Facial vein injection of human cells in severe combined immunodeficiency (SCID) neonatal mice

Facial vein injection of human cells in severe combined immunodeficiency (SCID) neonatal mice

Intravenous injection is a standard procedure for delivering human stem cells and therapeutic agents. Currently, genetically modified severe combined immunodeficiency (SCID) mice are used for engraftment studies using human cells. SCID neonates have better integration and survivability of human cell...

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Main Authors: Nimshitha Pavathuparambil Abdul Manaph, Mohammed Al-Hawwas, Liang Liu, Donghui Liu, John Hayball, Xin-Fu Zhou
Format: Article
Language:English
Published: Elsevier 2018-01-01
Series:MethodsX
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016118301626
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spelling doaj-bb467a34c67946739c95486f3908b4102020-11-25T01:36:17ZengElsevierMethodsX2215-01612018-01-01512811286Facial vein injection of human cells in severe combined immunodeficiency (SCID) neonatal miceNimshitha Pavathuparambil Abdul Manaph0Mohammed Al-Hawwas1Liang Liu2Donghui Liu3John Hayball4Xin-Fu Zhou5School of Pharmacy and Medical Sciences, Sansom Institute, University of South Australia, Adelaide 5000, South Australia, Australia; Central Northern Adelaide Renal and Transplantation Service, Royal Adelaide Hospital, Adelaide 5000, South Australia, Australia; School of Medicine, Faculty of Health Sciences, University of Adelaide, Adelaide 5000, South Australia, AustraliaSchool of Pharmacy and Medical Sciences, Sansom Institute, University of South Australia, Adelaide 5000, South Australia, AustraliaSchool of Pharmacy and Medical Sciences, Sansom Institute, University of South Australia, Adelaide 5000, South Australia, AustraliaSchool of Pharmacy and Medical Sciences, Sansom Institute, University of South Australia, Adelaide 5000, South Australia, Australia; Jiangsu Key Laboratory of Drug Screening, China Pharmaceutical University, Nanjing, ChinaSchool of Pharmacy and Medical Sciences, Sansom Institute, University of South Australia, Adelaide 5000, South Australia, AustraliaSchool of Pharmacy and Medical Sciences, Sansom Institute, University of South Australia, Adelaide 5000, South Australia, Australia; Corresponding author.Intravenous injection is a standard procedure for delivering human stem cells and therapeutic agents. Currently, genetically modified severe combined immunodeficiency (SCID) mice are used for engraftment studies using human cells. SCID neonates have better integration and survivability of human cells compared to adult SCID mice, as their immune system will not be developed in the first few days after birth. However, intravenous injections in neonates are difficult. This protocol describes a reliable and reproducible method for injecting cells into the facial vein of P3/P4 (3 or 4 days post-birth) SCID neonates to study their engraftment. The injection was safe and well tolerated by the pups. Post-injection analysis revealed the distribution of tagged cells in different organs. Results suggest that this new method can serve as a pre-analysis for transplantation studies using human stem cells before in vivo animal model testing. Method name: Facial vein injection in P3/P4 pups, Keywords: Neonates, Facial vein, Intravenous injection, Stem cells, Deliveryhttp://www.sciencedirect.com/science/article/pii/S2215016118301626
collection DOAJ
language English
format Article
sources DOAJ
author Nimshitha Pavathuparambil Abdul Manaph
Mohammed Al-Hawwas
Liang Liu
Donghui Liu
John Hayball
Xin-Fu Zhou
spellingShingle Nimshitha Pavathuparambil Abdul Manaph
Mohammed Al-Hawwas
Liang Liu
Donghui Liu
John Hayball
Xin-Fu Zhou
Facial vein injection of human cells in severe combined immunodeficiency (SCID) neonatal mice
MethodsX
author_facet Nimshitha Pavathuparambil Abdul Manaph
Mohammed Al-Hawwas
Liang Liu
Donghui Liu
John Hayball
Xin-Fu Zhou
author_sort Nimshitha Pavathuparambil Abdul Manaph
title Facial vein injection of human cells in severe combined immunodeficiency (SCID) neonatal mice
title_short Facial vein injection of human cells in severe combined immunodeficiency (SCID) neonatal mice
title_full Facial vein injection of human cells in severe combined immunodeficiency (SCID) neonatal mice
title_fullStr Facial vein injection of human cells in severe combined immunodeficiency (SCID) neonatal mice
title_full_unstemmed Facial vein injection of human cells in severe combined immunodeficiency (SCID) neonatal mice
title_sort facial vein injection of human cells in severe combined immunodeficiency (scid) neonatal mice
publisher Elsevier
series MethodsX
issn 2215-0161
publishDate 2018-01-01
description Intravenous injection is a standard procedure for delivering human stem cells and therapeutic agents. Currently, genetically modified severe combined immunodeficiency (SCID) mice are used for engraftment studies using human cells. SCID neonates have better integration and survivability of human cells compared to adult SCID mice, as their immune system will not be developed in the first few days after birth. However, intravenous injections in neonates are difficult. This protocol describes a reliable and reproducible method for injecting cells into the facial vein of P3/P4 (3 or 4 days post-birth) SCID neonates to study their engraftment. The injection was safe and well tolerated by the pups. Post-injection analysis revealed the distribution of tagged cells in different organs. Results suggest that this new method can serve as a pre-analysis for transplantation studies using human stem cells before in vivo animal model testing. Method name: Facial vein injection in P3/P4 pups, Keywords: Neonates, Facial vein, Intravenous injection, Stem cells, Delivery
url http://www.sciencedirect.com/science/article/pii/S2215016118301626
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AT mohammedalhawwas facialveininjectionofhumancellsinseverecombinedimmunodeficiencyscidneonatalmice
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