Enrichment and Purification of the Bioactive Flavonoids from Flower of Abelmoschus manihot (L.) Medic Using Macroporous Resins
Flower of Abelmoschus manihot (FAM) is clinically effective to treat chronic kidney disease (CKD) with a relatively high dosage. To improve the efficacy and the compliance of patients, macroporous resins were adopted to enrich and purify flavonoids from FAM, which are thought to be the major renal p...
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doaj-bc3cb60332fb4ebdaa394e11e07518252020-11-24T23:04:25ZengMDPI AGMolecules1420-30492018-10-012310264910.3390/molecules23102649molecules23102649Enrichment and Purification of the Bioactive Flavonoids from Flower of Abelmoschus manihot (L.) Medic Using Macroporous ResinsZhenzhong Yang0Haitao Tang1Qing Shao2Anna Rita Bilia3Yi Wang4Xiaoping Zhao5Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, ChinaSZYY Group Pharmaceutical Limited, Taizhou 225500, ChinaPharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, ChinaDepartment of Chemistry, University of Florence, via Ugo Schiff 6, Sesto Fiorentino, 50019 Florence, ItalyPharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, ChinaSchool of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaFlower of Abelmoschus manihot (FAM) is clinically effective to treat chronic kidney disease (CKD) with a relatively high dosage. To improve the efficacy and the compliance of patients, macroporous resins were adopted to enrich and purify flavonoids from FAM, which are thought to be the major renal protective constituents in FAM. After screening six different kinds of macroporous resins, HPD-100 was selected for its great adsorption and desorption capacity. Then, orthogonal design tests were used to optimize parameters in the processes of impurity removal and flavonoids of FAM desorption on column chromatogram. Moreover, process scale-up was performed, and purification effects maintained after amplification. After purification, the content of seven main flavonoids in the product increased from 8.29% to 51.43%. Protective and anti-inflammatory effects of crude extract and the flavonoid component of FAM after purification were investigated on the adriamycin-damaged HK-2 cells and lipopolysaccharide-stimulated Raw 264.7 cells models. Both bioactivities were improved greatly after purification for these two cell models. Therefore, the purification process had enriched the main bioactive constituents with potential alleviating kidney injury activities. The flavonoid component of FAM is worthy of being developed as an improved remedy for CKD with better patients’ compliance.http://www.mdpi.com/1420-3049/23/10/2649Abelmoschus manihot flowersmacroporous resinsflavonoids |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhenzhong Yang Haitao Tang Qing Shao Anna Rita Bilia Yi Wang Xiaoping Zhao |
spellingShingle |
Zhenzhong Yang Haitao Tang Qing Shao Anna Rita Bilia Yi Wang Xiaoping Zhao Enrichment and Purification of the Bioactive Flavonoids from Flower of Abelmoschus manihot (L.) Medic Using Macroporous Resins Molecules Abelmoschus manihot flowers macroporous resins flavonoids |
author_facet |
Zhenzhong Yang Haitao Tang Qing Shao Anna Rita Bilia Yi Wang Xiaoping Zhao |
author_sort |
Zhenzhong Yang |
title |
Enrichment and Purification of the Bioactive Flavonoids from Flower of Abelmoschus manihot (L.) Medic Using Macroporous Resins |
title_short |
Enrichment and Purification of the Bioactive Flavonoids from Flower of Abelmoschus manihot (L.) Medic Using Macroporous Resins |
title_full |
Enrichment and Purification of the Bioactive Flavonoids from Flower of Abelmoschus manihot (L.) Medic Using Macroporous Resins |
title_fullStr |
Enrichment and Purification of the Bioactive Flavonoids from Flower of Abelmoschus manihot (L.) Medic Using Macroporous Resins |
title_full_unstemmed |
Enrichment and Purification of the Bioactive Flavonoids from Flower of Abelmoschus manihot (L.) Medic Using Macroporous Resins |
title_sort |
enrichment and purification of the bioactive flavonoids from flower of abelmoschus manihot (l.) medic using macroporous resins |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2018-10-01 |
description |
Flower of Abelmoschus manihot (FAM) is clinically effective to treat chronic kidney disease (CKD) with a relatively high dosage. To improve the efficacy and the compliance of patients, macroporous resins were adopted to enrich and purify flavonoids from FAM, which are thought to be the major renal protective constituents in FAM. After screening six different kinds of macroporous resins, HPD-100 was selected for its great adsorption and desorption capacity. Then, orthogonal design tests were used to optimize parameters in the processes of impurity removal and flavonoids of FAM desorption on column chromatogram. Moreover, process scale-up was performed, and purification effects maintained after amplification. After purification, the content of seven main flavonoids in the product increased from 8.29% to 51.43%. Protective and anti-inflammatory effects of crude extract and the flavonoid component of FAM after purification were investigated on the adriamycin-damaged HK-2 cells and lipopolysaccharide-stimulated Raw 264.7 cells models. Both bioactivities were improved greatly after purification for these two cell models. Therefore, the purification process had enriched the main bioactive constituents with potential alleviating kidney injury activities. The flavonoid component of FAM is worthy of being developed as an improved remedy for CKD with better patients’ compliance. |
topic |
Abelmoschus manihot flowers macroporous resins flavonoids |
url |
http://www.mdpi.com/1420-3049/23/10/2649 |
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