A low-cost electrochemical biosensor platform for C-reactive protein detection
Antibiotic resistance is a worldwide problem aggravated by the overuse of prophylactic antibiotic therapies and lack of real-time biosensing equipment to differentiate viral from bacterial infections at the Point of Care (POC), particularly in rural areas with limited access to clinical laboratory f...
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doaj-b98ed28f2ad74391b960a260111a6e892021-03-03T04:21:46ZengElsevierSensing and Bio-Sensing Research2214-18042021-02-0131100402A low-cost electrochemical biosensor platform for C-reactive protein detectionPedro Guillem0Rosa-Helena Bustos1Vivian Garzon2Andres Munoz3Graciela Juez4Evidence-Based Therapeutics Group, Clinical Pharmacology, Universidad de La Sabana, 140013 Chía, Colombia; Osiris&Bioaxis Group, Programa de Bioingeniería, Facultad de Ingeniería, Universidad El Bosque, 110221 Bogotá, ColombiaEvidence-Based Therapeutics Group, Clinical Pharmacology, Universidad de La Sabana, 140013 Chía, Colombia; Corresponding author.Evidence-Based Therapeutics Group, Clinical Pharmacology, Universidad de La Sabana, 140013 Chía, ColombiaEvidence-Based Therapeutics Group, Clinical Pharmacology, Universidad de La Sabana, 140013 Chía, ColombiaOsiris&Bioaxis Group, Programa de Bioingeniería, Facultad de Ingeniería, Universidad El Bosque, 110221 Bogotá, ColombiaAntibiotic resistance is a worldwide problem aggravated by the overuse of prophylactic antibiotic therapies and lack of real-time biosensing equipment to differentiate viral from bacterial infections at the Point of Care (POC), particularly in rural areas with limited access to clinical laboratory facilities. As recent studies reveal the potential of C-Reactive Protein (CRP) and other acute phase biomarkers to achieve early determination of etiology in acute febrile illness, novel biosensing equipment become a plausible approach in preventing the unnecessary prescription of antibiotics. A low-cost experimental platform was engineered to measure CRP concentrations in 50 μL samples of buffer and enriched plasma, using label-free antigenic probes, legacy electrochemical methods and open-source hardware. The prototype presents a portable, cost-effective device for use at the POC with a simplified user interface that can be used wirelessly by healthcare professionals using a mobile phone or laptop. Based on the measured CRP levels, the device suggests if the acute febrile episode is likely to be of bacterial etiology.http://www.sciencedirect.com/science/article/pii/S2214180421000076Electrochemical biosensorAntibiotic resistanceCRPPoint-of-care diagnostics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pedro Guillem Rosa-Helena Bustos Vivian Garzon Andres Munoz Graciela Juez |
spellingShingle |
Pedro Guillem Rosa-Helena Bustos Vivian Garzon Andres Munoz Graciela Juez A low-cost electrochemical biosensor platform for C-reactive protein detection Sensing and Bio-Sensing Research Electrochemical biosensor Antibiotic resistance CRP Point-of-care diagnostics |
author_facet |
Pedro Guillem Rosa-Helena Bustos Vivian Garzon Andres Munoz Graciela Juez |
author_sort |
Pedro Guillem |
title |
A low-cost electrochemical biosensor platform for C-reactive protein detection |
title_short |
A low-cost electrochemical biosensor platform for C-reactive protein detection |
title_full |
A low-cost electrochemical biosensor platform for C-reactive protein detection |
title_fullStr |
A low-cost electrochemical biosensor platform for C-reactive protein detection |
title_full_unstemmed |
A low-cost electrochemical biosensor platform for C-reactive protein detection |
title_sort |
low-cost electrochemical biosensor platform for c-reactive protein detection |
publisher |
Elsevier |
series |
Sensing and Bio-Sensing Research |
issn |
2214-1804 |
publishDate |
2021-02-01 |
description |
Antibiotic resistance is a worldwide problem aggravated by the overuse of prophylactic antibiotic therapies and lack of real-time biosensing equipment to differentiate viral from bacterial infections at the Point of Care (POC), particularly in rural areas with limited access to clinical laboratory facilities. As recent studies reveal the potential of C-Reactive Protein (CRP) and other acute phase biomarkers to achieve early determination of etiology in acute febrile illness, novel biosensing equipment become a plausible approach in preventing the unnecessary prescription of antibiotics. A low-cost experimental platform was engineered to measure CRP concentrations in 50 μL samples of buffer and enriched plasma, using label-free antigenic probes, legacy electrochemical methods and open-source hardware. The prototype presents a portable, cost-effective device for use at the POC with a simplified user interface that can be used wirelessly by healthcare professionals using a mobile phone or laptop. Based on the measured CRP levels, the device suggests if the acute febrile episode is likely to be of bacterial etiology. |
topic |
Electrochemical biosensor Antibiotic resistance CRP Point-of-care diagnostics |
url |
http://www.sciencedirect.com/science/article/pii/S2214180421000076 |
work_keys_str_mv |
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