Effects of culture conditions on the production of inulinase by Kluyveromyces marxianus
The present study was conducted to investigate the influence of initial sucrose concentration, pH and aeration rate on biomass and inulinase production by Kluyveromyces marxianus var. bulgaricus in a stirred batch reactor. Maximum inulinase activity (15.29 UmL-1) was obtained at a sucrose concentrat...
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Instituto de Tecnologia do Paraná (Tecpar)
2010-06-01
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doaj-ba6ce3ea40b347e49656afd06b1c35902020-11-25T02:09:16ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242010-06-0153370170710.1590/S1516-89132010000300027Effects of culture conditions on the production of inulinase by Kluyveromyces marxianusMarcia Luciana CazettaRubens MontiJonas ContieroThe present study was conducted to investigate the influence of initial sucrose concentration, pH and aeration rate on biomass and inulinase production by Kluyveromyces marxianus var. bulgaricus in a stirred batch reactor. Maximum inulinase activity (15.29 UmL-1) was obtained at a sucrose concentration of 10 g L-1, pH 5.0 and aeration rate of 1 vvm. The 20 g L-1 sucrose concentration was suitable for cell growth; however, enzymatic activity at this concentration was inhibited due to catabolic repression. The increase in aeration rate caused a reduction in enzyme activity with no relevant biomass increase.<br>O estudo foi conduzido para investigar a influência da concentração inicial da sacarose, a taxa da aeração e do pH na biomassa e na produção da inulinase pela Kluyveromyces marxianus var. bulgaricus em um reator em batelada. A máxima atividade de inulinase, 15.29 UmL-1, foi obtida na concentração de 10 g L-1 de sacarose, no pH 5.0 e na taxa da aeração de 1 vvm. A concentração de sacarose de 20g L-1 foi apropriada para o crescimento celular, porém nesta concentração a atividade enzimática foi inibida, devido a repressão catabólica. O aumento na taxa da aeração propiciou redução da atividade enzimática, ao mesmo tempo em que não houve aumento considerável do biomassa.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300027Kluyveromyces marxianus var. bulgaricussucroseinulinasebiomassbatch fermentation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marcia Luciana Cazetta Rubens Monti Jonas Contiero |
spellingShingle |
Marcia Luciana Cazetta Rubens Monti Jonas Contiero Effects of culture conditions on the production of inulinase by Kluyveromyces marxianus Brazilian Archives of Biology and Technology Kluyveromyces marxianus var. bulgaricus sucrose inulinase biomass batch fermentation |
author_facet |
Marcia Luciana Cazetta Rubens Monti Jonas Contiero |
author_sort |
Marcia Luciana Cazetta |
title |
Effects of culture conditions on the production of inulinase by Kluyveromyces marxianus |
title_short |
Effects of culture conditions on the production of inulinase by Kluyveromyces marxianus |
title_full |
Effects of culture conditions on the production of inulinase by Kluyveromyces marxianus |
title_fullStr |
Effects of culture conditions on the production of inulinase by Kluyveromyces marxianus |
title_full_unstemmed |
Effects of culture conditions on the production of inulinase by Kluyveromyces marxianus |
title_sort |
effects of culture conditions on the production of inulinase by kluyveromyces marxianus |
publisher |
Instituto de Tecnologia do Paraná (Tecpar) |
series |
Brazilian Archives of Biology and Technology |
issn |
1516-8913 1678-4324 |
publishDate |
2010-06-01 |
description |
The present study was conducted to investigate the influence of initial sucrose concentration, pH and aeration rate on biomass and inulinase production by Kluyveromyces marxianus var. bulgaricus in a stirred batch reactor. Maximum inulinase activity (15.29 UmL-1) was obtained at a sucrose concentration of 10 g L-1, pH 5.0 and aeration rate of 1 vvm. The 20 g L-1 sucrose concentration was suitable for cell growth; however, enzymatic activity at this concentration was inhibited due to catabolic repression. The increase in aeration rate caused a reduction in enzyme activity with no relevant biomass increase.<br>O estudo foi conduzido para investigar a influência da concentração inicial da sacarose, a taxa da aeração e do pH na biomassa e na produção da inulinase pela Kluyveromyces marxianus var. bulgaricus em um reator em batelada. A máxima atividade de inulinase, 15.29 UmL-1, foi obtida na concentração de 10 g L-1 de sacarose, no pH 5.0 e na taxa da aeração de 1 vvm. A concentração de sacarose de 20g L-1 foi apropriada para o crescimento celular, porém nesta concentração a atividade enzimática foi inibida, devido a repressão catabólica. O aumento na taxa da aeração propiciou redução da atividade enzimática, ao mesmo tempo em que não houve aumento considerável do biomassa. |
topic |
Kluyveromyces marxianus var. bulgaricus sucrose inulinase biomass batch fermentation |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300027 |
work_keys_str_mv |
AT marcialucianacazetta effectsofcultureconditionsontheproductionofinulinasebykluyveromycesmarxianus AT rubensmonti effectsofcultureconditionsontheproductionofinulinasebykluyveromycesmarxianus AT jonascontiero effectsofcultureconditionsontheproductionofinulinasebykluyveromycesmarxianus |
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