Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry

Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry

<p>Abstract</p> <p>Recent advances in protein mass spectrometry (MS) have enabled determinations of hydrogen deuterium exchange (HDX) in large macromolecular complexes. HDX-MS became a valuable tool to follow protein folding, assembly and aggregation. The methodology has a wide ran...

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Main Authors: Tuma Roman, Suchanova Bohumila
Format: Article
Language:English
Published: BMC 2008-04-01
Series:Microbial Cell Factories
Online Access:http://www.microbialcellfactories.com/content/7/1/12
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spelling doaj-b9b531cb651d49cf8263273d0a5c1f552020-11-24T20:53:39ZengBMCMicrobial Cell Factories1475-28592008-04-01711210.1186/1475-2859-7-12Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometryTuma RomanSuchanova Bohumila<p>Abstract</p> <p>Recent advances in protein mass spectrometry (MS) have enabled determinations of hydrogen deuterium exchange (HDX) in large macromolecular complexes. HDX-MS became a valuable tool to follow protein folding, assembly and aggregation. The methodology has a wide range of applications in biotechnology ranging from quality control for over-expressed proteins and their complexes to screening of potential ligands and inhibitors. This review provides an introduction to protein folding and assembly followed by the principles of HDX and MS detection, and concludes with selected examples of applications that might be of interest to the biotechnology community.</p> http://www.microbialcellfactories.com/content/7/1/12
collection DOAJ
language English
format Article
sources DOAJ
author Tuma Roman
Suchanova Bohumila
spellingShingle Tuma Roman
Suchanova Bohumila
Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry
Microbial Cell Factories
author_facet Tuma Roman
Suchanova Bohumila
author_sort Tuma Roman
title Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry
title_short Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry
title_full Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry
title_fullStr Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry
title_full_unstemmed Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry
title_sort folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry
publisher BMC
series Microbial Cell Factories
issn 1475-2859
publishDate 2008-04-01
description <p>Abstract</p> <p>Recent advances in protein mass spectrometry (MS) have enabled determinations of hydrogen deuterium exchange (HDX) in large macromolecular complexes. HDX-MS became a valuable tool to follow protein folding, assembly and aggregation. The methodology has a wide range of applications in biotechnology ranging from quality control for over-expressed proteins and their complexes to screening of potential ligands and inhibitors. This review provides an introduction to protein folding and assembly followed by the principles of HDX and MS detection, and concludes with selected examples of applications that might be of interest to the biotechnology community.</p>
url http://www.microbialcellfactories.com/content/7/1/12
work_keys_str_mv AT tumaroman foldingandassemblyoflargemacromolecularcomplexesmonitoredbyhydrogendeuteriumexchangeandmassspectrometry
AT suchanovabohumila foldingandassemblyoflargemacromolecularcomplexesmonitoredbyhydrogendeuteriumexchangeandmassspectrometry
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