Exhaled Breath Reflects Prolonged Exercise and Statin Use during a Field Campaign
Volatile organic compounds (VOCs) in exhaled breath provide insights into various metabolic processes and can be used to monitor physiological response to exercise and medication. We integrated and validated in situ a sampling and analysis protocol using proton transfer reaction time-of-flight mass...
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MDPI AG
2021-03-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/4/192 |
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doaj-baadd2c9c57c407bbcaab7810d351ef22021-03-25T00:04:58ZengMDPI AGMetabolites2218-19892021-03-011119219210.3390/metabo11040192Exhaled Breath Reflects Prolonged Exercise and Statin Use during a Field CampaignBen Henderson0Guilherme Lopes Batista1Carlo G. Bertinetto2Joris Meurs3Dušan Materić4Coen C. W. G. Bongers5Neeltje A. E. Allard6Thijs M. H. Eijsvogels7Rupert Holzinger8Frans J. M. Harren9Jeroen J. Jansen10Maria T. E. Hopman11Simona M. Cristescu12Department of Molecular and Laser Physics, Institute for Molecules and Materials, Radboud University, 6525 XZ Nijmegen, The NetherlandsDepartment of Molecular and Laser Physics, Institute for Molecules and Materials, Radboud University, 6525 XZ Nijmegen, The NetherlandsDepartment of Analytical Chemistry and Chemometrics, Institute for Molecules and Materials, Radboud University, 6525 XZ Nijmegen, The NetherlandsDepartment of Molecular and Laser Physics, Institute for Molecules and Materials, Radboud University, 6525 XZ Nijmegen, The NetherlandsDepartment of Molecular and Laser Physics, Institute for Molecules and Materials, Radboud University, 6525 XZ Nijmegen, The NetherlandsDepartment of Physiology, Radboud Institute for Health Sciences, Radboud University Medical Center, 6525 XZ Nijmegen, The NetherlandsDepartment of Physiology, Radboud Institute for Health Sciences, Radboud University Medical Center, 6525 XZ Nijmegen, The NetherlandsDepartment of Physiology, Radboud Institute for Health Sciences, Radboud University Medical Center, 6525 XZ Nijmegen, The NetherlandsInstitute for Marine and Atmospheric Research, Utrecht University, 3584 CC Utrecht, The NetherlandsDepartment of Molecular and Laser Physics, Institute for Molecules and Materials, Radboud University, 6525 XZ Nijmegen, The NetherlandsDepartment of Analytical Chemistry and Chemometrics, Institute for Molecules and Materials, Radboud University, 6525 XZ Nijmegen, The NetherlandsDepartment of Physiology, Radboud Institute for Health Sciences, Radboud University Medical Center, 6525 XZ Nijmegen, The NetherlandsDepartment of Molecular and Laser Physics, Institute for Molecules and Materials, Radboud University, 6525 XZ Nijmegen, The NetherlandsVolatile organic compounds (VOCs) in exhaled breath provide insights into various metabolic processes and can be used to monitor physiological response to exercise and medication. We integrated and validated in situ a sampling and analysis protocol using proton transfer reaction time-of-flight mass spectrometry (PTR-ToF-MS) for exhaled breath research. The approach was demonstrated on a participant cohort comprising users of the cholesterol-lowering drug statins and non-statin users during a field campaign of three days of prolonged and repeated exercise, with no restrictions on food or drink consumption. The effect of prolonged exercise was reflected in the exhaled breath of participants, and relevant VOCs were identified. Most of the VOCs, such as acetone, showed an increase in concentration after the first day of walking and subsequent decrease towards baseline levels prior to walking on the second day. A cluster of short-chain fatty acids including acetic acid, butanoic acid, and propionic acid were identified in exhaled breath as potential indicators of gut microbiota activity relating to exercise and drug use. We have provided novel information regarding the use of breathomics for non-invasive monitoring of changes in human metabolism and especially for the gut microbiome activity in relation to exercise and the use of medication, such as statins.https://www.mdpi.com/2218-1989/11/4/192prolonged exercisebreathshort-chain fatty acidsstatinPTR-ToF-MS |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Ben Henderson Guilherme Lopes Batista Carlo G. Bertinetto Joris Meurs Dušan Materić Coen C. W. G. Bongers Neeltje A. E. Allard Thijs M. H. Eijsvogels Rupert Holzinger Frans J. M. Harren Jeroen J. Jansen Maria T. E. Hopman Simona M. Cristescu |
| spellingShingle |
Ben Henderson Guilherme Lopes Batista Carlo G. Bertinetto Joris Meurs Dušan Materić Coen C. W. G. Bongers Neeltje A. E. Allard Thijs M. H. Eijsvogels Rupert Holzinger Frans J. M. Harren Jeroen J. Jansen Maria T. E. Hopman Simona M. Cristescu Exhaled Breath Reflects Prolonged Exercise and Statin Use during a Field Campaign Metabolites prolonged exercise breath short-chain fatty acids statin PTR-ToF-MS |
| author_facet |
Ben Henderson Guilherme Lopes Batista Carlo G. Bertinetto Joris Meurs Dušan Materić Coen C. W. G. Bongers Neeltje A. E. Allard Thijs M. H. Eijsvogels Rupert Holzinger Frans J. M. Harren Jeroen J. Jansen Maria T. E. Hopman Simona M. Cristescu |
| author_sort |
Ben Henderson |
| title |
Exhaled Breath Reflects Prolonged Exercise and Statin Use during a Field Campaign |
| title_short |
Exhaled Breath Reflects Prolonged Exercise and Statin Use during a Field Campaign |
| title_full |
Exhaled Breath Reflects Prolonged Exercise and Statin Use during a Field Campaign |
| title_fullStr |
Exhaled Breath Reflects Prolonged Exercise and Statin Use during a Field Campaign |
| title_full_unstemmed |
Exhaled Breath Reflects Prolonged Exercise and Statin Use during a Field Campaign |
| title_sort |
exhaled breath reflects prolonged exercise and statin use during a field campaign |
| publisher |
MDPI AG |
| series |
Metabolites |
| issn |
2218-1989 |
| publishDate |
2021-03-01 |
| description |
Volatile organic compounds (VOCs) in exhaled breath provide insights into various metabolic processes and can be used to monitor physiological response to exercise and medication. We integrated and validated in situ a sampling and analysis protocol using proton transfer reaction time-of-flight mass spectrometry (PTR-ToF-MS) for exhaled breath research. The approach was demonstrated on a participant cohort comprising users of the cholesterol-lowering drug statins and non-statin users during a field campaign of three days of prolonged and repeated exercise, with no restrictions on food or drink consumption. The effect of prolonged exercise was reflected in the exhaled breath of participants, and relevant VOCs were identified. Most of the VOCs, such as acetone, showed an increase in concentration after the first day of walking and subsequent decrease towards baseline levels prior to walking on the second day. A cluster of short-chain fatty acids including acetic acid, butanoic acid, and propionic acid were identified in exhaled breath as potential indicators of gut microbiota activity relating to exercise and drug use. We have provided novel information regarding the use of breathomics for non-invasive monitoring of changes in human metabolism and especially for the gut microbiome activity in relation to exercise and the use of medication, such as statins. |
| topic |
prolonged exercise breath short-chain fatty acids statin PTR-ToF-MS |
| url |
https://www.mdpi.com/2218-1989/11/4/192 |
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