Effective Alu repeat based RT-Qpcr normalization in cancer cell perturbation experiments.

Effective Alu repeat based RT-Qpcr normalization in cancer cell perturbation experiments.

BACKGROUND: Measuring messenger RNA (mRNA) levels using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) is common practice in many laboratories. A specific set of mRNAs as internal control reference genes is considered as the preferred strategy to normalize RT-qPCR data. P...

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Main Authors: Ali Rihani, Tom Van Maerken, Filip Pattyn, Gert Van Peer, Anneleen Beckers, Sara De Brouwer, Candy Kumps, Evelien Mets, Joni Van der Meulen, Pieter Rondou, Carina Leonelli, Pieter Mestdagh, Frank Speleman, Jo Vandesompele
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3743747?pdf=render

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