Unraveling the Spatiotemporal Human Pluripotency in Embryonic Development
There have been significant advances in understanding human embryogenesis using human pluripotent stem cells (hPSCs) in conventional monolayer and 3D self-organized cultures. Thus, in vitro models have contributed to elucidate the molecular mechanisms for specification and differentiation during dev...
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doaj-bb31fdf90e0147dfbc5a777302a1a45c2021-06-23T06:35:28ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-06-01910.3389/fcell.2021.676998676998Unraveling the Spatiotemporal Human Pluripotency in Embryonic DevelopmentDaniela Ávila-González0Daniela Ávila-González1Wendy Portillo2Guadalupe García-López3Anayansi Molina-Hernández4Néstor E. Díaz-Martínez5Néstor F. Díaz6Instituto de Neurobiología, Universidad Nacional Autónoma de México, Juriquilla, MexicoInstituto Nacional de Perinatología, Mexico City, MexicoInstituto de Neurobiología, Universidad Nacional Autónoma de México, Juriquilla, MexicoInstituto Nacional de Perinatología, Mexico City, MexicoInstituto Nacional de Perinatología, Mexico City, MexicoBiotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Guadalajara, MexicoInstituto Nacional de Perinatología, Mexico City, MexicoThere have been significant advances in understanding human embryogenesis using human pluripotent stem cells (hPSCs) in conventional monolayer and 3D self-organized cultures. Thus, in vitro models have contributed to elucidate the molecular mechanisms for specification and differentiation during development. However, the molecular and functional spectrum of human pluripotency (i.e., intermediate states, pluripotency subtypes and regionalization) is still not fully understood. This review describes the mechanisms that establish and maintain pluripotency in human embryos and their differences with mouse embryos. Further, it describes a new pluripotent state representing a transition between naïve and primed pluripotency. This review also presents the data that divide pluripotency into substates expressing epiblast regionalization and amnion specification as well as primordial germ cells in primates. Finally, this work analyzes the amnion’s relevance as an “signaling center” for regionalization before the onset of gastrulation.https://www.frontiersin.org/articles/10.3389/fcell.2021.676998/fullnaïveprimedamnionformativesynthetic biology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Daniela Ávila-González Daniela Ávila-González Wendy Portillo Guadalupe García-López Anayansi Molina-Hernández Néstor E. Díaz-Martínez Néstor F. Díaz |
spellingShingle |
Daniela Ávila-González Daniela Ávila-González Wendy Portillo Guadalupe García-López Anayansi Molina-Hernández Néstor E. Díaz-Martínez Néstor F. Díaz Unraveling the Spatiotemporal Human Pluripotency in Embryonic Development Frontiers in Cell and Developmental Biology naïve primed amnion formative synthetic biology |
author_facet |
Daniela Ávila-González Daniela Ávila-González Wendy Portillo Guadalupe García-López Anayansi Molina-Hernández Néstor E. Díaz-Martínez Néstor F. Díaz |
author_sort |
Daniela Ávila-González |
title |
Unraveling the Spatiotemporal Human Pluripotency in Embryonic Development |
title_short |
Unraveling the Spatiotemporal Human Pluripotency in Embryonic Development |
title_full |
Unraveling the Spatiotemporal Human Pluripotency in Embryonic Development |
title_fullStr |
Unraveling the Spatiotemporal Human Pluripotency in Embryonic Development |
title_full_unstemmed |
Unraveling the Spatiotemporal Human Pluripotency in Embryonic Development |
title_sort |
unraveling the spatiotemporal human pluripotency in embryonic development |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cell and Developmental Biology |
issn |
2296-634X |
publishDate |
2021-06-01 |
description |
There have been significant advances in understanding human embryogenesis using human pluripotent stem cells (hPSCs) in conventional monolayer and 3D self-organized cultures. Thus, in vitro models have contributed to elucidate the molecular mechanisms for specification and differentiation during development. However, the molecular and functional spectrum of human pluripotency (i.e., intermediate states, pluripotency subtypes and regionalization) is still not fully understood. This review describes the mechanisms that establish and maintain pluripotency in human embryos and their differences with mouse embryos. Further, it describes a new pluripotent state representing a transition between naïve and primed pluripotency. This review also presents the data that divide pluripotency into substates expressing epiblast regionalization and amnion specification as well as primordial germ cells in primates. Finally, this work analyzes the amnion’s relevance as an “signaling center” for regionalization before the onset of gastrulation. |
topic |
naïve primed amnion formative synthetic biology |
url |
https://www.frontiersin.org/articles/10.3389/fcell.2021.676998/full |
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