miRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class Insecta
Abstract Aedes aegypti L. is the most important vector of arboviruses such as dengue, Zika, chikungunya, Mayaro, and yellow fever, which impact millions of people’s health per year. MicroRNA profile has been described in some mosquito species as being important for biological processes such as diges...
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doaj-baa54f0576fd428789b87f9c6af8d9592021-05-23T11:33:16ZengNature Publishing GroupScientific Reports2045-23222021-05-0111111110.1038/s41598-021-90095-9miRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class InsectaIram Pablo Rodríguez-Sanchez0Daniel Rafael Saldaña-Torres1Olga Karina Villanueva-Segura2Maria Lourdes Garza-Rodriguez3Mayra A. Gómez-Govea4Ghongwei Liang5María de Lourdes Ramírez-Ahuja6Margarita De La Luz Martinez-Fierro7Ivan Delgado-Enciso8Laura E. Martinez-de-Villarreal9Yu Zhou10Adriana E. Flores-Suarez11Xi Chen12Diana Resendez-Pérez13Chen-Yu Zhang14Gustavo Ponce-Garcia15Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Biologicas, Laboratorio de Fisiología Estructural y MolecularUniversidad Autonoma de Nuevo Leon, Facultad de Ciencias Biologicas, Laboratorio de Fisiología Estructural y MolecularUniversidad Autonoma de Nuevo Leon, Facultad de Ciencias Biologicas, Laboratorio de Fisiología Estructural y MolecularUniversidad Autónoma de Nuevo León, Hospital Universitario “Dr. José Eleuterio González”, Centro Universitario Contra el Cáncer.Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Biologicas, Laboratorio de Fisiología Estructural y MolecularState Key Laboratory of Pharmaceutical Biotechnology, NJU Advanced Institute of Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, Nanjing UniversityUniversidad Autonoma de Nuevo Leon, Facultad de Ciencias Biologicas, Laboratorio de Fisiología Estructural y MolecularLaboratorio de Medicina Molecular, Universidad Autonoma de Zacatecas, Unidad Academica de Medicina HumanaFacultad de Medicina, Universidad de ColimaUniversidad Autonoma de Nuevo Leon, Facultad de Medicina, Departamento de GenéticaState Key Laboratory of Pharmaceutical Biotechnology, NJU Advanced Institute of Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, Nanjing UniversityUniversidad Autonoma de Nuevo Leon, Facultad de Ciencias Biologicas, Departamento de Zoologia de Invertebrados, Laboratorio de Entomologia MedicaState Key Laboratory of Pharmaceutical Biotechnology, NJU Advanced Institute of Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, Nanjing UniversityUniversidad Autonoma de Nuevo Leon, Facultad de Ciencias Biologicas, Departamento de Biología Celular y GenéticaState Key Laboratory of Pharmaceutical Biotechnology, NJU Advanced Institute of Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, Nanjing UniversityUniversidad Autonoma de Nuevo Leon, Facultad de Ciencias Biologicas, Departamento de Zoologia de Invertebrados, Laboratorio de Entomologia MedicaAbstract Aedes aegypti L. is the most important vector of arboviruses such as dengue, Zika, chikungunya, Mayaro, and yellow fever, which impact millions of people’s health per year. MicroRNA profile has been described in some mosquito species as being important for biological processes such as digestion of blood, oviposition, sexual differentiation, insecticide resistance, and pathogens dissemination. We identified the miRNAs of Ae. aegypti females, males and eggs of a reference insecticide susceptible strain New Orleans and compared them with those other insects to determine miRNA fingerprint by new-generation sequencing. The sequences were analyzed using data mining tools and categorization, followed by differential expression analysis and conservation with other insects. A total of 55 conserved miRNAs were identified, of which 34 were of holometabolous insects and 21 shared with hemimetabolous insects. Of these miRNAs, 32 had differential expression within the stages analyzed. Three predominant functions of miRNA were related to embryonic development regulation, metamorphosis, and basal functions. The findings of this research describe new information on Ae. aegypti physiology which could be useful for the development of new control strategies, particularly in mosquito development and metamorphosis processes.https://doi.org/10.1038/s41598-021-90095-9 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Iram Pablo Rodríguez-Sanchez Daniel Rafael Saldaña-Torres Olga Karina Villanueva-Segura Maria Lourdes Garza-Rodriguez Mayra A. Gómez-Govea Ghongwei Liang María de Lourdes Ramírez-Ahuja Margarita De La Luz Martinez-Fierro Ivan Delgado-Enciso Laura E. Martinez-de-Villarreal Yu Zhou Adriana E. Flores-Suarez Xi Chen Diana Resendez-Pérez Chen-Yu Zhang Gustavo Ponce-Garcia |
spellingShingle |
Iram Pablo Rodríguez-Sanchez Daniel Rafael Saldaña-Torres Olga Karina Villanueva-Segura Maria Lourdes Garza-Rodriguez Mayra A. Gómez-Govea Ghongwei Liang María de Lourdes Ramírez-Ahuja Margarita De La Luz Martinez-Fierro Ivan Delgado-Enciso Laura E. Martinez-de-Villarreal Yu Zhou Adriana E. Flores-Suarez Xi Chen Diana Resendez-Pérez Chen-Yu Zhang Gustavo Ponce-Garcia miRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class Insecta Scientific Reports |
author_facet |
Iram Pablo Rodríguez-Sanchez Daniel Rafael Saldaña-Torres Olga Karina Villanueva-Segura Maria Lourdes Garza-Rodriguez Mayra A. Gómez-Govea Ghongwei Liang María de Lourdes Ramírez-Ahuja Margarita De La Luz Martinez-Fierro Ivan Delgado-Enciso Laura E. Martinez-de-Villarreal Yu Zhou Adriana E. Flores-Suarez Xi Chen Diana Resendez-Pérez Chen-Yu Zhang Gustavo Ponce-Garcia |
author_sort |
Iram Pablo Rodríguez-Sanchez |
title |
miRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class Insecta |
title_short |
miRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class Insecta |
title_full |
miRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class Insecta |
title_fullStr |
miRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class Insecta |
title_full_unstemmed |
miRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class Insecta |
title_sort |
mirnas of aedes aegypti (linnaeus 1762) conserved in six orders of the class insecta |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-05-01 |
description |
Abstract Aedes aegypti L. is the most important vector of arboviruses such as dengue, Zika, chikungunya, Mayaro, and yellow fever, which impact millions of people’s health per year. MicroRNA profile has been described in some mosquito species as being important for biological processes such as digestion of blood, oviposition, sexual differentiation, insecticide resistance, and pathogens dissemination. We identified the miRNAs of Ae. aegypti females, males and eggs of a reference insecticide susceptible strain New Orleans and compared them with those other insects to determine miRNA fingerprint by new-generation sequencing. The sequences were analyzed using data mining tools and categorization, followed by differential expression analysis and conservation with other insects. A total of 55 conserved miRNAs were identified, of which 34 were of holometabolous insects and 21 shared with hemimetabolous insects. Of these miRNAs, 32 had differential expression within the stages analyzed. Three predominant functions of miRNA were related to embryonic development regulation, metamorphosis, and basal functions. The findings of this research describe new information on Ae. aegypti physiology which could be useful for the development of new control strategies, particularly in mosquito development and metamorphosis processes. |
url |
https://doi.org/10.1038/s41598-021-90095-9 |
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