Cadmium exposure promotes activation of cerebrum and cerebellum ferroptosis and necrosis in swine
Cadmium(Cd) is a toxic and carcinogenic heavy metal pollutant leading to serious damage in various organs. Ferroptosis and necrosis as inflammation-related cell death are involved in several diseases of nervous system. In the present study, 10 weaning piglets with similar weight for 6 weeks were ran...
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2021-11-01
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doaj-bbe70d4971a74efcbecf41699e0ef4c42021-09-05T04:38:20ZengElsevierEcotoxicology and Environmental Safety0147-65132021-11-01224112650Cadmium exposure promotes activation of cerebrum and cerebellum ferroptosis and necrosis in swinePeixian Luan0Yue Sun1Yue Zhu2Senqiu Qiao3Guo Hu4Qi Liu5Ziwei Zhang6Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 0150070, PR China; Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150070, PR ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR ChinaHeilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 0150070, PR China; Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150070, PR China; Corresponding author at: Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, PR China.College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China; Corresponding authors.College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China; Corresponding authors.Cadmium(Cd) is a toxic and carcinogenic heavy metal pollutant leading to serious damage in various organs. Ferroptosis and necrosis as inflammation-related cell death are involved in several diseases of nervous system. In the present study, 10 weaning piglets with similar weight for 6 weeks were randomly divided into two groups. The daily grain containing 0 mg and 20 mg/kg of Cd chloride was fed in 20–26 ℃ environment, animals were sacrificed to collect cerebrum and cerebellum tissues after 40 days. Morphology and ultrastructure results were observed using HE and TEM. Moreover, molecular biological technologies western blot and qRT-PCR were used to detect the expression abundance of genes. Cerebrum and cerebellum injury was observed in Cd-exposed group, antioxidant capacity decreased significantly and oxidative stress increased; immunofluorescence, real-time quantification, and western blot results showed decreased necrosis genes and increased ferroptosis pathway genes abundance in cerebrum, whereas the results were reversed in cerebellum. These results indicated that Cd exposure can activated necrosis and ferroptosis pathways by increased oxidative stress, further resulting in cerebrum and cerebellum damage in pigs. These findings may provide a theoretical basis for early monitoring of Cd exposure in environment.http://www.sciencedirect.com/science/article/pii/S0147651321007624FerroptosisNecroptosisCerebrumCerebellumCadmium |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Peixian Luan Yue Sun Yue Zhu Senqiu Qiao Guo Hu Qi Liu Ziwei Zhang |
spellingShingle |
Peixian Luan Yue Sun Yue Zhu Senqiu Qiao Guo Hu Qi Liu Ziwei Zhang Cadmium exposure promotes activation of cerebrum and cerebellum ferroptosis and necrosis in swine Ecotoxicology and Environmental Safety Ferroptosis Necroptosis Cerebrum Cerebellum Cadmium |
author_facet |
Peixian Luan Yue Sun Yue Zhu Senqiu Qiao Guo Hu Qi Liu Ziwei Zhang |
author_sort |
Peixian Luan |
title |
Cadmium exposure promotes activation of cerebrum and cerebellum ferroptosis and necrosis in swine |
title_short |
Cadmium exposure promotes activation of cerebrum and cerebellum ferroptosis and necrosis in swine |
title_full |
Cadmium exposure promotes activation of cerebrum and cerebellum ferroptosis and necrosis in swine |
title_fullStr |
Cadmium exposure promotes activation of cerebrum and cerebellum ferroptosis and necrosis in swine |
title_full_unstemmed |
Cadmium exposure promotes activation of cerebrum and cerebellum ferroptosis and necrosis in swine |
title_sort |
cadmium exposure promotes activation of cerebrum and cerebellum ferroptosis and necrosis in swine |
publisher |
Elsevier |
series |
Ecotoxicology and Environmental Safety |
issn |
0147-6513 |
publishDate |
2021-11-01 |
description |
Cadmium(Cd) is a toxic and carcinogenic heavy metal pollutant leading to serious damage in various organs. Ferroptosis and necrosis as inflammation-related cell death are involved in several diseases of nervous system. In the present study, 10 weaning piglets with similar weight for 6 weeks were randomly divided into two groups. The daily grain containing 0 mg and 20 mg/kg of Cd chloride was fed in 20–26 ℃ environment, animals were sacrificed to collect cerebrum and cerebellum tissues after 40 days. Morphology and ultrastructure results were observed using HE and TEM. Moreover, molecular biological technologies western blot and qRT-PCR were used to detect the expression abundance of genes. Cerebrum and cerebellum injury was observed in Cd-exposed group, antioxidant capacity decreased significantly and oxidative stress increased; immunofluorescence, real-time quantification, and western blot results showed decreased necrosis genes and increased ferroptosis pathway genes abundance in cerebrum, whereas the results were reversed in cerebellum. These results indicated that Cd exposure can activated necrosis and ferroptosis pathways by increased oxidative stress, further resulting in cerebrum and cerebellum damage in pigs. These findings may provide a theoretical basis for early monitoring of Cd exposure in environment. |
topic |
Ferroptosis Necroptosis Cerebrum Cerebellum Cadmium |
url |
http://www.sciencedirect.com/science/article/pii/S0147651321007624 |
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