Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples.

Trypanosoma cruzi has been classified into six Discrete Typing Units (DTUs), designated as TcI-TcVI. In order to effectively use this standardized nomenclature, a reproducible genotyping strategy is imperative. Several typing schemes have been developed with variable levels of complexity, selectivit...

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Main Authors: Carolina I Cura, Tomas Duffy, Raúl H Lucero, Margarita Bisio, Julie Péneau, Matilde Jimenez-Coello, Eva Calabuig, María J Gimenez, Edward Valencia Ayala, Sonia A Kjos, José Santalla, Susan M Mahaney, Nelly M Cayo, Claudia Nagel, Laura Barcán, Edith S Málaga Machaca, Karla Y Acosta Viana, Laurent Brutus, Susana B Ocampo, Christine Aznar, Cesar A Cuba Cuba, Ricardo E Gürtler, Janine M Ramsey, Isabela Ribeiro, John L VandeBerg, Zaida E Yadon, Antonio Osuna, Alejandro G Schijman
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-05-01
Series:PLoS Neglected Tropical Diseases
Online Access:http://europepmc.org/articles/PMC4437652?pdf=render