Estrogen Replacement Reduces Hearing Threshold Shifts and Cochlear Hair Cell Loss After Acoustic Overexposure in Ovariectomized Rats
Objectives. The relationship of estrogen (the primary female sex hormone) with hearing function has been studied in both humans and animals. However, whether estrogen levels affect hearing remains uncertain. Therefore, in this study, we investigated changes in the vulnerability of hearing to acousti...
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Korean Society of Otorhinolaryngology-Head and Neck Surgery
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doaj-ba1dd718a3a34d47b9ca553b832f602d2021-05-10T04:30:01ZengKorean Society of Otorhinolaryngology-Head and Neck SurgeryClinical and Experimental Otorhinolaryngology1976-87102005-07202021-02-01141616810.21053/ceo.2019.01662705Estrogen Replacement Reduces Hearing Threshold Shifts and Cochlear Hair Cell Loss After Acoustic Overexposure in Ovariectomized RatsMin Tae Kim0Jae-Hun Lee1Nathaniel T. Carpena2Min Young Lee3Phil-Sang Chung4Jae Yun Jung5 Department of Otolaryngology-Head and Neck Surgery, Dankook University Hospital, Cheonan, Korea Department of Otolaryngology-Head and Neck Surgery, Dankook University Hospital, Cheonan, Korea Department of Otolaryngology-Head and Neck Surgery, Dankook University College of Medicine, Cheonan, Korea Department of Otolaryngology-Head and Neck Surgery, Dankook University Hospital, Cheonan, Korea Department of Otolaryngology-Head and Neck Surgery, Dankook University Hospital, Cheonan, Korea Department of Otolaryngology-Head and Neck Surgery, Dankook University Hospital, Cheonan, KoreaObjectives. The relationship of estrogen (the primary female sex hormone) with hearing function has been studied in both humans and animals. However, whether estrogen levels affect hearing remains uncertain. Therefore, in this study, we investigated changes in the vulnerability of hearing to acoustic overexposure in ovariectomized female rats. Methods. Eighteen 8-week-old female Sprague-Dawley rats were separated into four groups as follows: sham ovariectomy (OP), OP only, and OP treated with low (10 µg/kg) or high doses (100 µg/kg) of estrogen. Rats in the estrogen replacement groups were given two intraperitoneal injections. Hearing thresholds were measured before noise exposure, and at 1 day and 2 weeks after exposure. Results. The hearing thresholds of the sham OP and OP-only groups were not significantly different. However, both estrogen groups showed a lower threshold shift than the OP-only group. Histological immunostaining analyses showed that hair cell loss in the 32 kHz region was more severe in the sham OP group than in the OP-only group. Furthermore, there was little or no hair cell loss in either estrogen replacement group and significantly more hair cell loss in the OP-only group. Conclusion. These results suggest that estrogen replacement may reduce the vulnerability of hearing to noise exposure in menopausal women.http://www.e-ceo.org/upload/pdf/ceo-2019-01662.pdfhearing lossestrogen replacement therapyovariectomy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Min Tae Kim Jae-Hun Lee Nathaniel T. Carpena Min Young Lee Phil-Sang Chung Jae Yun Jung |
spellingShingle |
Min Tae Kim Jae-Hun Lee Nathaniel T. Carpena Min Young Lee Phil-Sang Chung Jae Yun Jung Estrogen Replacement Reduces Hearing Threshold Shifts and Cochlear Hair Cell Loss After Acoustic Overexposure in Ovariectomized Rats Clinical and Experimental Otorhinolaryngology hearing loss estrogen replacement therapy ovariectomy |
author_facet |
Min Tae Kim Jae-Hun Lee Nathaniel T. Carpena Min Young Lee Phil-Sang Chung Jae Yun Jung |
author_sort |
Min Tae Kim |
title |
Estrogen Replacement Reduces Hearing Threshold Shifts and Cochlear Hair Cell Loss After Acoustic Overexposure in Ovariectomized Rats |
title_short |
Estrogen Replacement Reduces Hearing Threshold Shifts and Cochlear Hair Cell Loss After Acoustic Overexposure in Ovariectomized Rats |
title_full |
Estrogen Replacement Reduces Hearing Threshold Shifts and Cochlear Hair Cell Loss After Acoustic Overexposure in Ovariectomized Rats |
title_fullStr |
Estrogen Replacement Reduces Hearing Threshold Shifts and Cochlear Hair Cell Loss After Acoustic Overexposure in Ovariectomized Rats |
title_full_unstemmed |
Estrogen Replacement Reduces Hearing Threshold Shifts and Cochlear Hair Cell Loss After Acoustic Overexposure in Ovariectomized Rats |
title_sort |
estrogen replacement reduces hearing threshold shifts and cochlear hair cell loss after acoustic overexposure in ovariectomized rats |
publisher |
Korean Society of Otorhinolaryngology-Head and Neck Surgery |
series |
Clinical and Experimental Otorhinolaryngology |
issn |
1976-8710 2005-0720 |
publishDate |
2021-02-01 |
description |
Objectives. The relationship of estrogen (the primary female sex hormone) with hearing function has been studied in both humans and animals. However, whether estrogen levels affect hearing remains uncertain. Therefore, in this study, we investigated changes in the vulnerability of hearing to acoustic overexposure in ovariectomized female rats. Methods. Eighteen 8-week-old female Sprague-Dawley rats were separated into four groups as follows: sham ovariectomy (OP), OP only, and OP treated with low (10 µg/kg) or high doses (100 µg/kg) of estrogen. Rats in the estrogen replacement groups were given two intraperitoneal injections. Hearing thresholds were measured before noise exposure, and at 1 day and 2 weeks after exposure. Results. The hearing thresholds of the sham OP and OP-only groups were not significantly different. However, both estrogen groups showed a lower threshold shift than the OP-only group. Histological immunostaining analyses showed that hair cell loss in the 32 kHz region was more severe in the sham OP group than in the OP-only group. Furthermore, there was little or no hair cell loss in either estrogen replacement group and significantly more hair cell loss in the OP-only group. Conclusion. These results suggest that estrogen replacement may reduce the vulnerability of hearing to noise exposure in menopausal women. |
topic |
hearing loss estrogen replacement therapy ovariectomy |
url |
http://www.e-ceo.org/upload/pdf/ceo-2019-01662.pdf |
work_keys_str_mv |
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