Protein Oxidation Biomarkers and Myeloperoxidase Activation in Cerebrospinal Fluid in Childhood Bacterial Meningitis
The immunological response in bacterial meningitis (BM) causes the formation of reactive oxygen and nitrogen species (ROS, RNS) and activates myeloperoxidase (MPO), an inflammatory enzyme. Thus, structural oxidative and nitrosative damage to proteins and DNA occurs. We aimed to asses these events in...
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2019-10-01
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doaj-bbd881064d5240a29b7092d7a8e2603d2020-11-25T01:18:38ZengMDPI AGAntioxidants2076-39212019-10-0181044110.3390/antiox8100441antiox8100441Protein Oxidation Biomarkers and Myeloperoxidase Activation in Cerebrospinal Fluid in Childhood Bacterial MeningitisEmilie Rugemalira0Irmeli Roine1Julia Kuligowski2Ángel Sánchez-Illana3José David Piñeiro-Ramos4Sture Andersson5Heikki Peltola6Manuel Leite Cruzeiro7Tuula Pelkonen8Máximo Vento9Children’s Hospital, Helsinki University Hospital, Stenbäckinkatu 9, 00029 Helsinki, FinlandFaculty of Medicine, University Diego Portales, Manuel Rodrigues Sur 333, 8370109 Santiago Region Metropolitana, ChileHealth Research Institute La Fe, Avenida Fernando Abril Martorell 106, 46026 Valencia, SpainHealth Research Institute La Fe, Avenida Fernando Abril Martorell 106, 46026 Valencia, SpainHealth Research Institute La Fe, Avenida Fernando Abril Martorell 106, 46026 Valencia, SpainChildren’s Hospital, Helsinki University Hospital, Stenbäckinkatu 9, 00029 Helsinki, FinlandChildren’s Hospital, Helsinki University Hospital, Stenbäckinkatu 9, 00029 Helsinki, FinlandHospital Pediátrico David Bernardino, Rua Amilcar Cabral, Luanda, AngolaChildren’s Hospital, Helsinki University Hospital, Stenbäckinkatu 9, 00029 Helsinki, FinlandHealth Research Institute La Fe, Avenida Fernando Abril Martorell 106, 46026 Valencia, SpainThe immunological response in bacterial meningitis (BM) causes the formation of reactive oxygen and nitrogen species (ROS, RNS) and activates myeloperoxidase (MPO), an inflammatory enzyme. Thus, structural oxidative and nitrosative damage to proteins and DNA occurs. We aimed to asses these events in the cerebrospinal fluid (CSF) of pediatric BM patients. Phenylalanine (Phe), para-tyrosine (p-Tyr), nucleoside 2′-deoxiguanosine (2dG), and biomarkers of ROS/RNS-induced protein and DNA oxidation: ortho-tyrosine (o-Tyr), 3-chlorotyrosine (3Cl-Tyr), 3-nitrotyrosine (3NO₂-Tyr) and 8-oxo-2′-deoxyguanosine (8OHdG), concentrations were measured by liquid chromatography coupled to tandem mass spectrometry in the initial CSF of 79 children with BM and 10 without BM. All biomarkers, normalized with their corresponding precursors, showed higher median concentrations (<i>p</i> < 0.0001) in BM compared with controls, except 8OHdG/2dG. The ratios o-Tyr/Phe, 3Cl-Tyr/p-Tyr and 3NO₂-Tyr/p-Tyr were 570, 20 and 4.5 times as high, respectively. A significantly higher 3Cl-Tyr/p-Tyr ratio was found in BM caused by <i>Streptococcus pneumoniae</i>, than by <i>Haemophilus influenzae</i> type b, or <i>Neisseria meningitidis</i> (<i>p</i> = 0.002 for both). In conclusion, biomarkers indicating oxidative damage to proteins distinguished BM patients from non-BM, most clearly the o-Tyr/Phe ratio. The high 3Cl-Tyr/p-Tyr ratio in pneumococcal meningitis suggests robust inflammation because 3Cl-Tyr is a marker of MPO activation and, indirectly, of inflammation.https://www.mdpi.com/2076-3921/8/10/441oxidative stressprotein damagemyeloperoxidasebacterial meningitisdeveloping countries |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Emilie Rugemalira Irmeli Roine Julia Kuligowski Ángel Sánchez-Illana José David Piñeiro-Ramos Sture Andersson Heikki Peltola Manuel Leite Cruzeiro Tuula Pelkonen Máximo Vento |
