Nasopharyngeal carriage of Streptococcus pneumoniae in Romanian children before the introduction of the pneumococcal conjugated vaccination into the national immunization programme: a national, multi-centre, cross-sectional observational study
Objectives: We analysed the distribution of vaccine and non-vaccine Streptococcus pneumoniae serotypes and the antimicrobial susceptibility of pneumococcal strains isolated from healthy Romanian children. Methods: A multi-centre cross-sectional study was performed in four counties to evaluate carri...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2014-12-01
|
Series: | International Journal of Infectious Diseases |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971214016385 |
id |
doaj-ba9e71839feb417e97d51574d16907df |
---|---|
record_format |
Article |
spelling |
doaj-ba9e71839feb417e97d51574d16907df2020-11-24T22:56:14ZengElsevierInternational Journal of Infectious Diseases1201-97121878-35112014-12-0129C16917310.1016/j.ijid.2014.08.020Nasopharyngeal carriage of Streptococcus pneumoniae in Romanian children before the introduction of the pneumococcal conjugated vaccination into the national immunization programme: a national, multi-centre, cross-sectional observational studyMonica Luminos0Olga Dorobat1George Jugulete2Gabriel Adrian Popescu3Dragos Florea4Anca Draganescu5Anca Streinu Cercel6Alexandru Rafila7National Institute for Infectious Diseases “Prof Dr Matei Bals”, Grozovici 1, 021105 Bucharest, RomaniaNational Institute for Infectious Diseases “Prof Dr Matei Bals”, Grozovici 1, 021105 Bucharest, RomaniaNational Institute for Infectious Diseases “Prof Dr Matei Bals”, Grozovici 1, 021105 Bucharest, RomaniaNational Institute for Infectious Diseases “Prof Dr Matei Bals”, Grozovici 1, 021105 Bucharest, RomaniaNational Institute for Infectious Diseases “Prof Dr Matei Bals”, Grozovici 1, 021105 Bucharest, RomaniaNational Institute for Infectious Diseases “Prof Dr Matei Bals”, Grozovici 1, 021105 Bucharest, RomaniaNational Institute for Infectious Diseases “Prof Dr Matei Bals”, Grozovici 1, 021105 Bucharest, RomaniaNational Institute for Infectious Diseases “Prof Dr Matei Bals”, Grozovici 1, 021105 Bucharest, Romania Objectives: We analysed the distribution of vaccine and non-vaccine Streptococcus pneumoniae serotypes and the antimicrobial susceptibility of pneumococcal strains isolated from healthy Romanian children. Methods: A multi-centre cross-sectional study was performed in four counties to evaluate carried strains of S. pneumoniae isolated from 2000 children aged 0–5 years. Results: S. pneumoniae carriage was detected in 25.25% of the tested children. Carriage increased from 16.7% among infants to 29.4% in 3–5-year-old children (p < 0.0001). The proportions of the serotypes included in pneumococcal conjugate vaccines PCV7, PCV10, and PCV13 among our isolates were 39.9%, 40.1%, and 58.7%, respectively. Erythromycin resistance was 72.5%, and it was significantly lower in non-vaccine serotypes compared with PCV13 serotypes: 57.3% versus 83.6% (p < 10−7). Penicillin minimum inhibitory concentrations (MICs) >0.064 mg/l were recorded in 71.6%, but the penicillin MIC was >2 mg/l for only 8.4% of tested isolates. Conclusions: In Romanian children, the majority of carried S. pneumoniae isolates are vaccine serotypes. The isolates with MICs defining macrolide resistance were very frequent, as well as the isolates with MICs defining penicillin resistance in the case of meningitis or penicillin dose-dependent susceptibility for other infections, mainly for the strains belonging to PCV13 serotypes. The implementation of PCV13 within the Romanian national immunization programme could reduce the circulation of these strains with higher macrolide and/or penicillin MICs. http://www.sciencedirect.com/science/article/pii/S1201971214016385Pneumococcal conjugated vaccine serotypesMacrolide resistancePenicillin MICStreptococcus pneumoniae carriage |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Monica Luminos Olga Dorobat George Jugulete Gabriel Adrian Popescu Dragos Florea Anca Draganescu Anca Streinu Cercel Alexandru Rafila |
spellingShingle |
Monica Luminos Olga Dorobat George Jugulete Gabriel Adrian Popescu Dragos Florea Anca Draganescu Anca Streinu Cercel Alexandru Rafila Nasopharyngeal carriage of Streptococcus pneumoniae in Romanian children before the introduction of the pneumococcal conjugated vaccination into the national immunization programme: a national, multi-centre, cross-sectional observational study International Journal of Infectious Diseases Pneumococcal conjugated vaccine serotypes Macrolide resistance Penicillin MIC Streptococcus pneumoniae carriage |
author_facet |
Monica Luminos Olga Dorobat George Jugulete Gabriel Adrian Popescu Dragos Florea Anca Draganescu Anca Streinu Cercel Alexandru Rafila |
author_sort |
Monica Luminos |
title |
Nasopharyngeal carriage of Streptococcus pneumoniae in Romanian children before the introduction of the pneumococcal conjugated vaccination into the national immunization programme: a national, multi-centre, cross-sectional observational study |
title_short |
Nasopharyngeal carriage of Streptococcus pneumoniae in Romanian children before the introduction of the pneumococcal conjugated vaccination into the national immunization programme: a national, multi-centre, cross-sectional observational study |
title_full |
Nasopharyngeal carriage of Streptococcus pneumoniae in Romanian children before the introduction of the pneumococcal conjugated vaccination into the national immunization programme: a national, multi-centre, cross-sectional observational study |
title_fullStr |
Nasopharyngeal carriage of Streptococcus pneumoniae in Romanian children before the introduction of the pneumococcal conjugated vaccination into the national immunization programme: a national, multi-centre, cross-sectional observational study |
title_full_unstemmed |
Nasopharyngeal carriage of Streptococcus pneumoniae in Romanian children before the introduction of the pneumococcal conjugated vaccination into the national immunization programme: a national, multi-centre, cross-sectional observational study |
title_sort |
nasopharyngeal carriage of streptococcus pneumoniae in romanian children before the introduction of the pneumococcal conjugated vaccination into the national immunization programme: a national, multi-centre, cross-sectional observational study |
publisher |
Elsevier |
series |
International Journal of Infectious Diseases |
issn |
1201-9712 1878-3511 |
publishDate |
2014-12-01 |
description |
Objectives: We analysed the distribution of vaccine and non-vaccine Streptococcus pneumoniae serotypes and the antimicrobial susceptibility of pneumococcal strains isolated from healthy Romanian children.
