Effects of Bacterial Translocation and Autophagy on Acute Lung Injury Induced by Severe Acute Pancreatitis

Aim. To reveal the role of bacterial translocation (BT) and autophagy in severe acute pancreatitis-induced acute lung injury (SAP-ALI). Methods. Rats were separated into a control (sham-operation) group (n=10) and a SAP group (n=30). Sodium taurocholate (5%) was retrogradely injected into the cholan...

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Main Authors: Hanlin Wang, Chang Li, Yingjian Jiang, Hongbo Li, Dianliang Zhang
Format: Article
Language:English
Published: Hindawi Limited 2020-01-01
Series:Gastroenterology Research and Practice
Online Access:http://dx.doi.org/10.1155/2020/8953453
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spelling doaj-bbf8d02144e346bc8c6dfe304813b8952020-11-25T00:35:11ZengHindawi LimitedGastroenterology Research and Practice1687-61211687-630X2020-01-01202010.1155/2020/89534538953453Effects of Bacterial Translocation and Autophagy on Acute Lung Injury Induced by Severe Acute PancreatitisHanlin Wang0Chang Li1Yingjian Jiang2Hongbo Li3Dianliang Zhang4Center of Colon and Rectum, Qingdao Municipal Hospital, Qingdao University, No. 1 Jiaozhou Road, Qingdao, 266011 Shandong Province, ChinaCenter of Colon and Rectum, Qingdao Municipal Hospital, Qingdao University, No. 1 Jiaozhou Road, Qingdao, 266011 Shandong Province, ChinaCenter of Colon and Rectum, Qingdao Municipal Hospital, Qingdao University, No. 1 Jiaozhou Road, Qingdao, 266011 Shandong Province, ChinaCenter of Colon and Rectum, Qingdao Municipal Hospital, Qingdao University, No. 1 Jiaozhou Road, Qingdao, 266011 Shandong Province, ChinaCenter of Colon and Rectum, Qingdao Municipal Hospital, Qingdao University, No. 1 Jiaozhou Road, Qingdao, 266011 Shandong Province, ChinaAim. To reveal the role of bacterial translocation (BT) and autophagy in severe acute pancreatitis-induced acute lung injury (SAP-ALI). Methods. Rats were separated into a control (sham-operation) group (n=10) and a SAP group (n=30). Sodium taurocholate (5%) was retrogradely injected into the cholangiopancreatic duct to induce SAP-ALI in rats. Then, 16S rDNA sequencing was used to detect bacterial translocation (BT). Hematoxylin eosin staining (HE) was used to detect morphological changes to the pancreas, intestine, and lung. And lung tissue wet/dry weight ratio (W/D ratio) was used to assess the extent of pulmonary edema. The expressions of LC3II and Beclin1 proteins were analyzed by western blot and immunofluorescence. Glutathione peroxidase (GPx), malondialdehyde (MDA), and superoxide dismutase (SOD) were used to assess oxidative stress in lung tissue. Results. Levels of TNF-α, IL-6, lipase, and amylase in the SAP group were significantly higher than those in the control group (P<0.01). Histopathological score and W/D ratio of the lung in the SAP-BT(+) group were significantly higher than that in the SAP-BT(-) group (P<0.01). LC3II expression was higher in the SAP-BT(-) group than that in the SAP-BT(+) group (P<0.01). The results were consistent with those of LC3II immunofluorescence assay. The expression of Beclin1 was similar to that of LC3II (P<0.01). MDA content in the SAP-BT(+) group was significantly higher than that in the SAP-BT(-) group (P<0.01), whereas SOD and GPX activities were opposite (P<0.01). Conclusions. BT can aggravate SAP-ALI with the increasing oxidative stress level, which may be related to the decrease of autophagy level.http://dx.doi.org/10.1155/2020/8953453
collection DOAJ
language English
format Article
sources DOAJ
author Hanlin Wang
Chang Li
Yingjian Jiang
Hongbo Li
Dianliang Zhang
spellingShingle Hanlin Wang
Chang Li
Yingjian Jiang
Hongbo Li
Dianliang Zhang
Effects of Bacterial Translocation and Autophagy on Acute Lung Injury Induced by Severe Acute Pancreatitis
Gastroenterology Research and Practice
author_facet Hanlin Wang
Chang Li
Yingjian Jiang
Hongbo Li
Dianliang Zhang
author_sort Hanlin Wang
title Effects of Bacterial Translocation and Autophagy on Acute Lung Injury Induced by Severe Acute Pancreatitis
title_short Effects of Bacterial Translocation and Autophagy on Acute Lung Injury Induced by Severe Acute Pancreatitis
title_full Effects of Bacterial Translocation and Autophagy on Acute Lung Injury Induced by Severe Acute Pancreatitis
title_fullStr Effects of Bacterial Translocation and Autophagy on Acute Lung Injury Induced by Severe Acute Pancreatitis
title_full_unstemmed Effects of Bacterial Translocation and Autophagy on Acute Lung Injury Induced by Severe Acute Pancreatitis
title_sort effects of bacterial translocation and autophagy on acute lung injury induced by severe acute pancreatitis
publisher Hindawi Limited
series Gastroenterology Research and Practice
issn 1687-6121
1687-630X
publishDate 2020-01-01
description Aim. To reveal the role of bacterial translocation (BT) and autophagy in severe acute pancreatitis-induced acute lung injury (SAP-ALI). Methods. Rats were separated into a control (sham-operation) group (n=10) and a SAP group (n=30). Sodium taurocholate (5%) was retrogradely injected into the cholangiopancreatic duct to induce SAP-ALI in rats. Then, 16S rDNA sequencing was used to detect bacterial translocation (BT). Hematoxylin eosin staining (HE) was used to detect morphological changes to the pancreas, intestine, and lung. And lung tissue wet/dry weight ratio (W/D ratio) was used to assess the extent of pulmonary edema. The expressions of LC3II and Beclin1 proteins were analyzed by western blot and immunofluorescence. Glutathione peroxidase (GPx), malondialdehyde (MDA), and superoxide dismutase (SOD) were used to assess oxidative stress in lung tissue. Results. Levels of TNF-α, IL-6, lipase, and amylase in the SAP group were significantly higher than those in the control group (P<0.01). Histopathological score and W/D ratio of the lung in the SAP-BT(+) group were significantly higher than that in the SAP-BT(-) group (P<0.01). LC3II expression was higher in the SAP-BT(-) group than that in the SAP-BT(+) group (P<0.01). The results were consistent with those of LC3II immunofluorescence assay. The expression of Beclin1 was similar to that of LC3II (P<0.01). MDA content in the SAP-BT(+) group was significantly higher than that in the SAP-BT(-) group (P<0.01), whereas SOD and GPX activities were opposite (P<0.01). Conclusions. BT can aggravate SAP-ALI with the increasing oxidative stress level, which may be related to the decrease of autophagy level.
url http://dx.doi.org/10.1155/2020/8953453
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