spellingShingle |
Emilie Rugemalira Irmeli Roine Julia Kuligowski Ángel Sánchez-Illana José David Piñeiro-Ramos Sture Andersson Heikki Peltola Manuel Leite Cruzeiro Tuula Pelkonen Máximo Vento Protein Oxidation Biomarkers and Myeloperoxidase Activation in Cerebrospinal Fluid in Childhood Bacterial Meningitis Antioxidants oxidative stress protein damage myeloperoxidase bacterial meningitis developing countries |
author_facet |
Emilie Rugemalira Irmeli Roine Julia Kuligowski Ángel Sánchez-Illana José David Piñeiro-Ramos Sture Andersson Heikki Peltola Manuel Leite Cruzeiro Tuula Pelkonen Máximo Vento |
author_sort |
Emilie Rugemalira |
title |
Protein Oxidation Biomarkers and Myeloperoxidase Activation in Cerebrospinal Fluid in Childhood Bacterial Meningitis |
title_short |
Protein Oxidation Biomarkers and Myeloperoxidase Activation in Cerebrospinal Fluid in Childhood Bacterial Meningitis |
title_full |
Protein Oxidation Biomarkers and Myeloperoxidase Activation in Cerebrospinal Fluid in Childhood Bacterial Meningitis |
title_fullStr |
Protein Oxidation Biomarkers and Myeloperoxidase Activation in Cerebrospinal Fluid in Childhood Bacterial Meningitis |
title_full_unstemmed |
Protein Oxidation Biomarkers and Myeloperoxidase Activation in Cerebrospinal Fluid in Childhood Bacterial Meningitis |
title_sort |
protein oxidation biomarkers and myeloperoxidase activation in cerebrospinal fluid in childhood bacterial meningitis |
publisher |
MDPI AG |
series |
Antioxidants |
issn |
2076-3921 |
publishDate |
2019-10-01 |
description |
The immunological response in bacterial meningitis (BM) causes the formation of reactive oxygen and nitrogen species (ROS, RNS) and activates myeloperoxidase (MPO), an inflammatory enzyme. Thus, structural oxidative and nitrosative damage to proteins and DNA occurs. We aimed to asses these events in the cerebrospinal fluid (CSF) of pediatric BM patients. Phenylalanine (Phe), para-tyrosine (p-Tyr), nucleoside 2′-deoxiguanosine (2dG), and biomarkers of ROS/RNS-induced protein and DNA oxidation: ortho-tyrosine (o-Tyr), 3-chlorotyrosine (3Cl-Tyr), 3-nitrotyrosine (3NO₂-Tyr) and 8-oxo-2′-deoxyguanosine (8OHdG), concentrations were measured by liquid chromatography coupled to tandem mass spectrometry in the initial CSF of 79 children with BM and 10 without BM. All biomarkers, normalized with their corresponding precursors, showed higher median concentrations (<i>p</i> < 0.0001) in BM compared with controls, except 8OHdG/2dG. The ratios o-Tyr/Phe, 3Cl-Tyr/p-Tyr and 3NO₂-Tyr/p-Tyr were 570, 20 and 4.5 times as high, respectively. A significantly higher 3Cl-Tyr/p-Tyr ratio was found in BM caused by <i>Streptococcus pneumoniae</i>, than by <i>Haemophilus influenzae</i> type b, or <i>Neisseria meningitidis</i> (<i>p</i> = 0.002 for both). In conclusion, biomarkers indicating oxidative damage to proteins distinguished BM patients from non-BM, most clearly the o-Tyr/Phe ratio. The high 3Cl-Tyr/p-Tyr ratio in pneumococcal meningitis suggests robust inflammation because 3Cl-Tyr is a marker of MPO activation and, indirectly, of inflammation. |
topic |
oxidative stress protein damage myeloperoxidase bacterial meningitis developing countries |
url |
https://www.mdpi.com/2076-3921/8/10/441 |
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