Methods: A multi-centre cross-sectional study was performed in four counties to evaluate carried strains of S. pneumoniae isolated from 2000 children aged 0–5 years.
Results: S. pneumoniae carriage was detected in 25.25% of the tested children. Carriage increased from 16.7% among infants to 29.4% in 3–5-year-old children (p < 0.0001). The proportions of the serotypes included in pneumococcal conjugate vaccines PCV7, PCV10, and PCV13 among our isolates were 39.9%, 40.1%, and 58.7%, respectively. Erythromycin resistance was 72.5%, and it was significantly lower in non-vaccine serotypes compared with PCV13 serotypes: 57.3% versus 83.6% (p < 10−7). Penicillin minimum inhibitory concentrations (MICs) >0.064 mg/l were recorded in 71.6%, but the penicillin MIC was >2 mg/l for only 8.4% of tested isolates.
Conclusions: In Romanian children, the majority of carried S. pneumoniae isolates are vaccine serotypes. The isolates with MICs defining macrolide resistance were very frequent, as well as the isolates with MICs defining penicillin resistance in the case of meningitis or penicillin dose-dependent susceptibility for other infections, mainly for the strains belonging to PCV13 serotypes. The implementation of PCV13 within the Romanian national immunization programme could reduce the circulation of these strains with higher macrolide and/or penicillin MICs.
|
topic |
Pneumococcal conjugated vaccine serotypes Macrolide resistance Penicillin MIC Streptococcus pneumoniae carriage |
url |
http://www.sciencedirect.com/science/article/pii/S1201971214016385 |
work_keys_str_mv |
AT monicaluminos nasopharyngealcarriageofstreptococcuspneumoniaeinromanianchildrenbeforetheintroductionofthepneumococcalconjugatedvaccinationintothenationalimmunizationprogrammeanationalmulticentrecrosssectionalobservationalstudy AT olgadorobat nasopharyngealcarriageofstreptococcuspneumoniaeinromanianchildrenbeforetheintroductionofthepneumococcalconjugatedvaccinationintothenationalimmunizationprogrammeanationalmulticentrecrosssectionalobservationalstudy AT georgejugulete nasopharyngealcarriageofstreptococcuspneumoniaeinromanianchildrenbeforetheintroductionofthepneumococcalconjugatedvaccinationintothenationalimmunizationprogrammeanationalmulticentrecrosssectionalobservationalstudy AT gabrieladrianpopescu nasopharyngealcarriageofstreptococcuspneumoniaeinromanianchildrenbeforetheintroductionofthepneumococcalconjugatedvaccinationintothenationalimmunizationprogrammeanationalmulticentrecrosssectionalobservationalstudy AT dragosflorea nasopharyngealcarriageofstreptococcuspneumoniaeinromanianchildrenbeforetheintroductionofthepneumococcalconjugatedvaccinationintothenationalimmunizationprogrammeanationalmulticentrecrosssectionalobservationalstudy AT ancadraganescu nasopharyngealcarriageofstreptococcuspneumoniaeinromanianchildrenbeforetheintroductionofthepneumococcalconjugatedvaccinationintothenationalimmunizationprogrammeanationalmulticentrecrosssectionalobservationalstudy AT ancastreinucercel nasopharyngealcarriageofstreptococcuspneumoniaeinromanianchildrenbeforetheintroductionofthepneumococcalconjugatedvaccinationintothenationalimmunizationprogrammeanationalmulticentrecrosssectionalobservationalstudy AT alexandrurafila nasopharyngealcarriageofstreptococcuspneumoniaeinromanianchildrenbeforetheintroductionofthepneumococcalconjugatedvaccinationintothenationalimmunizationprogrammeanationalmulticentrecrosssectionalobservationalstudy |
_version_ |
1725654187835916288 |