Comparative study of hematopoietic differentiation between human embryonic stem cell lines.
Directed differentiation of human embryonic stem cells (hESCs) into any desired cell type has been hailed as a therapeutic promise to cure many human diseases. However, substantial roadblocks still exist for in vitro differentiation of hESCs into distinct cell types, including T lymphocytes. Here we...
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2011-01-01
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doaj-bc2b318e34d84e0d8b623bda43341e432020-11-25T01:47:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0165e1985410.1371/journal.pone.0019854Comparative study of hematopoietic differentiation between human embryonic stem cell lines.Heather MelicharOu LiJenny RossHilary HaberDragana CadoHector NollaEllen A RobeyAstar WinotoDirected differentiation of human embryonic stem cells (hESCs) into any desired cell type has been hailed as a therapeutic promise to cure many human diseases. However, substantial roadblocks still exist for in vitro differentiation of hESCs into distinct cell types, including T lymphocytes. Here we examined the hematopoietic differentiation potential of six different hESC lines. We compare their ability to develop into CD34(+) or CD34(+)CD45(+) hematopoietic precursor populations under several differentiation conditions. Comparison of lymphoid potential of hESC derived- and fetal tissue derived-hematopoietic precursors was also made. We found diverse hematopoietic potential between hESC lines depending on the culture or passage conditions. In contrast to fetal-derived hematopoietic precursors, none of the CD34(+) precursors differentiated from hESCs were able to develop further into T cells. These data underscore the difficulties in the current strategy of hESC forward differentiation and highlight distinct differences between CD34(+) hematopoietic precursors generated in vitro versus in vivo.http://europepmc.org/articles/PMC3095633?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Heather Melichar Ou Li Jenny Ross Hilary Haber Dragana Cado Hector Nolla Ellen A Robey Astar Winoto |
spellingShingle |
Heather Melichar Ou Li Jenny Ross Hilary Haber Dragana Cado Hector Nolla Ellen A Robey Astar Winoto Comparative study of hematopoietic differentiation between human embryonic stem cell lines. PLoS ONE |
author_facet |
Heather Melichar Ou Li Jenny Ross Hilary Haber Dragana Cado Hector Nolla Ellen A Robey Astar Winoto |
author_sort |
Heather Melichar |
title |
Comparative study of hematopoietic differentiation between human embryonic stem cell lines. |
title_short |
Comparative study of hematopoietic differentiation between human embryonic stem cell lines. |
title_full |
Comparative study of hematopoietic differentiation between human embryonic stem cell lines. |
title_fullStr |
Comparative study of hematopoietic differentiation between human embryonic stem cell lines. |
title_full_unstemmed |
Comparative study of hematopoietic differentiation between human embryonic stem cell lines. |
title_sort |
comparative study of hematopoietic differentiation between human embryonic stem cell lines. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-01-01 |
description |
Directed differentiation of human embryonic stem cells (hESCs) into any desired cell type has been hailed as a therapeutic promise to cure many human diseases. However, substantial roadblocks still exist for in vitro differentiation of hESCs into distinct cell types, including T lymphocytes. Here we examined the hematopoietic differentiation potential of six different hESC lines. We compare their ability to develop into CD34(+) or CD34(+)CD45(+) hematopoietic precursor populations under several differentiation conditions. Comparison of lymphoid potential of hESC derived- and fetal tissue derived-hematopoietic precursors was also made. We found diverse hematopoietic potential between hESC lines depending on the culture or passage conditions. In contrast to fetal-derived hematopoietic precursors, none of the CD34(+) precursors differentiated from hESCs were able to develop further into T cells. These data underscore the difficulties in the current strategy of hESC forward differentiation and highlight distinct differences between CD34(+) hematopoietic precursors generated in vitro versus in vivo. |
url |
http://europepmc.org/articles/PMC3095633?pdf=render |
work_keys_str_mv |
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