Estradiol- and testosterone-induced alterations in phosphatidylcholine and triglyceride synthesis in hepatic endoplasmic reticulum
Pathways of phosphatidylcholine and triglyceride biosynthesis were studied in hepatic endoplasmic reticulum from castrated and noncastrated male rats pretreated with estradiol or testosterone.In vitro measurements of hepatic microsomal enzymes which catalyze phosphatidylcholine biosynthesis revealed...
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1971-09-01
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520394785 |
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doaj-ba43694db671438398a6dc8bfc4e10782021-04-24T05:51:56ZengElsevierJournal of Lipid Research0022-22751971-09-01125590595Estradiol- and testosterone-induced alterations in phosphatidylcholine and triglyceride synthesis in hepatic endoplasmic reticulumDAVID L. YOUNG0Department of Medicine, Duke University Medical Center, Durham, North Carolina 27706Pathways of phosphatidylcholine and triglyceride biosynthesis were studied in hepatic endoplasmic reticulum from castrated and noncastrated male rats pretreated with estradiol or testosterone.In vitro measurements of hepatic microsomal enzymes which catalyze phosphatidylcholine biosynthesis revealed a significant increase in the specific activity of the enzyme governing phosphatidylcholine biosynthesis by the sequential methylation of phosphatidylethanolamine in the estradiol-treated castrate animals. The specific activity of phosphorylcholine–glyceride transferase was decreased by estradiol treatment in both castrate and noncastrate animals. The specific activity of diglyceride acyltransferase, which catalyzes triglyceride biosynthesis, was decreased by estradiol pretreatment in both castrate and noncastrate animals and was increased by testosterone in the castrate animals.The changes in specific activity of the enzymes governing phosphatidylcholine biosynthesis may account for the previously noted increased in vivo incorporation of methyl groups of l-methionine into hepatic phosphatidylcholine in female and estradiol-treated animals; the data suggest that in female and estradiol-treated rats a greater proportion of hepatic phosphatidylcholine is synthesized by the stepwise methylation of phosphatidylethanolamine. The decrease in diglyceride acyltransferase specific activity seen after estradiol administration may account for the lipotropic-like effect of estradiol.http://www.sciencedirect.com/science/article/pii/S0022227520394785S-adenosyl-l-methionine:phosphatidylethanolamine methyltransferasephosphorylcholine–glyceride transferasediglyceride acyltransferasecholinel-methionineCDP-choline |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
DAVID L. YOUNG |
spellingShingle |
DAVID L. YOUNG Estradiol- and testosterone-induced alterations in phosphatidylcholine and triglyceride synthesis in hepatic endoplasmic reticulum Journal of Lipid Research S-adenosyl-l-methionine:phosphatidylethanolamine methyltransferase phosphorylcholine–glyceride transferase diglyceride acyltransferase choline l-methionine CDP-choline |
author_facet |
DAVID L. YOUNG |
author_sort |
DAVID L. YOUNG |
title |
Estradiol- and testosterone-induced alterations in phosphatidylcholine and triglyceride synthesis in hepatic endoplasmic reticulum |
title_short |
Estradiol- and testosterone-induced alterations in phosphatidylcholine and triglyceride synthesis in hepatic endoplasmic reticulum |
title_full |
Estradiol- and testosterone-induced alterations in phosphatidylcholine and triglyceride synthesis in hepatic endoplasmic reticulum |
title_fullStr |
Estradiol- and testosterone-induced alterations in phosphatidylcholine and triglyceride synthesis in hepatic endoplasmic reticulum |
title_full_unstemmed |
Estradiol- and testosterone-induced alterations in phosphatidylcholine and triglyceride synthesis in hepatic endoplasmic reticulum |
title_sort |
estradiol- and testosterone-induced alterations in phosphatidylcholine and triglyceride synthesis in hepatic endoplasmic reticulum |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
1971-09-01 |
description |
Pathways of phosphatidylcholine and triglyceride biosynthesis were studied in hepatic endoplasmic reticulum from castrated and noncastrated male rats pretreated with estradiol or testosterone.In vitro measurements of hepatic microsomal enzymes which catalyze phosphatidylcholine biosynthesis revealed a significant increase in the specific activity of the enzyme governing phosphatidylcholine biosynthesis by the sequential methylation of phosphatidylethanolamine in the estradiol-treated castrate animals. The specific activity of phosphorylcholine–glyceride transferase was decreased by estradiol treatment in both castrate and noncastrate animals. The specific activity of diglyceride acyltransferase, which catalyzes triglyceride biosynthesis, was decreased by estradiol pretreatment in both castrate and noncastrate animals and was increased by testosterone in the castrate animals.The changes in specific activity of the enzymes governing phosphatidylcholine biosynthesis may account for the previously noted increased in vivo incorporation of methyl groups of l-methionine into hepatic phosphatidylcholine in female and estradiol-treated animals; the data suggest that in female and estradiol-treated rats a greater proportion of hepatic phosphatidylcholine is synthesized by the stepwise methylation of phosphatidylethanolamine. The decrease in diglyceride acyltransferase specific activity seen after estradiol administration may account for the lipotropic-like effect of estradiol. |
topic |
S-adenosyl-l-methionine:phosphatidylethanolamine methyltransferase phosphorylcholine–glyceride transferase diglyceride acyltransferase choline l-methionine CDP-choline |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520394785 |
work_keys_str_mv |
AT davidlyoung estradiolandtestosteroneinducedalterationsinphosphatidylcholineandtriglyceridesynthesisinhepaticendoplasmicreticulum |
_version_ |
1721511614221385728 |