Epidemiological survey of Neorickettsia risticii in equids from the State of Rio de Janeiro, Brazil
ABSTRACT: Equine neorickettsiosis (EN), also known as Potomac Horse Fever, is a non-contagious disease caused by the bacterium Neorickettsia risticii of the Anaplasmataceae family. The objectives of this study were to detect the presence of anti-N. risticii antibodies by the indirect immunofluoresce...
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doaj-bbcd731f4e8342b299a9659cda292fea2020-11-25T00:14:21ZengColégio Brasileiro de Patologia Animal (CBPA)Pesquisa Veterinária Brasileira1678-5150361093994610.1590/s0100-736x2016001000004S0100-736X2016001000939Epidemiological survey of Neorickettsia risticii in equids from the State of Rio de Janeiro, BrazilErica C.R. RoierRenata L. CostaMarcus S. PiresJoice A.R. VilelaTiago M. dos SantosHuarrisson A. SantosCristiane D. BaldaniCarlos L. MassardABSTRACT: Equine neorickettsiosis (EN), also known as Potomac Horse Fever, is a non-contagious disease caused by the bacterium Neorickettsia risticii of the Anaplasmataceae family. The objectives of this study were to detect the presence of anti-N. risticii antibodies by the indirect immunofluorescence assay (IFA) and of its DNA by qPCR in equids at high and low altitude regions in the State of Rio de Janeiro, Brazil, and to identify factors associated with seropositive equids by multiple logistic regression analysis. The frequency of anti-N. risticii antibodies was 16.05% (n=113/704). The animal age and breeding region were the factors that influenced the seropositivity rate for N. risticii in the equids (p<0.05). Equids from the lowland region had higher seropositivity (p<0.05; OR=5.87) compared to those of the mountain region. The presence of snails on the farm was a factor associated with this result (p<0.05; OR=2.88). In the lowland region, age of the animal and site of breeding were protective factors for the detection of antibodies anti-N. risticii in equids, with lower frequency of seropositivity in younger animals (p<0.05; OR=0.06) and in animals raised in dry areas (p<0.05; OR=0.22). The presence of the target DNA of N. risticii by qPCR was not observed in any of the samples tested. The existence of seropositive equids for N. risticii demonstrates a possible circulation of this agent in the studied area, and that the age related characteristics and equids breeding region are important factors regarding seropositivity in the State of Rio de Janeiro.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016001000939&lng=en&tlng=enNeorickettsia risticiiequídeosRio de JaneiroErliquiose Monocítica Equinadiagnóstico sorológicoepidemiologia |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Erica C.R. Roier Renata L. Costa Marcus S. Pires Joice A.R. Vilela Tiago M. dos Santos Huarrisson A. Santos Cristiane D. Baldani Carlos L. Massard |
spellingShingle |
Erica C.R. Roier Renata L. Costa Marcus S. Pires Joice A.R. Vilela Tiago M. dos Santos Huarrisson A. Santos Cristiane D. Baldani Carlos L. Massard Epidemiological survey of Neorickettsia risticii in equids from the State of Rio de Janeiro, Brazil Pesquisa Veterinária Brasileira Neorickettsia risticii equídeos Rio de Janeiro Erliquiose Monocítica Equina diagnóstico sorológico epidemiologia |
author_facet |
Erica C.R. Roier Renata L. Costa Marcus S. Pires Joice A.R. Vilela Tiago M. dos Santos Huarrisson A. Santos Cristiane D. Baldani Carlos L. Massard |
author_sort |
Erica C.R. Roier |
title |
Epidemiological survey of Neorickettsia risticii in equids from the State of Rio de Janeiro, Brazil |
title_short |
Epidemiological survey of Neorickettsia risticii in equids from the State of Rio de Janeiro, Brazil |
title_full |
Epidemiological survey of Neorickettsia risticii in equids from the State of Rio de Janeiro, Brazil |
title_fullStr |
Epidemiological survey of Neorickettsia risticii in equids from the State of Rio de Janeiro, Brazil |
title_full_unstemmed |
Epidemiological survey of Neorickettsia risticii in equids from the State of Rio de Janeiro, Brazil |
title_sort |
epidemiological survey of neorickettsia risticii in equids from the state of rio de janeiro, brazil |
publisher |
Colégio Brasileiro de Patologia Animal (CBPA) |
series |
Pesquisa Veterinária Brasileira |
issn |
1678-5150 |
description |
ABSTRACT: Equine neorickettsiosis (EN), also known as Potomac Horse Fever, is a non-contagious disease caused by the bacterium Neorickettsia risticii of the Anaplasmataceae family. The objectives of this study were to detect the presence of anti-N. risticii antibodies by the indirect immunofluorescence assay (IFA) and of its DNA by qPCR in equids at high and low altitude regions in the State of Rio de Janeiro, Brazil, and to identify factors associated with seropositive equids by multiple logistic regression analysis. The frequency of anti-N. risticii antibodies was 16.05% (n=113/704). The animal age and breeding region were the factors that influenced the seropositivity rate for N. risticii in the equids (p<0.05). Equids from the lowland region had higher seropositivity (p<0.05; OR=5.87) compared to those of the mountain region. The presence of snails on the farm was a factor associated with this result (p<0.05; OR=2.88). In the lowland region, age of the animal and site of breeding were protective factors for the detection of antibodies anti-N. risticii in equids, with lower frequency of seropositivity in younger animals (p<0.05; OR=0.06) and in animals raised in dry areas (p<0.05; OR=0.22). The presence of the target DNA of N. risticii by qPCR was not observed in any of the samples tested. The existence of seropositive equids for N. risticii demonstrates a possible circulation of this agent in the studied area, and that the age related characteristics and equids breeding region are important factors regarding seropositivity in the State of Rio de Janeiro. |
topic |
Neorickettsia risticii equídeos Rio de Janeiro Erliquiose Monocítica Equina diagnóstico sorológico epidemiologia |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016001000939&lng=en&tlng=en |
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