Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São Paulo
Brazilian spotted fever (BSF) cases have been increasing in the state of São Paulo but no genomic information about local rickettsia isolated from humans has been well documented. We recovered spotted-fever group rickettsiae from a sample of patient blood cultured in Vero cells using the shell vial...
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| Format: | Article |
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Instituto Oswaldo Cruz, Ministério da Saúde
2005-05-01
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| Series: | Memórias do Instituto Oswaldo Cruz. |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000300011 |
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doaj-bc30b9ac6f2c4b02874cde88fbda58f32020-11-24T22:26:27ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.0074-02761678-80602005-05-01100327727910.1590/S0074-02762005000300011Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São PauloElvira Maria Mendes NascimentoFlávia de Sousa GehrkeRosa Amélia MaldonadoSilvia ColomboLuiz Jacintho da SilvaTeresinha Tizu Sato SchumakerBrazilian spotted fever (BSF) cases have been increasing in the state of São Paulo but no genomic information about local rickettsia isolated from humans has been well documented. We recovered spotted-fever group rickettsiae from a sample of patient blood cultured in Vero cells using the shell vial technique. Rickettsial DNA fragments (gltA, ompA, and, ompB genes) were detected, and analysis of the ompB gene base sequences showed identity with the Rickettsia rickettsii ompB sequence available in the GenBank.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000300011Brazilian spotted feverRickettsia rickettsiiompB gene |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Elvira Maria Mendes Nascimento Flávia de Sousa Gehrke Rosa Amélia Maldonado Silvia Colombo Luiz Jacintho da Silva Teresinha Tizu Sato Schumaker |
| spellingShingle |
Elvira Maria Mendes Nascimento Flávia de Sousa Gehrke Rosa Amélia Maldonado Silvia Colombo Luiz Jacintho da Silva Teresinha Tizu Sato Schumaker Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São Paulo Memórias do Instituto Oswaldo Cruz. Brazilian spotted fever Rickettsia rickettsii ompB gene |
| author_facet |
Elvira Maria Mendes Nascimento Flávia de Sousa Gehrke Rosa Amélia Maldonado Silvia Colombo Luiz Jacintho da Silva Teresinha Tizu Sato Schumaker |
| author_sort |
Elvira Maria Mendes Nascimento |
| title |
Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São Paulo |
| title_short |
Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São Paulo |
| title_full |
Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São Paulo |
| title_fullStr |
Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São Paulo |
| title_full_unstemmed |
Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São Paulo |
| title_sort |
detection of brazilian spotted fever infection by polymerase chain reaction in a patient from the state of são paulo |
| publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
| series |
Memórias do Instituto Oswaldo Cruz. |
| issn |
0074-0276 1678-8060 |
| publishDate |
2005-05-01 |
| description |
Brazilian spotted fever (BSF) cases have been increasing in the state of São Paulo but no genomic information about local rickettsia isolated from humans has been well documented. We recovered spotted-fever group rickettsiae from a sample of patient blood cultured in Vero cells using the shell vial technique. Rickettsial DNA fragments (gltA, ompA, and, ompB genes) were detected, and analysis of the ompB gene base sequences showed identity with the Rickettsia rickettsii ompB sequence available in the GenBank. |
| topic |
Brazilian spotted fever Rickettsia rickettsii ompB gene |
| url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000300011 |
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