STAT6, PBX2, and PBRM1 Emerge as Predicted Regulators of 452 Differentially Expressed Genes Associated With Puberty in Brahman Heifers
The liver plays a central role in metabolism and produces important hormones. Hepatic estrogen receptors and the release of insulin-like growth factor 1 (IGF1) are critical links between liver function and the reproductive system. However, the role of liver in pubertal development is not fully under...
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doaj-b9e9c4cca6954eea9e58a8e1421311152020-11-24T22:24:42ZengFrontiers Media S.A.Frontiers in Genetics1664-80212018-03-01910.3389/fgene.2018.00087338964STAT6, PBX2, and PBRM1 Emerge as Predicted Regulators of 452 Differentially Expressed Genes Associated With Puberty in Brahman HeifersLoan T. Nguyen0Loan T. Nguyen1Antonio Reverter2Angela Cánovas3Bronwyn Venus4Stephen T. Anderson5Alma Islas-Trejo6Marina M. Dias7Natalie F. Crawford8Sigrid A. Lehnert9Juan F. Medrano10Milt G. Thomas11Stephen S. Moore12Marina R. S. Fortes13Marina R. S. Fortes14School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, QLD, AustraliaFaculty of Biotechnology, Vietnam National University of Agriculture, Hanoi, VietnamCSIRO Agriculture and Food, Queensland Bioscience Precinct, St. Lucia, QLD, AustraliaCentre for Genetic Improvement of Livestock, Department of Animal Biosciences, University of Guelph, Guelph, ON, CanadaQueensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD, AustraliaSchool of Biomedical Sciences, The University of Queensland, Brisbane, QLD, AustraliaDepartment of Animal Science, University of California, Davis, Davis, CA, United StatesDepartamento de Zootecnia, Faculdade de Ciências Agráìrias e Veterináìrias, Universidade Estadual Paulista Júlio de Mesquita Filho, São Paulo, BrazilDepartment of Animal Science, Colorado State University, Fort Collins, CO, United StatesCSIRO Agriculture and Food, Queensland Bioscience Precinct, St. Lucia, QLD, AustraliaDepartment of Animal Science, University of California, Davis, Davis, CA, United StatesDepartment of Animal Science, Colorado State University, Fort Collins, CO, United StatesQueensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD, AustraliaSchool of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, QLD, AustraliaQueensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD, AustraliaThe liver plays a central role in metabolism and produces important hormones. Hepatic estrogen receptors and the release of insulin-like growth factor 1 (IGF1) are critical links between liver function and the reproductive system. However, the role of liver in pubertal development is not fully understood. To explore this question, we applied transcriptomic analyses to liver samples of pre- and post-pubertal Brahman heifers and identified differentially expressed (DE) genes and genes encoding transcription factors (TFs). Differential expression of genes suggests potential biological mechanisms and pathways linking liver function to puberty. The analyses identified 452 DE genes and 82 TF with significant contribution to differential gene expression by using a regulatory impact factor metric. Brain-derived neurotrophic factor was observed as the most down-regulated gene (P = 0.003) in post-pubertal heifers and we propose this gene influences pubertal development in Brahman heifers. Additionally, co-expression network analysis provided evidence for three TF as key regulators of liver function during pubertal development: the signal transducer and activator of transcription 6, PBX homeobox 2, and polybromo 1. Pathway enrichment analysis identified transforming growth factor-beta and Wnt signaling pathways as significant annotation terms for the list of DE genes and TF in the co-expression network. Molecular information regarding genes and pathways described in this work are important to further our understanding of puberty onset in Brahman heifers.http://journal.frontiersin.org/article/10.3389/fgene.2018.00087/fullBos indicuspubertygene expressionRNA sequencinggene networkliver |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Loan T. Nguyen Loan T. Nguyen Antonio Reverter Angela Cánovas Bronwyn Venus Stephen T. Anderson Alma Islas-Trejo Marina M. Dias Natalie F. Crawford Sigrid A. Lehnert Juan F. Medrano Milt G. Thomas Stephen S. Moore Marina R. S. Fortes Marina R. S. Fortes |
spellingShingle |
Loan T. Nguyen Loan T. Nguyen Antonio Reverter Angela Cánovas Bronwyn Venus Stephen T. Anderson Alma Islas-Trejo Marina M. Dias Natalie F. Crawford Sigrid A. Lehnert Juan F. Medrano Milt G. Thomas Stephen S. Moore Marina R. S. Fortes Marina R. S. Fortes STAT6, PBX2, and PBRM1 Emerge as Predicted Regulators of 452 Differentially Expressed Genes Associated With Puberty in Brahman Heifers Frontiers in Genetics Bos indicus puberty gene expression RNA sequencing gene network liver |
author_facet |
Loan T. Nguyen Loan T. Nguyen Antonio Reverter Angela Cánovas Bronwyn Venus Stephen T. Anderson Alma Islas-Trejo Marina M. Dias Natalie F. Crawford Sigrid A. Lehnert Juan F. Medrano Milt G. Thomas Stephen S. Moore Marina R. S. Fortes Marina R. S. Fortes |
author_sort |
Loan T. Nguyen |
title |
STAT6, PBX2, and PBRM1 Emerge as Predicted Regulators of 452 Differentially Expressed Genes Associated With Puberty in Brahman Heifers |
title_short |
STAT6, PBX2, and PBRM1 Emerge as Predicted Regulators of 452 Differentially Expressed Genes Associated With Puberty in Brahman Heifers |
title_full |
STAT6, PBX2, and PBRM1 Emerge as Predicted Regulators of 452 Differentially Expressed Genes Associated With Puberty in Brahman Heifers |
title_fullStr |
STAT6, PBX2, and PBRM1 Emerge as Predicted Regulators of 452 Differentially Expressed Genes Associated With Puberty in Brahman Heifers |
title_full_unstemmed |
STAT6, PBX2, and PBRM1 Emerge as Predicted Regulators of 452 Differentially Expressed Genes Associated With Puberty in Brahman Heifers |
title_sort |
stat6, pbx2, and pbrm1 emerge as predicted regulators of 452 differentially expressed genes associated with puberty in brahman heifers |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Genetics |
issn |
1664-8021 |
publishDate |
2018-03-01 |
description |
The liver plays a central role in metabolism and produces important hormones. Hepatic estrogen receptors and the release of insulin-like growth factor 1 (IGF1) are critical links between liver function and the reproductive system. However, the role of liver in pubertal development is not fully understood. To explore this question, we applied transcriptomic analyses to liver samples of pre- and post-pubertal Brahman heifers and identified differentially expressed (DE) genes and genes encoding transcription factors (TFs). Differential expression of genes suggests potential biological mechanisms and pathways linking liver function to puberty. The analyses identified 452 DE genes and 82 TF with significant contribution to differential gene expression by using a regulatory impact factor metric. Brain-derived neurotrophic factor was observed as the most down-regulated gene (P = 0.003) in post-pubertal heifers and we propose this gene influences pubertal development in Brahman heifers. Additionally, co-expression network analysis provided evidence for three TF as key regulators of liver function during pubertal development: the signal transducer and activator of transcription 6, PBX homeobox 2, and polybromo 1. Pathway enrichment analysis identified transforming growth factor-beta and Wnt signaling pathways as significant annotation terms for the list of DE genes and TF in the co-expression network. Molecular information regarding genes and pathways described in this work are important to further our understanding of puberty onset in Brahman heifers. |
topic |
Bos indicus puberty gene expression RNA sequencing gene network liver |
url |
http://journal.frontiersin.org/article/10.3389/fgene.2018.00087/full |
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