Optimization of DNA Extraction from Individual Sand Flies for PCR Amplification

Optimization of DNA Extraction from Individual Sand Flies for PCR Amplification

Numerous protocols have been published for extracting DNA from phlebotomines. Nevertheless, their small size is generally an issue in terms of yield, efficiency, and purity, for large-scale individual sand fly DNA extractions when using traditional methods. Even though this can be circumvented with...

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Bibliographic Details
Main Authors: Lorena G. Caligiuri, Adolfo E. Sandoval, Jose C. Miranda, Felipe A. Pessoa, María S. Santini, Oscar D. Salomón, Nagila F. C. Secundino, Christina B. McCarthy
Format: Article
Language:English
Published: MDPI AG 2019-05-01
Series:Methods and Protocols
Subjects:
sand fly
DNA extraction
calcium
PCR
lysis buffer
<i>Lutzomyia</i>
Online Access:https://www.mdpi.com/2409-9279/2/2/36

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