Diversity of Pectin Rhamnogalacturonan I Rhamnosyltransferases in Glycosyltransferase Family 106
Rhamnogalacturonan I (RG-I) comprises approximately one quarter of the pectin molecules in land plants, and the backbone of RG-I consists of a repeating sequence of [2)-α-L-Rha(1-4)-α-D-GalUA(1-] disaccharide. Four Arabidopsis thaliana genes encoding RG-I rhamnosyltransferases (AtRRT1 to AtRRT4), wh...
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doaj-bbba1cbcc6ec4f65adc4bc5c3e9342da2020-11-25T03:44:29ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-07-011110.3389/fpls.2020.00997549866Diversity of Pectin Rhamnogalacturonan I Rhamnosyltransferases in Glycosyltransferase Family 106Bussarin Wachananawat0Takeshi Kuroha1Yuto Takenaka2Yuto Takenaka3Hiroyuki Kajiura4Satoshi Naramoto5Ryusuke Yokoyama6Kimitsune Ishizaki7Kazuhiko Nishitani8Takeshi Ishimizu9Takeshi Ishimizu10College of Life Sciences, Ritsumeikan University, Kusatsu, JapanGraduate School of Life Sciences, Tohoku University, Sendai, JapanCollege of Life Sciences, Ritsumeikan University, Kusatsu, JapanRitsumeikan Global Innovation Research Organization, Ritsumeikan University, Kusatsu, JapanCollege of Life Sciences, Ritsumeikan University, Kusatsu, JapanFaculty of Science, Hokkaido University, Sapporo, JapanGraduate School of Life Sciences, Tohoku University, Sendai, JapanGraduate School of Science, Kobe University, Kobe, JapanFaculty of Science, Kanagawa University, Hiratsuka, JapanCollege of Life Sciences, Ritsumeikan University, Kusatsu, JapanRitsumeikan Global Innovation Research Organization, Ritsumeikan University, Kusatsu, JapanRhamnogalacturonan I (RG-I) comprises approximately one quarter of the pectin molecules in land plants, and the backbone of RG-I consists of a repeating sequence of [2)-α-L-Rha(1-4)-α-D-GalUA(1-] disaccharide. Four Arabidopsis thaliana genes encoding RG-I rhamnosyltransferases (AtRRT1 to AtRRT4), which synthesize the disaccharide repeats, have been identified in the glycosyltransferase family (GT106). However, the functional role of RG-I in plant cell walls and the evolutional history of RRTs remains to be clarified. Here, we characterized the sole ortholog of AtRRT1–AtRRT4 in liverwort, Marchantia polymorpha, namely, MpRRT1. MpRRT1 had RRT activity and genetically complemented the AtRRT1-deficient mutant phenotype in A. thaliana. However, the MpRRT1-deficient M. polymorpha mutants showed no prominent morphological changes and only an approximate 20% reduction in rhamnose content in the cell wall fraction compared to that in wild-type plants, suggesting the existence of other RRT gene(s) in the M. polymorpha genome. As expected, we detected RRT activities in other GT106 family proteins such as those encoded by MpRRT3 in M. polymorpha and FRB1/AtRRT8 in A. thaliana, the deficient mutant of which affects cell adhesion. Our results show that RRT genes are more redundant and diverse in GT106 than previously thought.https://www.frontiersin.org/article/10.3389/fpls.2020.00997/fullglycosyltransferaseGT106Marchantia polymorphapectinrhamnogalacturonan Irhamnosyltransferase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bussarin Wachananawat Takeshi Kuroha Yuto Takenaka Yuto Takenaka Hiroyuki Kajiura Satoshi Naramoto Ryusuke Yokoyama Kimitsune Ishizaki Kazuhiko Nishitani Takeshi Ishimizu Takeshi Ishimizu |
spellingShingle |
Bussarin Wachananawat Takeshi Kuroha Yuto Takenaka Yuto Takenaka Hiroyuki Kajiura Satoshi Naramoto Ryusuke Yokoyama Kimitsune Ishizaki Kazuhiko Nishitani Takeshi Ishimizu Takeshi Ishimizu Diversity of Pectin Rhamnogalacturonan I Rhamnosyltransferases in Glycosyltransferase Family 106 Frontiers in Plant Science glycosyltransferase GT106 Marchantia polymorpha pectin rhamnogalacturonan I rhamnosyltransferase |
author_facet |
Bussarin Wachananawat Takeshi Kuroha Yuto Takenaka Yuto Takenaka Hiroyuki Kajiura Satoshi Naramoto Ryusuke Yokoyama Kimitsune Ishizaki Kazuhiko Nishitani Takeshi Ishimizu Takeshi Ishimizu |
author_sort |
Bussarin Wachananawat |
title |
Diversity of Pectin Rhamnogalacturonan I Rhamnosyltransferases in Glycosyltransferase Family 106 |
title_short |
Diversity of Pectin Rhamnogalacturonan I Rhamnosyltransferases in Glycosyltransferase Family 106 |
title_full |
Diversity of Pectin Rhamnogalacturonan I Rhamnosyltransferases in Glycosyltransferase Family 106 |
title_fullStr |
Diversity of Pectin Rhamnogalacturonan I Rhamnosyltransferases in Glycosyltransferase Family 106 |
title_full_unstemmed |
Diversity of Pectin Rhamnogalacturonan I Rhamnosyltransferases in Glycosyltransferase Family 106 |
title_sort |
diversity of pectin rhamnogalacturonan i rhamnosyltransferases in glycosyltransferase family 106 |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2020-07-01 |
description |
Rhamnogalacturonan I (RG-I) comprises approximately one quarter of the pectin molecules in land plants, and the backbone of RG-I consists of a repeating sequence of [2)-α-L-Rha(1-4)-α-D-GalUA(1-] disaccharide. Four Arabidopsis thaliana genes encoding RG-I rhamnosyltransferases (AtRRT1 to AtRRT4), which synthesize the disaccharide repeats, have been identified in the glycosyltransferase family (GT106). However, the functional role of RG-I in plant cell walls and the evolutional history of RRTs remains to be clarified. Here, we characterized the sole ortholog of AtRRT1–AtRRT4 in liverwort, Marchantia polymorpha, namely, MpRRT1. MpRRT1 had RRT activity and genetically complemented the AtRRT1-deficient mutant phenotype in A. thaliana. However, the MpRRT1-deficient M. polymorpha mutants showed no prominent morphological changes and only an approximate 20% reduction in rhamnose content in the cell wall fraction compared to that in wild-type plants, suggesting the existence of other RRT gene(s) in the M. polymorpha genome. As expected, we detected RRT activities in other GT106 family proteins such as those encoded by MpRRT3 in M. polymorpha and FRB1/AtRRT8 in A. thaliana, the deficient mutant of which affects cell adhesion. Our results show that RRT genes are more redundant and diverse in GT106 than previously thought. |
topic |
glycosyltransferase GT106 Marchantia polymorpha pectin rhamnogalacturonan I rhamnosyltransferase |
url |
https://www.frontiersin.org/article/10.3389/fpls.2020.00997/full |
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