CAM: A quality control pipeline for MNase-seq data.
Nucleosome organization affects the accessibility of cis-elements to trans-acting factors. Micrococcal nuclease digestion followed by high-throughput sequencing (MNase-seq) is the most popular technology used to profile nucleosome organization on a genome-wide scale. Evaluating the data quality of M...
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doaj-bb4e4221e6b44744834c8597d35c7f292020-11-24T21:34:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01128e018277110.1371/journal.pone.0182771CAM: A quality control pipeline for MNase-seq data.Sheng'en HuXiaolan ChenJi LiaoYiqing ChenChengchen ZhaoYong ZhangNucleosome organization affects the accessibility of cis-elements to trans-acting factors. Micrococcal nuclease digestion followed by high-throughput sequencing (MNase-seq) is the most popular technology used to profile nucleosome organization on a genome-wide scale. Evaluating the data quality of MNase-seq data remains challenging, especially in mammalian. There is a strong need for a convenient and comprehensive approach to obtain dedicated quality control (QC) for MNase-seq data analysis. Here we developed CAM, which is a comprehensive QC pipeline for MNase-seq data. The CAM pipeline provides multiple informative QC measurements and nucleosome organization profiles on different potentially functional regions for given MNase-seq data. CAM also includes 268 historical MNase-seq datasets from human and mouse as a reference atlas for unbiased assessment. CAM is freely available at: http://www.tongji.edu.cn/~zhanglab/CAM.http://europepmc.org/articles/PMC5546596?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sheng'en Hu Xiaolan Chen Ji Liao Yiqing Chen Chengchen Zhao Yong Zhang |
spellingShingle |
Sheng'en Hu Xiaolan Chen Ji Liao Yiqing Chen Chengchen Zhao Yong Zhang CAM: A quality control pipeline for MNase-seq data. PLoS ONE |
author_facet |
Sheng'en Hu Xiaolan Chen Ji Liao Yiqing Chen Chengchen Zhao Yong Zhang |
author_sort |
Sheng'en Hu |
title |
CAM: A quality control pipeline for MNase-seq data. |
title_short |
CAM: A quality control pipeline for MNase-seq data. |
title_full |
CAM: A quality control pipeline for MNase-seq data. |
title_fullStr |
CAM: A quality control pipeline for MNase-seq data. |
title_full_unstemmed |
CAM: A quality control pipeline for MNase-seq data. |
title_sort |
cam: a quality control pipeline for mnase-seq data. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2017-01-01 |
description |
Nucleosome organization affects the accessibility of cis-elements to trans-acting factors. Micrococcal nuclease digestion followed by high-throughput sequencing (MNase-seq) is the most popular technology used to profile nucleosome organization on a genome-wide scale. Evaluating the data quality of MNase-seq data remains challenging, especially in mammalian. There is a strong need for a convenient and comprehensive approach to obtain dedicated quality control (QC) for MNase-seq data analysis. Here we developed CAM, which is a comprehensive QC pipeline for MNase-seq data. The CAM pipeline provides multiple informative QC measurements and nucleosome organization profiles on different potentially functional regions for given MNase-seq data. CAM also includes 268 historical MNase-seq datasets from human and mouse as a reference atlas for unbiased assessment. CAM is freely available at: http://www.tongji.edu.cn/~zhanglab/CAM. |
url |
http://europepmc.org/articles/PMC5546596?pdf=render |
work_keys_str_mv |
AT shengenhu camaqualitycontrolpipelineformnaseseqdata AT xiaolanchen camaqualitycontrolpipelineformnaseseqdata AT jiliao camaqualitycontrolpipelineformnaseseqdata AT yiqingchen camaqualitycontrolpipelineformnaseseqdata AT chengchenzhao camaqualitycontrolpipelineformnaseseqdata AT yongzhang camaqualitycontrolpipelineformnaseseqdata |
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