MicroRNA-378 regulates adiponectin expression in adipose tissue: a new plausible mechanism.
AIMS: Mechanisms regulating adiponectin expression have not been fully clarified. MicroRNAs (miRNAs), small non-coding RNAs that regulate gene expression, are involved in biological processes, including obesity and insulin resistance. We evaluated whether the miRNA-378 pathway is involved in regulat...
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doaj-bb43af5afa8e4d1383281bb2685f09de2020-11-25T02:50:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11153710.1371/journal.pone.0111537MicroRNA-378 regulates adiponectin expression in adipose tissue: a new plausible mechanism.Masayoshi IshidaMichio ShimabukuroShusuke YagiSachiko NishimotoChisayo KozukaDaiju FukudaTakeshi SoekiHiroaki MasuzakiMasato TsutsuiMasataka SataAIMS: Mechanisms regulating adiponectin expression have not been fully clarified. MicroRNAs (miRNAs), small non-coding RNAs that regulate gene expression, are involved in biological processes, including obesity and insulin resistance. We evaluated whether the miRNA-378 pathway is involved in regulating adiponectin expression. METHODS AND RESULTS: First, we determined a putative target site for miRNA-378 in the 3 prime untranslated region (3'UTR) of the adiponectin gene by in silico analysis. The levels of adiponectin mRNA and protein were decreased in 3T3-L1 cells overexpressing the mimic of miRNA-378. Luminescence activity in HEK293T cells expressing a renilla-luciferase-adiponectin-3'UTR sequence was inhibited by overexpressing the mimic of miRNA-378, and the decrease was reversed by adding the inhibitor of miRNA-378. Moreover, we confirmed the inhibitory effects of the mimic were cancelled in a deleted mutant of the miR-378 3'-UTR binding site. Addition of tumor necrosis factor-α (TNFα) led a upregulation of miR-378 and downregulation of adiponectin at mRNA and protein levels in 3T3-L1 cells. Level of miR-378 was higher and mRNA level of adiponectin was lower in diabetic ob/ob mice than those of normal C57BL/6 mice and levels of miR378 and adiponectin were negatively well correlated (r = -0.624, p = 0.004). CONCLUSIONS: We found that levels of miRNA-378 could modulate adiponectin expression via the 3'UTR sequence-binding site. Our findings warrant further investigations into the role of miRNAs in regulating the adiponectin expression.http://europepmc.org/articles/PMC4224402?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Masayoshi Ishida Michio Shimabukuro Shusuke Yagi Sachiko Nishimoto Chisayo Kozuka Daiju Fukuda Takeshi Soeki Hiroaki Masuzaki Masato Tsutsui Masataka Sata |
spellingShingle |
Masayoshi Ishida Michio Shimabukuro Shusuke Yagi Sachiko Nishimoto Chisayo Kozuka Daiju Fukuda Takeshi Soeki Hiroaki Masuzaki Masato Tsutsui Masataka Sata MicroRNA-378 regulates adiponectin expression in adipose tissue: a new plausible mechanism. PLoS ONE |
author_facet |
Masayoshi Ishida Michio Shimabukuro Shusuke Yagi Sachiko Nishimoto Chisayo Kozuka Daiju Fukuda Takeshi Soeki Hiroaki Masuzaki Masato Tsutsui Masataka Sata |
author_sort |
Masayoshi Ishida |
title |
MicroRNA-378 regulates adiponectin expression in adipose tissue: a new plausible mechanism. |
title_short |
MicroRNA-378 regulates adiponectin expression in adipose tissue: a new plausible mechanism. |
title_full |
MicroRNA-378 regulates adiponectin expression in adipose tissue: a new plausible mechanism. |
title_fullStr |
MicroRNA-378 regulates adiponectin expression in adipose tissue: a new plausible mechanism. |
title_full_unstemmed |
MicroRNA-378 regulates adiponectin expression in adipose tissue: a new plausible mechanism. |
title_sort |
microrna-378 regulates adiponectin expression in adipose tissue: a new plausible mechanism. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
AIMS: Mechanisms regulating adiponectin expression have not been fully clarified. MicroRNAs (miRNAs), small non-coding RNAs that regulate gene expression, are involved in biological processes, including obesity and insulin resistance. We evaluated whether the miRNA-378 pathway is involved in regulating adiponectin expression. METHODS AND RESULTS: First, we determined a putative target site for miRNA-378 in the 3 prime untranslated region (3'UTR) of the adiponectin gene by in silico analysis. The levels of adiponectin mRNA and protein were decreased in 3T3-L1 cells overexpressing the mimic of miRNA-378. Luminescence activity in HEK293T cells expressing a renilla-luciferase-adiponectin-3'UTR sequence was inhibited by overexpressing the mimic of miRNA-378, and the decrease was reversed by adding the inhibitor of miRNA-378. Moreover, we confirmed the inhibitory effects of the mimic were cancelled in a deleted mutant of the miR-378 3'-UTR binding site. Addition of tumor necrosis factor-α (TNFα) led a upregulation of miR-378 and downregulation of adiponectin at mRNA and protein levels in 3T3-L1 cells. Level of miR-378 was higher and mRNA level of adiponectin was lower in diabetic ob/ob mice than those of normal C57BL/6 mice and levels of miR378 and adiponectin were negatively well correlated (r = -0.624, p = 0.004). CONCLUSIONS: We found that levels of miRNA-378 could modulate adiponectin expression via the 3'UTR sequence-binding site. Our findings warrant further investigations into the role of miRNAs in regulating the adiponectin expression. |
url |
http://europepmc.org/articles/PMC4224402?pdf=render |
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