<i>CaCML13</i> Acts Positively in Pepper Immunity Against <i>Ralstonia solanacearum</i> Infection Forming Feedback Loop with CabZIP63
Ca<sup>2+</sup>-signaling—which requires the presence of calcium sensors such as calmodulin (CaM) and calmodulin-like (CML) proteins—is crucial for the regulation of plant immunity against pathogen attack. However, the underlying mechanisms remain elusive, especially the roles of CMLs in...
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doaj-bc366e3f523f4010915eab2ce607e7502020-11-25T03:11:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-06-01214186418610.3390/ijms21114186<i>CaCML13</i> Acts Positively in Pepper Immunity Against <i>Ralstonia solanacearum</i> Infection Forming Feedback Loop with CabZIP63Lei Shen0Sheng Yang1Deyi Guan2Shuilin He3National Education Ministry Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, ChinaNational Education Ministry Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, ChinaNational Education Ministry Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, ChinaNational Education Ministry Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, ChinaCa<sup>2+</sup>-signaling—which requires the presence of calcium sensors such as calmodulin (CaM) and calmodulin-like (CML) proteins—is crucial for the regulation of plant immunity against pathogen attack. However, the underlying mechanisms remain elusive, especially the roles of CMLs involved in plant immunity remains largely uninvestigated. In the present study, <i>CaCML13</i>, a calmodulin-like protein of pepper that was originally found to be upregulated by <i>Ralstonia solanacearum </i>inoculation (RSI) in RNA-seq, was functionally characterized in immunity against RSI. CaCML13 was found to target the whole epidermal cell including plasma membrane, cytoplasm and nucleus. We also confirmed that<i> CaCML13</i> was upregulated by RSI in pepper roots by quantitative real-time PCR (qRT-PCR). The silencing of <i>CaCML13</i> significantly enhanced pepper plants’ susceptibility to RSI accompanied with downregulation of immunity-related <i>CaPR1</i>, <i>CaNPR1</i>, <i>CaDEF1</i> and <i>CabZIP63</i>. In contrast, <i>CaCML13</i> transient overexpression induced clear hypersensitivity-reaction (HR)-mimicked cell death and upregulation of the tested immunity-related genes. In addition, we also revealed that the G-box-containing <i>CaCML13</i> promoter was bound by CabZIP63 and <i>CaCML13</i> was positively regulated by CabZIP63 at transcriptional level. Our data collectively indicate that CaCML13 act as a positive regulator in pepper immunity against RSI forming a positive feedback loop with <i>CabZIP63</i>.https://www.mdpi.com/1422-0067/21/11/4186<i>Capsicum</i> <i>annuum</i>CaCML13CabZIP63immunity<i>Ralstonia solanacearum</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lei Shen Sheng Yang Deyi Guan Shuilin He |
spellingShingle |
Lei Shen Sheng Yang Deyi Guan Shuilin He <i>CaCML13</i> Acts Positively in Pepper Immunity Against <i>Ralstonia solanacearum</i> Infection Forming Feedback Loop with CabZIP63 International Journal of Molecular Sciences <i>Capsicum</i> <i>annuum</i> CaCML13 CabZIP63 immunity <i>Ralstonia solanacearum</i> |
author_facet |
Lei Shen Sheng Yang Deyi Guan Shuilin He |
author_sort |
Lei Shen |
title |
<i>CaCML13</i> Acts Positively in Pepper Immunity Against <i>Ralstonia solanacearum</i> Infection Forming Feedback Loop with CabZIP63 |
title_short |
<i>CaCML13</i> Acts Positively in Pepper Immunity Against <i>Ralstonia solanacearum</i> Infection Forming Feedback Loop with CabZIP63 |
title_full |
<i>CaCML13</i> Acts Positively in Pepper Immunity Against <i>Ralstonia solanacearum</i> Infection Forming Feedback Loop with CabZIP63 |
title_fullStr |
<i>CaCML13</i> Acts Positively in Pepper Immunity Against <i>Ralstonia solanacearum</i> Infection Forming Feedback Loop with CabZIP63 |
title_full_unstemmed |
<i>CaCML13</i> Acts Positively in Pepper Immunity Against <i>Ralstonia solanacearum</i> Infection Forming Feedback Loop with CabZIP63 |
title_sort |
<i>cacml13</i> acts positively in pepper immunity against <i>ralstonia solanacearum</i> infection forming feedback loop with cabzip63 |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-06-01 |
description |
Ca<sup>2+</sup>-signaling—which requires the presence of calcium sensors such as calmodulin (CaM) and calmodulin-like (CML) proteins—is crucial for the regulation of plant immunity against pathogen attack. However, the underlying mechanisms remain elusive, especially the roles of CMLs involved in plant immunity remains largely uninvestigated. In the present study, <i>CaCML13</i>, a calmodulin-like protein of pepper that was originally found to be upregulated by <i>Ralstonia solanacearum </i>inoculation (RSI) in RNA-seq, was functionally characterized in immunity against RSI. CaCML13 was found to target the whole epidermal cell including plasma membrane, cytoplasm and nucleus. We also confirmed that<i> CaCML13</i> was upregulated by RSI in pepper roots by quantitative real-time PCR (qRT-PCR). The silencing of <i>CaCML13</i> significantly enhanced pepper plants’ susceptibility to RSI accompanied with downregulation of immunity-related <i>CaPR1</i>, <i>CaNPR1</i>, <i>CaDEF1</i> and <i>CabZIP63</i>. In contrast, <i>CaCML13</i> transient overexpression induced clear hypersensitivity-reaction (HR)-mimicked cell death and upregulation of the tested immunity-related genes. In addition, we also revealed that the G-box-containing <i>CaCML13</i> promoter was bound by CabZIP63 and <i>CaCML13</i> was positively regulated by CabZIP63 at transcriptional level. Our data collectively indicate that CaCML13 act as a positive regulator in pepper immunity against RSI forming a positive feedback loop with <i>CabZIP63</i>. |
topic |
<i>Capsicum</i> <i>annuum</i> CaCML13 CabZIP63 immunity <i>Ralstonia solanacearum</i> |
url |
https://www.mdpi.com/1422-0067/21/11/4186 |
work_keys_str_mv |
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