Nup98 FG domains from diverse species spontaneously phase-separate into particles with nuclear pore-like permselectivity
Nuclear pore complexes (NPCs) conduct massive transport mediated by shuttling nuclear transport receptors (NTRs), while keeping nuclear and cytoplasmic contents separated. The NPC barrier in Xenopus relies primarily on the intrinsically disordered FG domain of Nup98. We now observed that Nup98 FG do...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
eLife Sciences Publications Ltd
2015-01-01
|
Series: | eLife |
Subjects: | |
Online Access: | https://elifesciences.org/articles/04251 |
id |
doaj-ba303fc7a73a4bc7af69b98165d2b352 |
---|---|
record_format |
Article |
spelling |
doaj-ba303fc7a73a4bc7af69b98165d2b3522021-05-04T23:36:53ZengeLife Sciences Publications LtdeLife2050-084X2015-01-01410.7554/eLife.04251Nup98 FG domains from diverse species spontaneously phase-separate into particles with nuclear pore-like permselectivityHermann Broder Schmidt0Dirk Görlich1Department of Cellular Logistics, Max Planck Institute for Biophysical Chemistry, Göttingen, GermanyDepartment of Cellular Logistics, Max Planck Institute for Biophysical Chemistry, Göttingen, GermanyNuclear pore complexes (NPCs) conduct massive transport mediated by shuttling nuclear transport receptors (NTRs), while keeping nuclear and cytoplasmic contents separated. The NPC barrier in Xenopus relies primarily on the intrinsically disordered FG domain of Nup98. We now observed that Nup98 FG domains of mammals, lancelets, insects, nematodes, fungi, plants, amoebas, ciliates, and excavates spontaneously and rapidly phase-separate from dilute (submicromolar) aqueous solutions into characteristic ‘FG particles’. This required neither sophisticated experimental conditions nor auxiliary eukaryotic factors. Instead, it occurred already during FG domain expression in bacteria. All Nup98 FG phases rejected inert macromolecules and yet allowed far larger NTR cargo complexes to rapidly enter. They even recapitulated the observations that large cargo-domains counteract NPC passage of NTR⋅cargo complexes, while cargo shielding and increased NTR⋅cargo surface-ratios override this inhibition. Their exquisite NPC-typical sorting selectivity and strong intrinsic assembly propensity suggest that Nup98 FG phases can form in authentic NPCs and indeed account for the permeability properties of the pore.https://elifesciences.org/articles/04251Tetrahymenaimportinnucleoporinnucleocytoplasmic transportNup100Nup116 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hermann Broder Schmidt Dirk Görlich |
spellingShingle |
Hermann Broder Schmidt Dirk Görlich Nup98 FG domains from diverse species spontaneously phase-separate into particles with nuclear pore-like permselectivity eLife Tetrahymena importin nucleoporin nucleocytoplasmic transport Nup100 Nup116 |
author_facet |
Hermann Broder Schmidt Dirk Görlich |
author_sort |
Hermann Broder Schmidt |
title |
Nup98 FG domains from diverse species spontaneously phase-separate into particles with nuclear pore-like permselectivity |
title_short |
Nup98 FG domains from diverse species spontaneously phase-separate into particles with nuclear pore-like permselectivity |
title_full |
Nup98 FG domains from diverse species spontaneously phase-separate into particles with nuclear pore-like permselectivity |
title_fullStr |
Nup98 FG domains from diverse species spontaneously phase-separate into particles with nuclear pore-like permselectivity |
title_full_unstemmed |
Nup98 FG domains from diverse species spontaneously phase-separate into particles with nuclear pore-like permselectivity |
title_sort |
nup98 fg domains from diverse species spontaneously phase-separate into particles with nuclear pore-like permselectivity |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2015-01-01 |
description |
Nuclear pore complexes (NPCs) conduct massive transport mediated by shuttling nuclear transport receptors (NTRs), while keeping nuclear and cytoplasmic contents separated. The NPC barrier in Xenopus relies primarily on the intrinsically disordered FG domain of Nup98. We now observed that Nup98 FG domains of mammals, lancelets, insects, nematodes, fungi, plants, amoebas, ciliates, and excavates spontaneously and rapidly phase-separate from dilute (submicromolar) aqueous solutions into characteristic ‘FG particles’. This required neither sophisticated experimental conditions nor auxiliary eukaryotic factors. Instead, it occurred already during FG domain expression in bacteria. All Nup98 FG phases rejected inert macromolecules and yet allowed far larger NTR cargo complexes to rapidly enter. They even recapitulated the observations that large cargo-domains counteract NPC passage of NTR⋅cargo complexes, while cargo shielding and increased NTR⋅cargo surface-ratios override this inhibition. Their exquisite NPC-typical sorting selectivity and strong intrinsic assembly propensity suggest that Nup98 FG phases can form in authentic NPCs and indeed account for the permeability properties of the pore. |
topic |
Tetrahymena importin nucleoporin nucleocytoplasmic transport Nup100 Nup116 |
url |
https://elifesciences.org/articles/04251 |
work_keys_str_mv |
AT hermannbroderschmidt nup98fgdomainsfromdiversespeciesspontaneouslyphaseseparateintoparticleswithnuclearporelikepermselectivity AT dirkgorlich nup98fgdomainsfromdiversespeciesspontaneouslyphaseseparateintoparticleswithnuclearporelikepermselectivity |
_version_ |
1721476893422649344 |