Sodium-dependent phosphate transporter NaPi2b as a potential predictive marker for targeted therapy of ovarian cancer
The high mortality rate from ovarian cancer is due to the asymptomatic nature of the course of the disease, which leads to the diagnosis of ovarian cancer in later stages. The sodium-dependent phosphate transporter NaPi2b encoded by SLC34A2 gene is expressed in 80–90% of epithelial ovarian cancers a...
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doaj-ba740cee4e8e4e12bb6a6759d94903722021-09-03T04:46:28ZengElsevierBiochemistry and Biophysics Reports2405-58082021-12-0128101104Sodium-dependent phosphate transporter NaPi2b as a potential predictive marker for targeted therapy of ovarian cancerA.K. Nurgalieva0V.E. Popov1V.S. Skripova2L.F. Bulatova3D.V. Savenkova4R.A. Vlasenkova5S.Z. Safina6E. Zh Shakirova7V.V. Filonenko8M.V. Bogdanov9R.G. Kiyamova10Research Laboratory “Biomarker”, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, 420008, Russian FederationResearch Laboratory “Biomarker”, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, 420008, Russian FederationResearch Laboratory “Biomarker”, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, 420008, Russian FederationResearch Laboratory “Biomarker”, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, 420008, Russian FederationResearch Laboratory “Biomarker”, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, 420008, Russian FederationResearch Laboratory “Biomarker”, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, 420008, Russian FederationRepublican Clinical Oncological Dispensary, Kazan, 420029, Russian FederationRepublican Clinical Oncological Dispensary, Kazan, 420029, Russian Federation; Department of Surgery, Obstetrics, and Gynecology, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, 420008, Russian FederationInstitute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv, 03680, UkraineDepartment of Biochemistry and Molecular Biology, University of Texas-Houston, McGovern Medical School, Houston, TX, 77225, USAResearch Laboratory “Biomarker”, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, 420008, Russian Federation; Corresponding author.The high mortality rate from ovarian cancer is due to the asymptomatic nature of the course of the disease, which leads to the diagnosis of ovarian cancer in later stages. The sodium-dependent phosphate transporter NaPi2b encoded by SLC34A2 gene is expressed in 80–90% of epithelial ovarian cancers and used as a target for therapeutic antibodies XMT-1536, and XMT-1592, which are derived from MX35 antibodies and used in clinical trials for the treatment of ovarian and lung cancers. In this work, we aimed to evaluate NaPi2b as a molecular marker for diagnostics and predicting the course and outcome of ovarian cancer disease.Quantitative analysis of SLC34A2 gene expression in ovarian tumor tissue was performed at the level of transcription and translation using real-time PCR, droplet digital PCR and Western blot analysis respectively. Statistical analysis was performed taking into account various clinicopathological characteristics of the ovarian cancer patients, including the stage of the disease, the tumor grade, the applying of neoadjuvant chemotherapy and the presence of ascites. In this work, we demonstrated that the expression of the human NaPi2b (hNaPi2b) transporter is downregulated in the tumors of patients receiving neoadjuvant therapy and during the development of disease. The data suggest that the level of expression of the SLC34A2 gene can serve as a potential marker for the monitoring and predicting responses to neoadjuvant and targeted therapy in patients with ovarian cancer.http://www.sciencedirect.com/science/article/pii/S2405580821001990Ovarian cancerMolecular markerPredictive markerNeoadjuvant therapyNaPi2bSLC34A2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
A.K. Nurgalieva V.E. Popov V.S. Skripova L.F. Bulatova D.V. Savenkova R.A. Vlasenkova S.Z. Safina E. Zh Shakirova V.V. Filonenko M.V. Bogdanov R.G. Kiyamova |
spellingShingle |
A.K. Nurgalieva V.E. Popov V.S. Skripova L.F. Bulatova D.V. Savenkova R.A. Vlasenkova S.Z. Safina E. Zh Shakirova V.V. Filonenko M.V. Bogdanov R.G. Kiyamova Sodium-dependent phosphate transporter NaPi2b as a potential predictive marker for targeted therapy of ovarian cancer Biochemistry and Biophysics Reports Ovarian cancer Molecular marker Predictive marker Neoadjuvant therapy NaPi2b SLC34A2 |
author_facet |
A.K. Nurgalieva V.E. Popov V.S. Skripova L.F. Bulatova D.V. Savenkova R.A. Vlasenkova S.Z. Safina E. Zh Shakirova V.V. Filonenko M.V. Bogdanov R.G. Kiyamova |
author_sort |
A.K. Nurgalieva |
title |
Sodium-dependent phosphate transporter NaPi2b as a potential predictive marker for targeted therapy of ovarian cancer |
title_short |
Sodium-dependent phosphate transporter NaPi2b as a potential predictive marker for targeted therapy of ovarian cancer |
title_full |
Sodium-dependent phosphate transporter NaPi2b as a potential predictive marker for targeted therapy of ovarian cancer |
title_fullStr |
Sodium-dependent phosphate transporter NaPi2b as a potential predictive marker for targeted therapy of ovarian cancer |
title_full_unstemmed |
Sodium-dependent phosphate transporter NaPi2b as a potential predictive marker for targeted therapy of ovarian cancer |
title_sort |
sodium-dependent phosphate transporter napi2b as a potential predictive marker for targeted therapy of ovarian cancer |
publisher |
Elsevier |
series |
Biochemistry and Biophysics Reports |
issn |
2405-5808 |
publishDate |
2021-12-01 |
description |
The high mortality rate from ovarian cancer is due to the asymptomatic nature of the course of the disease, which leads to the diagnosis of ovarian cancer in later stages. The sodium-dependent phosphate transporter NaPi2b encoded by SLC34A2 gene is expressed in 80–90% of epithelial ovarian cancers and used as a target for therapeutic antibodies XMT-1536, and XMT-1592, which are derived from MX35 antibodies and used in clinical trials for the treatment of ovarian and lung cancers. In this work, we aimed to evaluate NaPi2b as a molecular marker for diagnostics and predicting the course and outcome of ovarian cancer disease.Quantitative analysis of SLC34A2 gene expression in ovarian tumor tissue was performed at the level of transcription and translation using real-time PCR, droplet digital PCR and Western blot analysis respectively. Statistical analysis was performed taking into account various clinicopathological characteristics of the ovarian cancer patients, including the stage of the disease, the tumor grade, the applying of neoadjuvant chemotherapy and the presence of ascites. In this work, we demonstrated that the expression of the human NaPi2b (hNaPi2b) transporter is downregulated in the tumors of patients receiving neoadjuvant therapy and during the development of disease. The data suggest that the level of expression of the SLC34A2 gene can serve as a potential marker for the monitoring and predicting responses to neoadjuvant and targeted therapy in patients with ovarian cancer. |
topic |
Ovarian cancer Molecular marker Predictive marker Neoadjuvant therapy NaPi2b SLC34A2 |
url |
http://www.sciencedirect.com/science/article/pii/S2405580821001990 |
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