Direct visualization of a Fe(IV)–OH intermediate in a heme enzyme

The nature of the ferryl intermediate generated in reactions catalysed by heme-containing enzymes is uncertain, due to the ambiguity of X-ray crystallography data. Here, the authors apply neutron diffraction, kinetics and other spectroscopy to directly observe a protonated ferryl intermediate in a h...

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Main Authors:	Hanna Kwon, Jaswir Basran, Cecilia M. Casadei, Alistair J. Fielding, Tobias E. Schrader, Andreas Ostermann, Juliette M. Devos, Pierre Aller, Matthew P. Blakeley, Peter C. E. Moody, Emma L. Raven
Format:	Article
Language:	English
Published:	Nature Publishing Group 2016-11-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/ncomms13445

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spelling	doaj-ba0cf1877308498b991c38c8062c04312021-05-11T10:56:03ZengNature Publishing GroupNature Communications2041-17232016-11-01711610.1038/ncomms13445Direct visualization of a Fe(IV)–OH intermediate in a heme enzymeHanna Kwon0Jaswir Basran1Cecilia M. Casadei2Alistair J. Fielding3Tobias E. Schrader4Andreas Ostermann5Juliette M. Devos6Pierre Aller7Matthew P. Blakeley8Peter C. E. Moody9Emma L. Raven10Department of Molecular and Cell Biology and Leicester Institute of Structural and Chemical Biology, University of LeicesterDepartment of Molecular and Cell Biology and Leicester Institute of Structural and Chemical Biology, University of LeicesterDepartment of Molecular and Cell Biology and Leicester Institute of Structural and Chemical Biology, University of LeicesterThe Photon Science Institute and School of Chemistry, The University of ManchesterJülich Centre for Neutron Science (JCNS) at Heinz Maier-Leibnitz Zentrum (MLZ), Forschungszentrum Jülich GmbHHeinz Maier-Leibnitz Zentrum (MLZ), Technische Universität MünchenInstitut Laue-LangevinDiamond Light Source Ltd, Diamond House, Harwell Science and Innovation CampusInstitut Laue-LangevinDepartment of Molecular and Cell Biology and Leicester Institute of Structural and Chemical Biology, University of LeicesterDepartment of Chemistry and Leicester Institute of Structural and Chemical Biology, University of LeicesterThe nature of the ferryl intermediate generated in reactions catalysed by heme-containing enzymes is uncertain, due to the ambiguity of X-ray crystallography data. Here, the authors apply neutron diffraction, kinetics and other spectroscopy to directly observe a protonated ferryl intermediate in a heme peroxidase.https://doi.org/10.1038/ncomms13445
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Hanna Kwon Jaswir Basran Cecilia M. Casadei Alistair J. Fielding Tobias E. Schrader Andreas Ostermann Juliette M. Devos Pierre Aller Matthew P. Blakeley Peter C. E. Moody Emma L. Raven
spellingShingle	Hanna Kwon Jaswir Basran Cecilia M. Casadei Alistair J. Fielding Tobias E. Schrader Andreas Ostermann Juliette M. Devos Pierre Aller Matthew P. Blakeley Peter C. E. Moody Emma L. Raven Direct visualization of a Fe(IV)–OH intermediate in a heme enzyme Nature Communications
author_facet	Hanna Kwon Jaswir Basran Cecilia M. Casadei Alistair J. Fielding Tobias E. Schrader Andreas Ostermann Juliette M. Devos Pierre Aller Matthew P. Blakeley Peter C. E. Moody Emma L. Raven
author_sort	Hanna Kwon
title	Direct visualization of a Fe(IV)–OH intermediate in a heme enzyme
title_short	Direct visualization of a Fe(IV)–OH intermediate in a heme enzyme
title_full	Direct visualization of a Fe(IV)–OH intermediate in a heme enzyme
title_fullStr	Direct visualization of a Fe(IV)–OH intermediate in a heme enzyme
title_full_unstemmed	Direct visualization of a Fe(IV)–OH intermediate in a heme enzyme
title_sort	direct visualization of a fe(iv)–oh intermediate in a heme enzyme
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2016-11-01
description	The nature of the ferryl intermediate generated in reactions catalysed by heme-containing enzymes is uncertain, due to the ambiguity of X-ray crystallography data. Here, the authors apply neutron diffraction, kinetics and other spectroscopy to directly observe a protonated ferryl intermediate in a heme peroxidase.
url	https://doi.org/10.1038/ncomms13445
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Direct visualization of a Fe(IV)–OH intermediate in a heme enzyme

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