Convenient Agarose Preparation with Hydrogen Peroxide and Desulfation Process Analysis
Agarose is a natural seaweed polysaccharide and widely used in the medicine, food, and biological fields because of its high gel strength, non-toxicity, and electrical neutrality. The sulfate group is one of the main charged groups that affect the performance of agarose. In the present study, a simp...
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doaj-bb00eb8ddae0438396a72ce32601673e2021-06-01T00:50:57ZengMDPI AGMarine Drugs1660-33972021-05-011929729710.3390/md19060297Convenient Agarose Preparation with Hydrogen Peroxide and Desulfation Process AnalysisCong Zhang0Ding An1Qiong Xiao2Fu-Quan Chen3Yong-Hui Zhang4Hui-Fen Weng5An-Feng Xiao6College of Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaCollege of Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaCollege of Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaCollege of Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaCollege of Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaCollege of Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaCollege of Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaAgarose is a natural seaweed polysaccharide and widely used in the medicine, food, and biological fields because of its high gel strength, non-toxicity, and electrical neutrality. The sulfate group is one of the main charged groups that affect the performance of agarose. In the present study, a simple, eco-friendly, and efficient method was explored for agarose preparation. After desulfation with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), the sulfate content of agar reached 0.21%. Together with gel strength, electroendosmosis, gelling and melting temperature, the indicators of desulfated agar met the standards of commercially available agarose. Notably, the desulfated agar can be used as an agarose gel electrophoresis medium to separate DNA molecules, and the separation effect is as good as that of commercially available agarose. Further, the H<sub>2</sub>O<sub>2</sub> desulfation process was analyzed. The addition of a hydroxyl radical (HO•) scavenger remarkably decreased the H<sub>2</sub>O<sub>2</sub> desulfation rate, indicating that HO• has a certain role in agar desulfation. Sulfate content detection indicated that sulfur was removed from agar molecules in the form of sulfate ions (SO<sub>4</sub><sup>2−</sup>) and metal sulfate. The band absence at 850 cm<sup>−1</sup> indicated that the sulfate groups at C-4 of D-galactose in sulfated galactan were eliminated.https://www.mdpi.com/1660-3397/19/6/297agarosehydrogen peroxidedesulfation analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cong Zhang Ding An Qiong Xiao Fu-Quan Chen Yong-Hui Zhang Hui-Fen Weng An-Feng Xiao |
spellingShingle |
Cong Zhang Ding An Qiong Xiao Fu-Quan Chen Yong-Hui Zhang Hui-Fen Weng An-Feng Xiao Convenient Agarose Preparation with Hydrogen Peroxide and Desulfation Process Analysis Marine Drugs agarose hydrogen peroxide desulfation analysis |
author_facet |
Cong Zhang Ding An Qiong Xiao Fu-Quan Chen Yong-Hui Zhang Hui-Fen Weng An-Feng Xiao |
author_sort |
Cong Zhang |
title |
Convenient Agarose Preparation with Hydrogen Peroxide and Desulfation Process Analysis |
title_short |
Convenient Agarose Preparation with Hydrogen Peroxide and Desulfation Process Analysis |
title_full |
Convenient Agarose Preparation with Hydrogen Peroxide and Desulfation Process Analysis |
title_fullStr |
Convenient Agarose Preparation with Hydrogen Peroxide and Desulfation Process Analysis |
title_full_unstemmed |
Convenient Agarose Preparation with Hydrogen Peroxide and Desulfation Process Analysis |
title_sort |
convenient agarose preparation with hydrogen peroxide and desulfation process analysis |
publisher |
MDPI AG |
series |
Marine Drugs |
issn |
1660-3397 |
publishDate |
2021-05-01 |
description |
Agarose is a natural seaweed polysaccharide and widely used in the medicine, food, and biological fields because of its high gel strength, non-toxicity, and electrical neutrality. The sulfate group is one of the main charged groups that affect the performance of agarose. In the present study, a simple, eco-friendly, and efficient method was explored for agarose preparation. After desulfation with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), the sulfate content of agar reached 0.21%. Together with gel strength, electroendosmosis, gelling and melting temperature, the indicators of desulfated agar met the standards of commercially available agarose. Notably, the desulfated agar can be used as an agarose gel electrophoresis medium to separate DNA molecules, and the separation effect is as good as that of commercially available agarose. Further, the H<sub>2</sub>O<sub>2</sub> desulfation process was analyzed. The addition of a hydroxyl radical (HO•) scavenger remarkably decreased the H<sub>2</sub>O<sub>2</sub> desulfation rate, indicating that HO• has a certain role in agar desulfation. Sulfate content detection indicated that sulfur was removed from agar molecules in the form of sulfate ions (SO<sub>4</sub><sup>2−</sup>) and metal sulfate. The band absence at 850 cm<sup>−1</sup> indicated that the sulfate groups at C-4 of D-galactose in sulfated galactan were eliminated. |
topic |
agarose hydrogen peroxide desulfation analysis |
url |
https://www.mdpi.com/1660-3397/19/6/297 |
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