A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas
Cluster of differentiation 63 (CD63), a four-transmembrane glycoprotein in the subfamily of tetraspanin, has been widely recognized as a gateway from the infection of foreign invaders to the immune defense of hosts. Its role in Pacific oyster Crassostrea gigas is, however, yet to be discovered. This...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-07-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2020.01379/full |
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doaj-ba846b904c004f5bbc3d422aa7cec733 |
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| record_format |
Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Conghui Liu Conghui Liu Conghui Liu Chuanyan Yang Chuanyan Yang Chuanyan Yang Mengqiang Wang Shuai Jiang Qilin Yi Qilin Yi Qilin Yi Weilin Wang Weilin Wang Weilin Wang Lingling Wang Lingling Wang Lingling Wang Linsheng Song Linsheng Song |
| spellingShingle |
Conghui Liu Conghui Liu Conghui Liu Chuanyan Yang Chuanyan Yang Chuanyan Yang Mengqiang Wang Shuai Jiang Qilin Yi Qilin Yi Qilin Yi Weilin Wang Weilin Wang Weilin Wang Lingling Wang Lingling Wang Lingling Wang Linsheng Song Linsheng Song A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas Frontiers in Immunology tetraspanin phagosomes recruitment receptor innate immune response Crassostrea gigas |
| author_facet |
Conghui Liu Conghui Liu Conghui Liu Chuanyan Yang Chuanyan Yang Chuanyan Yang Mengqiang Wang Shuai Jiang Qilin Yi Qilin Yi Qilin Yi Weilin Wang Weilin Wang Weilin Wang Lingling Wang Lingling Wang Lingling Wang Linsheng Song Linsheng Song |
| author_sort |
Conghui Liu |
| title |
A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas |
| title_short |
A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas |
| title_full |
A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas |
| title_fullStr |
A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas |
| title_full_unstemmed |
A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas |
| title_sort |
cd63 homolog specially recruited to the fungi-contained phagosomes is involved in the cellular immune response of oyster crassostrea gigas |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Immunology |
| issn |
1664-3224 |
| publishDate |
2020-07-01 |
| description |
Cluster of differentiation 63 (CD63), a four-transmembrane glycoprotein in the subfamily of tetraspanin, has been widely recognized as a gateway from the infection of foreign invaders to the immune defense of hosts. Its role in Pacific oyster Crassostrea gigas is, however, yet to be discovered. This work makes contributions by identifying CgCD63H, a CD63 homolog with four transmembrane domains and one conservative CCG motif, and establishing its role as a receptor that participates in immune recognition and hemocyte phagocytosis. The presence of CgCD63H messenger RNA (mRNA) in hepatopancreas, labial palps, gill, and hemocytes is confirmed. The expression level of mRNA in hemocytes is found significantly (p < 0.01) upregulated after the injection of Vibrio splendidus. CgCD63H protein, typically distributed over the plasma membrane of oyster hemocytes, is recruited to the Yarrowia lipolytica-containing phagosomes after the stimulation of Y. lipolytica. The recombinant CgCD63H protein expresses binding capacity to glucan (GLU), peptidoglycan (PGN), and lipopolysaccharide (LPS) in the presence of lyophilized hemolymph. The phagocytic rate of hemocytes toward V. splendidus and Y. lipolytica is significantly inhibited (p < 0.01) after incubation with anti-CgCD63H antibody. Our work further suggests that CgCD63H functions as a receptor involved in the immune recognition and hemocyte phagocytosis against invading pathogen, which can be a marker candidate for the hemocyte typing in C. gigas. |
| topic |
tetraspanin phagosomes recruitment receptor innate immune response Crassostrea gigas |
| url |
https://www.frontiersin.org/article/10.3389/fimmu.2020.01379/full |
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doaj-ba846b904c004f5bbc3d422aa7cec7332020-11-25T03:06:47ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-07-011110.3389/fimmu.2020.01379539310A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigasConghui Liu0Conghui Liu1Conghui Liu2Chuanyan Yang3Chuanyan Yang4Chuanyan Yang5Mengqiang Wang6Shuai Jiang7Qilin Yi8Qilin Yi9Qilin Yi10Weilin Wang11Weilin Wang12Weilin Wang13Lingling Wang14Lingling Wang15Lingling Wang16Linsheng Song17Linsheng Song18Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, ChinaKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLiaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, ChinaLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, ChinaLiaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, ChinaDalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, ChinaKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, ChinaLiaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, ChinaDalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, ChinaKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLiaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, ChinaDalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, ChinaLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, ChinaLiaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, ChinaDalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, ChinaLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, ChinaLiaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, ChinaCluster of differentiation 63 (CD63), a four-transmembrane glycoprotein in the subfamily of tetraspanin, has been widely recognized as a gateway from the infection of foreign invaders to the immune defense of hosts. Its role in Pacific oyster Crassostrea gigas is, however, yet to be discovered. This work makes contributions by identifying CgCD63H, a CD63 homolog with four transmembrane domains and one conservative CCG motif, and establishing its role as a receptor that participates in immune recognition and hemocyte phagocytosis. The presence of CgCD63H messenger RNA (mRNA) in hepatopancreas, labial palps, gill, and hemocytes is confirmed. The expression level of mRNA in hemocytes is found significantly (p < 0.01) upregulated after the injection of Vibrio splendidus. CgCD63H protein, typically distributed over the plasma membrane of oyster hemocytes, is recruited to the Yarrowia lipolytica-containing phagosomes after the stimulation of Y. lipolytica. The recombinant CgCD63H protein expresses binding capacity to glucan (GLU), peptidoglycan (PGN), and lipopolysaccharide (LPS) in the presence of lyophilized hemolymph. The phagocytic rate of hemocytes toward V. splendidus and Y. lipolytica is significantly inhibited (p < 0.01) after incubation with anti-CgCD63H antibody. Our work further suggests that CgCD63H functions as a receptor involved in the immune recognition and hemocyte phagocytosis against invading pathogen, which can be a marker candidate for the hemocyte typing in C. gigas.https://www.frontiersin.org/article/10.3389/fimmu.2020.01379/fulltetraspaninphagosomes recruitmentreceptorinnate immune responseCrassostrea gigas |