The rice diacylglycerol kinase family: functional analysis using transient RNA interference
Diacylglycerol kinase (DGK) is a pivotal enzyme that phosphorylates diacylglycerol (DAG) to form phosphatidic acid (PA). The production of PA from phospholipase D (PLD) and the coupled phospholipase C (PLC)/DGK route is an important signaling process in animal and plant cells. In this study, we repo...
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doaj-bb23404aaf614ee19aa72b1521bd27862020-11-25T00:12:34ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2012-03-01310.3389/fpls.2012.0006021372The rice diacylglycerol kinase family: functional analysis using transient RNA interferenceHongliang eGe0Chu eChen1Wen eJing2Qun eZhang3Hong eWang4Rong eWang5Wenhua eZhang6Nanjing Agricultural UniversityNanjing Agricultural UniversityNanjing Agricultural UniversityNanjing Agricultural UniversityNanjing Agricultural UniversityNanjing Agricultural UniversityNanjing Agricultural UniversityDiacylglycerol kinase (DGK) is a pivotal enzyme that phosphorylates diacylglycerol (DAG) to form phosphatidic acid (PA). The production of PA from phospholipase D (PLD) and the coupled phospholipase C (PLC)/DGK route is an important signaling process in animal and plant cells. In this study, we report a genomic analysis of eight putative rice DGKs encoded by a gene family (OsDGKs) grouped into three clusters. To further investigate the functions of the OsDGKs, a double-stranded RNA (dsRNA)-induced RNA silencing method was established. Introduction of in vitro-synthesized dsRNAs corresponding to a unique or conserved region of OsDGKs into rice protoplasts abolished or diminished the expression of individual or multiple OsDGK genes. Suppressing the expression of OsDGKs resulted in a distinct depletion of the transcripts of the defense gene OsNPR1 and the salt-responsive gene OsCIPK15. Our primary results suggest that OsDGKs are involved in the signaling of stress responses.http://journal.frontiersin.org/Journal/10.3389/fpls.2012.00060/fullDiacylglycerol KinaseRNA Interferencedouble-stranded RNArice |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hongliang eGe Chu eChen Wen eJing Qun eZhang Hong eWang Rong eWang Wenhua eZhang |
spellingShingle |
Hongliang eGe Chu eChen Wen eJing Qun eZhang Hong eWang Rong eWang Wenhua eZhang The rice diacylglycerol kinase family: functional analysis using transient RNA interference Frontiers in Plant Science Diacylglycerol Kinase RNA Interference double-stranded RNA rice |
author_facet |
Hongliang eGe Chu eChen Wen eJing Qun eZhang Hong eWang Rong eWang Wenhua eZhang |
author_sort |
Hongliang eGe |
title |
The rice diacylglycerol kinase family: functional analysis using transient RNA interference |
title_short |
The rice diacylglycerol kinase family: functional analysis using transient RNA interference |
title_full |
The rice diacylglycerol kinase family: functional analysis using transient RNA interference |
title_fullStr |
The rice diacylglycerol kinase family: functional analysis using transient RNA interference |
title_full_unstemmed |
The rice diacylglycerol kinase family: functional analysis using transient RNA interference |
title_sort |
rice diacylglycerol kinase family: functional analysis using transient rna interference |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2012-03-01 |
description |
Diacylglycerol kinase (DGK) is a pivotal enzyme that phosphorylates diacylglycerol (DAG) to form phosphatidic acid (PA). The production of PA from phospholipase D (PLD) and the coupled phospholipase C (PLC)/DGK route is an important signaling process in animal and plant cells. In this study, we report a genomic analysis of eight putative rice DGKs encoded by a gene family (OsDGKs) grouped into three clusters. To further investigate the functions of the OsDGKs, a double-stranded RNA (dsRNA)-induced RNA silencing method was established. Introduction of in vitro-synthesized dsRNAs corresponding to a unique or conserved region of OsDGKs into rice protoplasts abolished or diminished the expression of individual or multiple OsDGK genes. Suppressing the expression of OsDGKs resulted in a distinct depletion of the transcripts of the defense gene OsNPR1 and the salt-responsive gene OsCIPK15. Our primary results suggest that OsDGKs are involved in the signaling of stress responses. |
topic |
Diacylglycerol Kinase RNA Interference double-stranded RNA rice |
url |
http://journal.frontiersin.org/Journal/10.3389/fpls.2012.00060/full |
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