The Elucidation of Total Polyphenols, Individual Phenolic Compounds, Antioxidant Activity of Three Underutilized Fruit Species—Black Crowberry, Honeyberry, European Cranberry with Their Accumulation
Total polyphenols content (TPC), antioxidant activity (AA) and polyphenolic spectrum assay of three underutilized fruit species—black crowberry (<i>Empetrum nigrum</i>), honeyberry (<i>Lonicera kamtschatica</i>) and European cranberry (<i>Vaccinium oxycoccos)</i>...
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MDPI AG
2021-12-01
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English |
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Article |
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DOAJ |
author |
Tünde Juríková Jiří Mlček Štefan Balla Monika Ondrášová Libor Dokoupil Jiří Sochor L’uba Ďurišová Pavol Eliáš Anna Adámková Mojmír Baroň Sezai Ercisli |
spellingShingle |
Tünde Juríková Jiří Mlček Štefan Balla Monika Ondrášová Libor Dokoupil Jiří Sochor L’uba Ďurišová Pavol Eliáš Anna Adámková Mojmír Baroň Sezai Ercisli The Elucidation of Total Polyphenols, Individual Phenolic Compounds, Antioxidant Activity of Three Underutilized Fruit Species—Black Crowberry, Honeyberry, European Cranberry with Their Accumulation Agronomy <i>Empetrum nigrum</i> <i>Lonicera kamtchatica</i> a <i>Vaccinium oxycoccus</i> fruits total phenolic content antioxidant activity |
author_facet |
Tünde Juríková Jiří Mlček Štefan Balla Monika Ondrášová Libor Dokoupil Jiří Sochor L’uba Ďurišová Pavol Eliáš Anna Adámková Mojmír Baroň Sezai Ercisli |
author_sort |
Tünde Juríková |
title |
The Elucidation of Total Polyphenols, Individual Phenolic Compounds, Antioxidant Activity of Three Underutilized Fruit Species—Black Crowberry, Honeyberry, European Cranberry with Their Accumulation |
title_short |
The Elucidation of Total Polyphenols, Individual Phenolic Compounds, Antioxidant Activity of Three Underutilized Fruit Species—Black Crowberry, Honeyberry, European Cranberry with Their Accumulation |
title_full |
The Elucidation of Total Polyphenols, Individual Phenolic Compounds, Antioxidant Activity of Three Underutilized Fruit Species—Black Crowberry, Honeyberry, European Cranberry with Their Accumulation |
title_fullStr |
The Elucidation of Total Polyphenols, Individual Phenolic Compounds, Antioxidant Activity of Three Underutilized Fruit Species—Black Crowberry, Honeyberry, European Cranberry with Their Accumulation |
title_full_unstemmed |
The Elucidation of Total Polyphenols, Individual Phenolic Compounds, Antioxidant Activity of Three Underutilized Fruit Species—Black Crowberry, Honeyberry, European Cranberry with Their Accumulation |
title_sort |
elucidation of total polyphenols, individual phenolic compounds, antioxidant activity of three underutilized fruit species—black crowberry, honeyberry, european cranberry with their accumulation |
publisher |
MDPI AG |
series |
Agronomy |
issn |
2073-4395 |
publishDate |
2021-12-01 |
description |
Total polyphenols content (TPC), antioxidant activity (AA) and polyphenolic spectrum assay of three underutilized fruit species—black crowberry (<i>Empetrum nigrum</i>), honeyberry (<i>Lonicera kamtschatica</i>) and European cranberry (<i>Vaccinium oxycoccos)</i> is the aim of the present work. TPC and AA assay was performed by spectrophotometry and “individual phenolic compounds” were established by high-performance liquid chromatography. The results showed that TPC ranged from 1.61 ± 0.16 (European cranberry) up to 5.65 ± 0.01 (Black cowberry) gallic acid mg/g fresh weight. The highest value of DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay was determined in black crowberry fruit (7.43 ± 0.34 mmol TROLOX/g fresh weight of fruit). In all samples, the most prevalent phenolic acid was ferulic acid with the highest content in the samples of black crowberry (77.73 ± 3.99 µg/g FW of fruit), black crowberry and honeyberry were present by the highest level of gallic acid (21.82 ± 1.53; 15.07 ± 2.45 µg/g FW of fruit). Honeyberry represented a valuable source of quercetin (12.18 ± 7.88 µg/g FW of fruit), whereas European cranberry and honeyberry are a source of rutin (28.48 ± 0.83 and 27.99 ± 1.78 µg/g FW of fruit, respectively). The results of statistical analyses proved significant differences between cowberry and European cranberry in TPC content and in AA among assayed species. In the same way, statistically significant differences were confirmed in phenolic acids among the assayed species except for European cranberry-honeyberry (in trans p-coumaric, protocatequic and cinnamic acid) and honeyberry-cowberry (in cinnamic acid content). The catechin content is significantly influenced by species (<i>p</i> ≤ 0.05), on the other hand the species has no influence on the resveratrol content (<i>p</i> > 0.05). Each of the studied species can be considered as valuable source of total polyphenols and individual phenolic compounds. Polyphenolic compounds were most frequently accumulated in the vacuole as well as in the outer layers of pericarp. |
