A Chrysanthemum Heat Shock Protein Confers Tolerance to Abiotic Stress

A Chrysanthemum Heat Shock Protein Confers Tolerance to Abiotic Stress

Heat shock proteins are associated with protection against various abiotic stresses. Here, the isolation of a chrysanthemum cDNA belonging to the HSP70 family is reported. The cDNA, designated CgHSP70, encodes a 647-residue polypeptide, of estimated molecular mass 70.90 kDa and pI 5.12. A sub-cellul...

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Main Authors: Aiping Song, Xirong Zhu, Fadi Chen, Haishun Gao, Jiafu Jiang, Sumei Chen
Format: Article
Language:English
Published: MDPI AG 2014-03-01
Series:International Journal of Molecular Sciences
Subjects:
abiotic stress tolerance
heat shock protein
transformation
Online Access:http://www.mdpi.com/1422-0067/15/3/5063
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spelling doaj-ba017045a69644a2bf258ea404a334c22020-11-24T23:06:48ZengMDPI AGInternational Journal of Molecular Sciences1422-00672014-03-011535063507810.3390/ijms15035063ijms15035063A Chrysanthemum Heat Shock Protein Confers Tolerance to Abiotic StressAiping Song0Xirong Zhu1Fadi Chen2Haishun Gao3Jiafu Jiang4Sumei Chen5College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaHeat shock proteins are associated with protection against various abiotic stresses. Here, the isolation of a chrysanthemum cDNA belonging to the HSP70 family is reported. The cDNA, designated CgHSP70, encodes a 647-residue polypeptide, of estimated molecular mass 70.90 kDa and pI 5.12. A sub-cellular localization assay indicated that the cDNA product is deposited in the cytoplasm and nucleus. The performance of Arabidopsis thaliana plants constitutively expressing CgHSP70 demonstrated that the gene enhances tolerance to heat, drought and salinity. When CgHSP70 was stably over-expressed in chrysanthemum, the plants showed an increased peroxidase (POD) activity, higher proline content and inhibited malondialdehyde (MDA) content. After heat stress, drought or salinity the transgenic plants were better able to recover, demonstrating CgHSP70 positive effect.http://www.mdpi.com/1422-0067/15/3/5063abiotic stress toleranceheat shock proteintransformation
collection DOAJ
language English
format Article
sources DOAJ
author Aiping Song
Xirong Zhu
Fadi Chen
Haishun Gao
Jiafu Jiang
Sumei Chen
spellingShingle Aiping Song
Xirong Zhu
Fadi Chen
Haishun Gao
Jiafu Jiang
Sumei Chen
A Chrysanthemum Heat Shock Protein Confers Tolerance to Abiotic Stress
International Journal of Molecular Sciences
abiotic stress tolerance
heat shock protein
transformation
author_facet Aiping Song
Xirong Zhu
Fadi Chen
Haishun Gao
Jiafu Jiang
Sumei Chen
author_sort Aiping Song
title A Chrysanthemum Heat Shock Protein Confers Tolerance to Abiotic Stress
title_short A Chrysanthemum Heat Shock Protein Confers Tolerance to Abiotic Stress
title_full A Chrysanthemum Heat Shock Protein Confers Tolerance to Abiotic Stress
title_fullStr A Chrysanthemum Heat Shock Protein Confers Tolerance to Abiotic Stress
title_full_unstemmed A Chrysanthemum Heat Shock Protein Confers Tolerance to Abiotic Stress
title_sort chrysanthemum heat shock protein confers tolerance to abiotic stress
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2014-03-01
description Heat shock proteins are associated with protection against various abiotic stresses. Here, the isolation of a chrysanthemum cDNA belonging to the HSP70 family is reported. The cDNA, designated CgHSP70, encodes a 647-residue polypeptide, of estimated molecular mass 70.90 kDa and pI 5.12. A sub-cellular localization assay indicated that the cDNA product is deposited in the cytoplasm and nucleus. The performance of Arabidopsis thaliana plants constitutively expressing CgHSP70 demonstrated that the gene enhances tolerance to heat, drought and salinity. When CgHSP70 was stably over-expressed in chrysanthemum, the plants showed an increased peroxidase (POD) activity, higher proline content and inhibited malondialdehyde (MDA) content. After heat stress, drought or salinity the transgenic plants were better able to recover, demonstrating CgHSP70 positive effect.
topic abiotic stress tolerance
heat shock protein
transformation
url http://www.mdpi.com/1422-0067/15/3/5063
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