Automated systems in the identification and determination of methicillin resistance among coagulase negative staphylococci
Coagulase-negative staphylococci (CoNS) are an important cause of nosocomial bacteremia, specially in patients with indwelling devices or those submitted to invasive medical procedures. The identification of species and the accurate and rapid detection of methicillin resistance are directly dependen...
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Instituto Oswaldo Cruz, Ministério da Saúde
2006-05-01
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doaj-bab38168ae744ccfa2cac850098173492020-11-25T00:33:52ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.0074-02761678-80602006-05-01101327728010.1590/S0074-02762006000300009Automated systems in the identification and determination of methicillin resistance among coagulase negative staphylococciJuliana CaierãoSilvana SupertiCícero AG DiasPedro Alves d'AzevedoCoagulase-negative staphylococci (CoNS) are an important cause of nosocomial bacteremia, specially in patients with indwelling devices or those submitted to invasive medical procedures. The identification of species and the accurate and rapid detection of methicillin resistance are directly dependent on the quality of the identification and susceptibility tests used, either manual or automated. The objective of this study was to evaluate the accuracy of two automated systems MicroScan and Vitek - in the identification of CoNS species and determination of susceptibility to methicillin, considering as gold standard the biochemical tests and the characterization of the mecA gene by polymerase chain reaction, respectively. MicroScan presented better results in the identification of CoNS species (accuracy of 96.8 vs 78.8%, respectively); isolates from the following species had no precise identification: Staphylococcus haemolyticus, S. simulans, and S. capitis. Both systems were similar in the characterization of methicillin resistance. The higher discrepancies for gene mec detection were observed among species other than S. epidermidis (S. hominis, S. saprophyticus, S. sciuri, S. haemolyticus, S. warneri, S. cohnii), and those with borderline MICs.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000300009coagulase-negative staphylococcimethicillinautomated systems |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Juliana Caierão Silvana Superti Cícero AG Dias Pedro Alves d'Azevedo |
spellingShingle |
Juliana Caierão Silvana Superti Cícero AG Dias Pedro Alves d'Azevedo Automated systems in the identification and determination of methicillin resistance among coagulase negative staphylococci Memórias do Instituto Oswaldo Cruz. coagulase-negative staphylococci methicillin automated systems |
author_facet |
Juliana Caierão Silvana Superti Cícero AG Dias Pedro Alves d'Azevedo |
author_sort |
Juliana Caierão |
title |
Automated systems in the identification and determination of methicillin resistance among coagulase negative staphylococci |
title_short |
Automated systems in the identification and determination of methicillin resistance among coagulase negative staphylococci |
title_full |
Automated systems in the identification and determination of methicillin resistance among coagulase negative staphylococci |
title_fullStr |
Automated systems in the identification and determination of methicillin resistance among coagulase negative staphylococci |
title_full_unstemmed |
Automated systems in the identification and determination of methicillin resistance among coagulase negative staphylococci |
title_sort |
automated systems in the identification and determination of methicillin resistance among coagulase negative staphylococci |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
series |
Memórias do Instituto Oswaldo Cruz. |
issn |
0074-0276 1678-8060 |
publishDate |
2006-05-01 |
description |
Coagulase-negative staphylococci (CoNS) are an important cause of nosocomial bacteremia, specially in patients with indwelling devices or those submitted to invasive medical procedures. The identification of species and the accurate and rapid detection of methicillin resistance are directly dependent on the quality of the identification and susceptibility tests used, either manual or automated. The objective of this study was to evaluate the accuracy of two automated systems MicroScan and Vitek - in the identification of CoNS species and determination of susceptibility to methicillin, considering as gold standard the biochemical tests and the characterization of the mecA gene by polymerase chain reaction, respectively. MicroScan presented better results in the identification of CoNS species (accuracy of 96.8 vs 78.8%, respectively); isolates from the following species had no precise identification: Staphylococcus haemolyticus, S. simulans, and S. capitis. Both systems were similar in the characterization of methicillin resistance. The higher discrepancies for gene mec detection were observed among species other than S. epidermidis (S. hominis, S. saprophyticus, S. sciuri, S. haemolyticus, S. warneri, S. cohnii), and those with borderline MICs. |
topic |
coagulase-negative staphylococci methicillin automated systems |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000300009 |
work_keys_str_mv |
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