Characterisation of Casein Kinase 1.1 in Leishmania donovani Using the CRISPR Cas9 Toolkit
The recent adaptation of CRISPR Cas9 genome editing to Leishmania spp. has opened a new era in deciphering Leishmania biology. The method was recently improved using a PCR-based CRISPR Cas9 approach, which eliminated the need for cloning. This new approach, which allows high-throughput gene deletion...
Main Authors: | Daniel Martel, Tom Beneke, Eva Gluenz, Gerald F. Späth, Najma Rachidi |
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Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2017-01-01
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Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2017/4635605 |
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