Study on the mechanism of "mulberry twig-cassia twig" in the treatment of rheumatoid arthritis based on network pharmacology
Objective: To explore the main active components, main target genes and key pathways of "mulberry twig-cassia twig" in the treatment of rheumatoid arthritis based on network pharmacology Methods: The active components and targets of drug pairs were screened by TCMSP database and Swissta...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Editorial Board of Journal of Hainan Medical University
2021-04-01
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| Series: | Journal of Hainan Medical University |
| Subjects: | |
| Online Access: | http://www.hnykdxxb.com/PDF/202108/06.pdf |
| Summary: | Objective: To explore the main active components, main target genes and key pathways
of "mulberry twig-cassia twig" in the treatment of rheumatoid arthritis based on network
pharmacology Methods: The active components and targets of drug pairs were screened by
TCMSP database and Swisstargetprediction database, and the target genes of rheumatoid
arthritis were obtained by GeneCards and OMIM database, and the overlapping genes were
obtained, that is, the predictive target genes of "mulberry twig-cassia twig" medicine for the
treatment of rheumatoid arthritis. The "drug-active ingredient-target" network and "drug-active
ingredient-target-disease" network were constructed by Cytoscape 3.7.2 software. The PPI
network was constructed by String platform and the key biological processes were analyzed
and screened by Cytoscape 3.7.2 software. The GO analysis and KEGG pathway analysis of
predictive target genes were carried out on the Metascape website, and the bubble diagram
was drawn by Omicshare platform. At the same time,those genes can used to construct the
"drug-component-target-pathway" network by Cytoscape 3.7.2 software. Results: A total of
12 active components, 85 predictive targets, 126 KEGG pathways and 1537 GO functions
were obtained. Conclusion: The main active components of "mulberry twig-cassia twig" in
the treatment of rheumatoid arthritis are kaempferol, beta-sitosterol and cinnamic acid, and
the main target genes may be HSP90AB1、RELA, AKT1, TNF, TLR4, PTGS2 and so on.
The main KEGG pathways are TNF signal pathway, estrogen pathway, HIF-1 signal pathway,
NF-κB signal pathway, PI3K-Akt signal pathway and so on. The main GO function analysis
is the response to lipopolysaccharide、response to oxidative stress、cytokine-mediated
signaling pathway.This study shows that the "mulberry twig-cassia twig" drug pair is through
the regulation of multip-compound, multi-target, multi-pathway to treat rheumatoid arthritis,
providing a theoretical basis for the follow-up study of traditional Chinese medicine in the
treatment of rheumatoid arthritis. |
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| ISSN: | 1007-1237 1007-1237 |