Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept
Isolation of the pathogens Yersinia enterocolitica and Yersinia pseudotuberculosis from foods typically rely on slow (10–21 day) “cold enrichment” protocols before confirmed results are obtained. We describe an approach that yields results in 39 h that combines an alternative enrichment method with...
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2021-02-01
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doaj-ba72b08dc7e346dea1e4eb64694d95662021-02-17T06:36:09ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-02-011210.3389/fmicb.2021.641801641801Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of ConceptStephen L. W. On0Yuwei Zhang1Andrew Gehring2Valery Patsekin3Venkata Chelikani4Steve Flint5Haoran Wang6Craig Billington7Graham C. Fletcher8James Lindsay9J. Paul Robinson10Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, New ZealandDepartment of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, New ZealandEastern Regional Research Center, Agricultural Research Service, USDA, Wyndmoor, PA, United StatesDepartment of Basic Medical Sciences, Purdue University, West Lafayette, IN, United StatesDepartment of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, New ZealandSchool of Food and Advanced Technology, Massey University, Palmerston North, New ZealandSchool of Food and Advanced Technology, Massey University, Palmerston North, New ZealandInstitute of Environmental Science and Research, Christchurch, New ZealandNew Zealand Institute for Plant & Food Research Limited, Auckland, New ZealandAgricultural Research Service, Office of National Programs, USDA, Washington, DC, United StatesWeldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, United StatesIsolation of the pathogens Yersinia enterocolitica and Yersinia pseudotuberculosis from foods typically rely on slow (10–21 day) “cold enrichment” protocols before confirmed results are obtained. We describe an approach that yields results in 39 h that combines an alternative enrichment method with culture on a non-selective medium, and subsequent identification of suspect colonies using elastic light scatter (ELS) analysis. A prototype database of ELS profiles from five Yersinia species and six other bacterial genera found in pork mince was established, and used to compare similar profiles of colonies obtained from enrichment cultures from pork mince samples seeded with representative strains of Y. enterocolitica and Y. pseudotuberculosis. The presumptive identification by ELS using computerised or visual analyses of 83/90 colonies in these experiments as the target species was confirmed by partial 16S rDNA sequencing. In addition to seeded cultures, our method recovered two naturally occurring Yersinia strains. Our results indicate that modified enrichment combined with ELS is a promising new approach for expedited detection of foodborne pathogenic yersiniae.https://www.frontiersin.org/articles/10.3389/fmicb.2021.641801/fullYersiniadiagnosticsdetectionelastic light scatterfood |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Stephen L. W. On Yuwei Zhang Andrew Gehring Valery Patsekin Venkata Chelikani Steve Flint Haoran Wang Craig Billington Graham C. Fletcher James Lindsay J. Paul Robinson |
spellingShingle |
Stephen L. W. On Yuwei Zhang Andrew Gehring Valery Patsekin Venkata Chelikani Steve Flint Haoran Wang Craig Billington Graham C. Fletcher James Lindsay J. Paul Robinson Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept Frontiers in Microbiology Yersinia diagnostics detection elastic light scatter food |
author_facet |
Stephen L. W. On Yuwei Zhang Andrew Gehring Valery Patsekin Venkata Chelikani Steve Flint Haoran Wang Craig Billington Graham C. Fletcher James Lindsay J. Paul Robinson |
author_sort |
Stephen L. W. On |
title |
Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept |
title_short |
Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept |
title_full |
Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept |
title_fullStr |
Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept |
title_full_unstemmed |
Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept |
title_sort |
elastic light scatter pattern analysis for the expedited detection of yersinia species in pork mince: proof of concept |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2021-02-01 |
description |
Isolation of the pathogens Yersinia enterocolitica and Yersinia pseudotuberculosis from foods typically rely on slow (10–21 day) “cold enrichment” protocols before confirmed results are obtained. We describe an approach that yields results in 39 h that combines an alternative enrichment method with culture on a non-selective medium, and subsequent identification of suspect colonies using elastic light scatter (ELS) analysis. A prototype database of ELS profiles from five Yersinia species and six other bacterial genera found in pork mince was established, and used to compare similar profiles of colonies obtained from enrichment cultures from pork mince samples seeded with representative strains of Y. enterocolitica and Y. pseudotuberculosis. The presumptive identification by ELS using computerised or visual analyses of 83/90 colonies in these experiments as the target species was confirmed by partial 16S rDNA sequencing. In addition to seeded cultures, our method recovered two naturally occurring Yersinia strains. Our results indicate that modified enrichment combined with ELS is a promising new approach for expedited detection of foodborne pathogenic yersiniae. |
topic |
Yersinia diagnostics detection elastic light scatter food |
url |
https://www.frontiersin.org/articles/10.3389/fmicb.2021.641801/full |
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