A Review on Macroscale and Microscale Cell Lysis Methods
The lysis of cells in order to extract the nucleic acids or proteins inside it is a crucial unit operation in biomolecular analysis. This paper presents a critical evaluation of the various methods that are available both in the macro and micro scale for cell lysis. Various types of cells, the struc...
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MDPI AG
2017-03-01
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| Series: | Micromachines |
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| Online Access: | http://www.mdpi.com/2072-666X/8/3/83 |
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doaj-bb38edae5a6d4d13bb617b372b03f7be2020-11-24T23:08:01ZengMDPI AGMicromachines2072-666X2017-03-01838310.3390/mi8030083mi8030083A Review on Macroscale and Microscale Cell Lysis MethodsMohammed Shehadul Islam0Aditya Aryasomayajula1Ponnambalam Ravi Selvaganapathy2Department of Mechanical Engineering, McMaster University, Hamilton, ON L8S 4L7, CanadaDepartment of Mechanical Engineering, McMaster University, Hamilton, ON L8S 4L7, CanadaDepartment of Mechanical Engineering, McMaster University, Hamilton, ON L8S 4L7, CanadaThe lysis of cells in order to extract the nucleic acids or proteins inside it is a crucial unit operation in biomolecular analysis. This paper presents a critical evaluation of the various methods that are available both in the macro and micro scale for cell lysis. Various types of cells, the structure of their membranes are discussed initially. Then, various methods that are currently used to lyse cells in the macroscale are discussed and compared. Subsequently, popular methods for micro scale cell lysis and different microfluidic devices used are detailed with their advantages and disadvantages. Finally, a comparison of different techniques used in microfluidics platform has been presented which will be helpful to select method for a particular application.http://www.mdpi.com/2072-666X/8/3/83cell lysiscell lysis methodsmicrofluidicselectrical lysismechanical lysisthermal lysis |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Mohammed Shehadul Islam Aditya Aryasomayajula Ponnambalam Ravi Selvaganapathy |
| spellingShingle |
Mohammed Shehadul Islam Aditya Aryasomayajula Ponnambalam Ravi Selvaganapathy A Review on Macroscale and Microscale Cell Lysis Methods Micromachines cell lysis cell lysis methods microfluidics electrical lysis mechanical lysis thermal lysis |
| author_facet |
Mohammed Shehadul Islam Aditya Aryasomayajula Ponnambalam Ravi Selvaganapathy |
| author_sort |
Mohammed Shehadul Islam |
| title |
A Review on Macroscale and Microscale Cell Lysis Methods |
| title_short |
A Review on Macroscale and Microscale Cell Lysis Methods |
| title_full |
A Review on Macroscale and Microscale Cell Lysis Methods |
| title_fullStr |
A Review on Macroscale and Microscale Cell Lysis Methods |
| title_full_unstemmed |
A Review on Macroscale and Microscale Cell Lysis Methods |
| title_sort |
review on macroscale and microscale cell lysis methods |
| publisher |
MDPI AG |
| series |
Micromachines |
| issn |
2072-666X |
| publishDate |
2017-03-01 |
| description |
The lysis of cells in order to extract the nucleic acids or proteins inside it is a crucial unit operation in biomolecular analysis. This paper presents a critical evaluation of the various methods that are available both in the macro and micro scale for cell lysis. Various types of cells, the structure of their membranes are discussed initially. Then, various methods that are currently used to lyse cells in the macroscale are discussed and compared. Subsequently, popular methods for micro scale cell lysis and different microfluidic devices used are detailed with their advantages and disadvantages. Finally, a comparison of different techniques used in microfluidics platform has been presented which will be helpful to select method for a particular application. |
| topic |
cell lysis cell lysis methods microfluidics electrical lysis mechanical lysis thermal lysis |
| url |
http://www.mdpi.com/2072-666X/8/3/83 |
| work_keys_str_mv |
AT mohammedshehadulislam areviewonmacroscaleandmicroscalecelllysismethods AT adityaaryasomayajula areviewonmacroscaleandmicroscalecelllysismethods AT ponnambalamraviselvaganapathy areviewonmacroscaleandmicroscalecelllysismethods AT mohammedshehadulislam reviewonmacroscaleandmicroscalecelllysismethods AT adityaaryasomayajula reviewonmacroscaleandmicroscalecelllysismethods AT ponnambalamraviselvaganapathy reviewonmacroscaleandmicroscalecelllysismethods |
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1725615808212631552 |