Relationship of GPRC6A and PKCzeta expression levels in prostate cancer lesions with cell proliferation and EMT

• Main Authors: Fan-Fei Zeng, Sheng-Ping Tian, Wei-Zhong Yang, Xiao-Yue Zhang
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2017-08-01
• Series: Journal of Hainan Medical University
• Subjects: Prostate cancer; G-protein-coupled receptor C6A; Protein kinase Cζ; Proliferation; Epithelial-mesenchymal transition
• Online Access: http://www.hnykdxxb.com/PDF/201715/35.pdf

Objective: To study the relationship of GPRC6A and PKCzeta expression levels in prostate cancer lesions with cell proliferation and epithelial-mesenchymal transition (EMT). Methods: Patients with prostate cancer and patients with benign prostatic hyperplasia who received surgical resection in Huizhou Third People’s Hospital between June 2014 and March 2017 were selected, right amount of prostate cancer tissue and tissue adjacent to carcinoma was collected from patients with prostate cancer, and the expression of GPRC6A, PKCzeta, cell proliferation genes and EMT genes in lesions were detected. Results: GPRC6A, Survivin, SRSF1, Bcl-xl, N-cadherin and Vimentin expression in prostate cancer lesions and adjacent lesions were significantly higher than those in benign prostatic hyperplasia lesions while PKCzeta, Caspase-3, Caspase-9, Apaf-1, E-cadherin and CK5/6 expression were significantly lower than those in benign prostatic hyperplasia lesions; Survivin, SRSF1 and Bcl-xl expression in prostate cancer lesions with lower PKCzeta expression were significantly higher than those in prostate cancer lesions with higher PKCzeta expression while Caspase-3, Caspase-9 and Apaf-1 expression were significantly lower than those in prostate cancer lesions with higher PKCzeta expression; E-cadherin and CK5/6 expression in prostate cancer lesions with lower GPRC6A expression were significantly higher than those in prostate cancer lesions with higher GPRC6A expression while N-cadherin and Vimentin expression were significantly lower than those in prostate cancer lesions with higher GPRC6A expression. Conclusion: Highly expressed GPRC6A and lowly expressed PKCzeta in prostate cancer lesions can promote cell EMT and proliferation respectively