Preparative Purification of Recombinant Proteins: Current Status and Future Trends
Advances in fermentation technologies have resulted in the production of increased yields of proteins of economic, biopharmaceutical, and medicinal importance. Consequently, there is an absolute requirement for the development of rapid, cost-effective methodologies which facilitate the purification...
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2013-01-01
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Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2013/312709 |
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doaj-bbc0e18bc5cf4fe99d8ba06a26c14af02020-11-24T23:01:55ZengHindawi LimitedBioMed Research International2314-61332314-61412013-01-01201310.1155/2013/312709312709Preparative Purification of Recombinant Proteins: Current Status and Future TrendsMayank Saraswat0Luca Musante1Alessandra Ravidá2Brian Shortt3Barry Byrne4Harry Holthofer5Centre for Bioanalytical Sciences (CBAS), Dublin City University (DCU), Dublin 9, IrelandCentre for Bioanalytical Sciences (CBAS), Dublin City University (DCU), Dublin 9, IrelandCentre for Bioanalytical Sciences (CBAS), Dublin City University (DCU), Dublin 9, IrelandCentre for Bioanalytical Sciences (CBAS), Dublin City University (DCU), Dublin 9, IrelandCentre for Bioanalytical Sciences (CBAS), Dublin City University (DCU), Dublin 9, IrelandCentre for Bioanalytical Sciences (CBAS), Dublin City University (DCU), Dublin 9, IrelandAdvances in fermentation technologies have resulted in the production of increased yields of proteins of economic, biopharmaceutical, and medicinal importance. Consequently, there is an absolute requirement for the development of rapid, cost-effective methodologies which facilitate the purification of such products in the absence of contaminants, such as superfluous proteins and endotoxins. Here, we provide a comprehensive overview of a selection of key purification methodologies currently being applied in both academic and industrial settings and discuss how innovative and effective protocols such as aqueous two-phase partitioning, membrane chromatography, and high-performance tangential flow filtration may be applied independently of or in conjunction with more traditional protocols for downstream processing applications.http://dx.doi.org/10.1155/2013/312709 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mayank Saraswat Luca Musante Alessandra Ravidá Brian Shortt Barry Byrne Harry Holthofer |
spellingShingle |
Mayank Saraswat Luca Musante Alessandra Ravidá Brian Shortt Barry Byrne Harry Holthofer Preparative Purification of Recombinant Proteins: Current Status and Future Trends BioMed Research International |
author_facet |
Mayank Saraswat Luca Musante Alessandra Ravidá Brian Shortt Barry Byrne Harry Holthofer |
author_sort |
Mayank Saraswat |
title |
Preparative Purification of Recombinant Proteins: Current Status and Future Trends |
title_short |
Preparative Purification of Recombinant Proteins: Current Status and Future Trends |
title_full |
Preparative Purification of Recombinant Proteins: Current Status and Future Trends |
title_fullStr |
Preparative Purification of Recombinant Proteins: Current Status and Future Trends |
title_full_unstemmed |
Preparative Purification of Recombinant Proteins: Current Status and Future Trends |
title_sort |
preparative purification of recombinant proteins: current status and future trends |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2013-01-01 |
description |
Advances in fermentation technologies have resulted in the production of increased yields of proteins of economic, biopharmaceutical, and medicinal importance. Consequently, there is an absolute requirement for the development of rapid, cost-effective methodologies which facilitate the purification of such products in the absence of contaminants, such as superfluous proteins and endotoxins. Here, we provide a comprehensive overview of a selection of key purification methodologies currently being applied in both academic and industrial settings and discuss how innovative and effective protocols such as aqueous two-phase partitioning, membrane chromatography, and high-performance tangential flow filtration may be applied independently of or in conjunction with more traditional protocols for downstream processing applications. |
url |
http://dx.doi.org/10.1155/2013/312709 |
work_keys_str_mv |
AT mayanksaraswat preparativepurificationofrecombinantproteinscurrentstatusandfuturetrends AT lucamusante preparativepurificationofrecombinantproteinscurrentstatusandfuturetrends AT alessandraravida preparativepurificationofrecombinantproteinscurrentstatusandfuturetrends AT brianshortt preparativepurificationofrecombinantproteinscurrentstatusandfuturetrends AT barrybyrne preparativepurificationofrecombinantproteinscurrentstatusandfuturetrends AT harryholthofer preparativepurificationofrecombinantproteinscurrentstatusandfuturetrends |
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1725638402889482240 |