topic |
<i>Empetrum nigrum</i> <i>Lonicera kamtchatica</i> a <i>Vaccinium oxycoccus</i> fruits total phenolic content antioxidant activity |
url |
https://www.mdpi.com/2073-4395/11/1/73 |
work_keys_str_mv |
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doaj-bb4210fd7d194f9c81bf4588626984252021-04-02T18:31:06ZengMDPI AGAgronomy2073-43952021-12-0111737310.3390/agronomy11010073The Elucidation of Total Polyphenols, Individual Phenolic Compounds, Antioxidant Activity of Three Underutilized Fruit Species—Black Crowberry, Honeyberry, European Cranberry with Their AccumulationTünde Juríková0Jiří Mlček1Štefan Balla2Monika Ondrášová3Libor Dokoupil4Jiří Sochor5L’uba Ďurišová6Pavol Eliáš7Anna Adámková8Mojmír Baroň9Sezai Ercisli10Institute for Teacher Training, Faculty of Central European Studies, Constantine the Philosopher University in Nitra, Dražovská 4, 949 74 Nitra, SlovakiaDepartment of Food Analysis and Chemistry, Faculty of Technology, Tomas Bata University in Zlín, Vavreckova 275, 760 01 Zlín, Czech RepublicInstitute for Teacher Training, Faculty of Central European Studies, Constantine the Philosopher University in Nitra, Dražovská 4, 949 74 Nitra, SlovakiaDepartment of Food Analysis and Chemistry, Faculty of Technology, Tomas Bata University in Zlín, Vavreckova 275, 760 01 Zlín, Czech RepublicDepartment of Breeding and Propagation of Horticultural Plants, Faculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech RepublicDepartment of Viticulture and Enology, Faculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech RepublicDepartment of Environment and Biology, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, A. Hlinku 2, 949 76 Nitra, SlovakiaDepartment of Environment and Biology, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, A. Hlinku 2, 949 76 Nitra, SlovakiaDepartment of Food Analysis and Chemistry, Faculty of Technology, Tomas Bata University in Zlín, Vavreckova 275, 760 01 Zlín, Czech RepublicDepartment of Viticulture and Enology, Faculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech RepublicDepartment of Horticulture, Agricultural Faculty, Ataturk University, 25240 Erzurum, TurkeyTotal polyphenols content (TPC), antioxidant activity (AA) and polyphenolic spectrum assay of three underutilized fruit species—black crowberry (<i>Empetrum nigrum</i>), honeyberry (<i>Lonicera kamtschatica</i>) and European cranberry (<i>Vaccinium oxycoccos)</i> is the aim of the present work. TPC and AA assay was performed by spectrophotometry and “individual phenolic compounds” were established by high-performance liquid chromatography. The results showed that TPC ranged from 1.61 ± 0.16 (European cranberry) up to 5.65 ± 0.01 (Black cowberry) gallic acid mg/g fresh weight. The highest value of DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay was determined in black crowberry fruit (7.43 ± 0.34 mmol TROLOX/g fresh weight of fruit). In all samples, the most prevalent phenolic acid was ferulic acid with the highest content in the samples of black crowberry (77.73 ± 3.99 µg/g FW of fruit), black crowberry and honeyberry were present by the highest level of gallic acid (21.82 ± 1.53; 15.07 ± 2.45 µg/g FW of fruit). Honeyberry represented a valuable source of quercetin (12.18 ± 7.88 µg/g FW of fruit), whereas European cranberry and honeyberry are a source of rutin (28.48 ± 0.83 and 27.99 ± 1.78 µg/g FW of fruit, respectively). The results of statistical analyses proved significant differences between cowberry and European cranberry in TPC content and in AA among assayed species. In the same way, statistically significant differences were confirmed in phenolic acids among the assayed species except for European cranberry-honeyberry (in trans p-coumaric, protocatequic and cinnamic acid) and honeyberry-cowberry (in cinnamic acid content). The catechin content is significantly influenced by species (<i>p</i> ≤ 0.05), on the other hand the species has no influence on the resveratrol content (<i>p</i> > 0.05). Each of the studied species can be considered as valuable source of total polyphenols and individual phenolic compounds. Polyphenolic compounds were most frequently accumulated in the vacuole as well as in the outer layers of pericarp.https://www.mdpi.com/2073-4395/11/1/73<i>Empetrum nigrum</i><i>Lonicera kamtchatica</i> a <i>Vaccinium oxycoccus</i>fruitstotal phenolic contentantioxidant activity |