Immunogenicity of HLA-DR1 Restricted Peptides Derived from Leishmania Major gp63 Using FVB/N-DR1 Transgenic Mouse Model

Immunogenicity of HLA-DR1 Restricted Peptides Derived from Leishmania Major gp63 Using FVB/N-DR1 Transgenic Mouse Model

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Background: Leishmaniasis is a worldwide disease prevalent in tropical and sub- tropical countries. In the present study the immunogenicity of three human HLA-DR1 restricted peptides derived from L. major gp63 protein was evaluated using FVB/N-DR1 transgenic mouse model.Methods: The immunity generat...

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Bibliographic Details
Main Author: H Rezvan
Format: Article
Language:English
Published: Tehran University of Medical Sciences 2013-06-01
Series:Iranian Journal of Parasitology
Subjects:
Leishmania Major
gp63
IFN-γ
Peptide
Proliferation
FVB/N-DR1 Transgenic Mice
Online Access:http://ijpa.tums.ac.ir/index.php/ijpa/article/view/630/405
Description
Summary:Background: Leishmaniasis is a worldwide disease prevalent in tropical and sub- tropical countries. In the present study the immunogenicity of three human HLA-DR1 restricted peptides derived from L. major gp63 protein was evaluated using FVB/N-DR1 transgenic mouse model.Methods: The immunity generated by three MHC class II – restricted peptides with the sequence of AARLVRLAAAGAAVT (AAR), AAPLVRLAAAGAAVT (AAP) and SRYDQLVTRVVTHE (ASR) derived from L. major gp63 protein were predicted using a web-based software (SYFPEITHI) and tested in FVB/N-DR1 transgenic mice.Results: Immunization of FVB/N-DR1 transgenic mice with one of the three predicted peptides (AAR) resulted in high levels of Th1-type immune response as well as significant levels of IFN-γ de­tected by Proliferation assay and ELISA.Conclusion: The results indicate a high level of immunogenicity for AAR, which can be a potent candidate for peptide vaccine in Leishmania infections.
ISSN:1735-7020
2008-238X

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