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, ChinaChongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, ChinaChongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, ChinaSchool of Public Health and Management, Chongqing Medical University, Chongqing 400016, ChinaThis study mainly investigated the influences of Lactobacillus plantarum CQPC05 (CQPC05) isolated from pickled vegetables on mouse constipation caused by activated carbon water. We used 16S rDNA technology to identify the microorganism, and activated carbon to establish a mouse constipation model. After the mice received L. plantarum (109 CFU/mL) by gavage, small intestine tissue sections were collected. The serum indices and small intestine-related mRNA expression were obtained. A strain of L. plantarum was identified, and named CQPC05. The body weight and activated carbon progradation rate in the mice of the CQPC05 treatment groups were significantly higher than those in the control group, and the excretion time of the first tarry stool was earlier than that of the control group. The results of serum indices indicated that serum gastrin (Gas), endothelin (ET), and acetylcholinesterase (AchE) levels in the CQPC05 treatment groups were significantly higher than those in the control group, while the somatostatin (SS) level was significantly lower. Compared to the constipation control group, the result of q-PCR demonstrated that CQPC05 could up-regulate the mRNA expression of c-Kit (stem cell factor receptor), SCF (stem cell factor), and GDNF (glial cell-derived neurotrophic factor) genes, and down-regulate the expression of TRPV1 (transient receptor potential cation channel subfamily V member 1) and iNOS (inducible nitric oxide synthase). In conclusion, L. plantarum CQPC05 can effectively alleviate constipation, and has good probiotic potential and application value.http://www.mdpi.com/2076-3417/9/1/159Lactobacillus plantarumactivated carbonconstipationexpressionmRNA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fang Li Hong Zhou Xianrong Zhou Ruokun Yi Jianfei Mu Xin Zhao Weiwei Liu |
spellingShingle |
Fang Li Hong Zhou Xianrong Zhou Ruokun Yi Jianfei Mu Xin Zhao Weiwei Liu Lactobacillus Plantarum CQPC05 Isolated from Pickled Vegetables Inhibits Constipation in Mice Applied Sciences Lactobacillus plantarum activated carbon constipation expression mRNA |
author_facet |
Fang Li Hong Zhou Xianrong Zhou Ruokun Yi Jianfei Mu Xin Zhao Weiwei Liu |
author_sort |
Fang Li |
title |
Lactobacillus Plantarum CQPC05 Isolated from Pickled Vegetables Inhibits Constipation in Mice |
title_short |
Lactobacillus Plantarum CQPC05 Isolated from Pickled Vegetables Inhibits Constipation in Mice |
title_full |
Lactobacillus Plantarum CQPC05 Isolated from Pickled Vegetables Inhibits Constipation in Mice |
title_fullStr |
Lactobacillus Plantarum CQPC05 Isolated from Pickled Vegetables Inhibits Constipation in Mice |
title_full_unstemmed |
Lactobacillus Plantarum CQPC05 Isolated from Pickled Vegetables Inhibits Constipation in Mice |
title_sort |
lactobacillus plantarum cqpc05 isolated from pickled vegetables inhibits constipation in mice |
publisher |
MDPI AG |
series |
Applied Sciences |
issn |
2076-3417 |
publishDate |
2019-01-01 |
description |
This study mainly investigated the influences of Lactobacillus plantarum CQPC05 (CQPC05) isolated from pickled vegetables on mouse constipation caused by activated carbon water. We used 16S rDNA technology to identify the microorganism, and activated carbon to establish a mouse constipation model. After the mice received L. plantarum (109 CFU/mL) by gavage, small intestine tissue sections were collected. The serum indices and small intestine-related mRNA expression were obtained. A strain of L. plantarum was identified, and named CQPC05. The body weight and activated carbon progradation rate in the mice of the CQPC05 treatment groups were significantly higher than those in the control group, and the excretion time of the first tarry stool was earlier than that of the control group. The results of serum indices indicated that serum gastrin (Gas), endothelin (ET), and acetylcholinesterase (AchE) levels in the CQPC05 treatment groups were significantly higher than those in the control group, while the somatostatin (SS) level was significantly lower. Compared to the constipation control group, the result of q-PCR demonstrated that CQPC05 could up-regulate the mRNA expression of c-Kit (stem cell factor receptor), SCF (stem cell factor), and GDNF (glial cell-derived neurotrophic factor) genes, and down-regulate the expression of TRPV1 (transient receptor potential cation channel subfamily V member 1) and iNOS (inducible nitric oxide synthase). In conclusion, L. plantarum CQPC05 can effectively alleviate constipation, and has good probiotic potential and application value. |
topic |
Lactobacillus plantarum activated carbon constipation expression mRNA |
url |
http://www.mdpi.com/2076-3417/9/1/159 |
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