Cycloartane and Oleanane Glycosides from the Tubers of Eranthis cilicica
Phytochemical analysis of the tubers of Eranthis cilicica was performed as part of our continuous study on the plants of the family Ranunculaceae, which resulted in the isolation of eleven new cycloartane glycosides (1–11) and one new oleanane glycoside (13), together with one known oleana...
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doaj-bbd2770a835d4774aa2ad9b500ba10022020-11-25T00:17:37ZengMDPI AGMolecules1420-30492018-12-012416910.3390/molecules24010069molecules24010069Cycloartane and Oleanane Glycosides from the Tubers of Eranthis cilicicaKazuki Watanabe0Yoshihiro Mimaki1Haruhiko Fukaya2Yukiko Matsuo3Faculty of Pharmacy, Takasaki University of Health and Welfare, 37-1, Nakaorui, Takasaki, Gunma 370-0033, JapanDepartment of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Phramacy and Life Sciences, 1432-1, Horinouchi, Hachioji, Tokyo 192-0392, JapanDepartment of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Phramacy and Life Sciences, 1432-1, Horinouchi, Hachioji, Tokyo 192-0392, JapanDepartment of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Phramacy and Life Sciences, 1432-1, Horinouchi, Hachioji, Tokyo 192-0392, JapanPhytochemical analysis of the tubers of Eranthis cilicica was performed as part of our continuous study on the plants of the family Ranunculaceae, which resulted in the isolation of eleven new cycloartane glycosides (1–11) and one new oleanane glycoside (13), together with one known oleanane glycoside (12). The structures of the new compounds were determined by extensive spectroscopic analysis, including two-dimensional (2D) NMR, and enzymatic hydrolysis followed by either X-ray crystallographic or chromatographic analysis. The aglycone (1a) of 2 and its C-23 epimer (8a), and the oleanane glycosides (12 and 13) showed cytotoxic activity against HL-60 leukemia cells with IC50 values ranging from 10.6 μM to 101.6 μM. HL-60 cells were much more sensitive to 8a (IC50 14.8 μM) than 1a (IC50 101.1 μM), indicating that the C-23 configuration is associated with the cytotoxicity of these cycloartane derivatives. Compound 12 was revealed so as to partially induce apoptotic cell death in HL-60 cells, as was evident from morphology of HL-60 cells treated with 12.http://www.mdpi.com/1420-3049/24/1/69Eranthis cilicicaRanunculaceaecycloartane glycosideoleanane glycosidespectroscopic analysisX-ray crystallographic analysisapoptosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kazuki Watanabe Yoshihiro Mimaki Haruhiko Fukaya Yukiko Matsuo |
spellingShingle |
Kazuki Watanabe Yoshihiro Mimaki Haruhiko Fukaya Yukiko Matsuo Cycloartane and Oleanane Glycosides from the Tubers of Eranthis cilicica Molecules Eranthis cilicica Ranunculaceae cycloartane glycoside oleanane glycoside spectroscopic analysis X-ray crystallographic analysis apoptosis |
author_facet |
Kazuki Watanabe Yoshihiro Mimaki Haruhiko Fukaya Yukiko Matsuo |
author_sort |
Kazuki Watanabe |
title |
Cycloartane and Oleanane Glycosides from the Tubers of Eranthis cilicica |
title_short |
Cycloartane and Oleanane Glycosides from the Tubers of Eranthis cilicica |
title_full |
Cycloartane and Oleanane Glycosides from the Tubers of Eranthis cilicica |
title_fullStr |
Cycloartane and Oleanane Glycosides from the Tubers of Eranthis cilicica |
title_full_unstemmed |
Cycloartane and Oleanane Glycosides from the Tubers of Eranthis cilicica |
title_sort |
cycloartane and oleanane glycosides from the tubers of eranthis cilicica |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2018-12-01 |
description |
Phytochemical analysis of the tubers of Eranthis cilicica was performed as part of our continuous study on the plants of the family Ranunculaceae, which resulted in the isolation of eleven new cycloartane glycosides (1–11) and one new oleanane glycoside (13), together with one known oleanane glycoside (12). The structures of the new compounds were determined by extensive spectroscopic analysis, including two-dimensional (2D) NMR, and enzymatic hydrolysis followed by either X-ray crystallographic or chromatographic analysis. The aglycone (1a) of 2 and its C-23 epimer (8a), and the oleanane glycosides (12 and 13) showed cytotoxic activity against HL-60 leukemia cells with IC50 values ranging from 10.6 μM to 101.6 μM. HL-60 cells were much more sensitive to 8a (IC50 14.8 μM) than 1a (IC50 101.1 μM), indicating that the C-23 configuration is associated with the cytotoxicity of these cycloartane derivatives. Compound 12 was revealed so as to partially induce apoptotic cell death in HL-60 cells, as was evident from morphology of HL-60 cells treated with 12. |
topic |
Eranthis cilicica Ranunculaceae cycloartane glycoside oleanane glycoside spectroscopic analysis X-ray crystallographic analysis apoptosis |
url |
http://www.mdpi.com/1420-3049/24/1/69 |
work_keys_str_mv |
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