Fecal Microbiome and Resistome Profiling of Healthy and Diseased Pakistani Individuals Using Next-Generation Sequencing
In this paper, we aimed to characterize the fecal microbiome and its resistomes of healthy and diseased subjects infected with multidrug-resistant <i>Escherichia coli</i> using next-generation sequencing (NGS). After initial screening, 26 stools samples belonging to healthy (<i>n&l...
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doaj-bc477ad35c5f4d7484f2e7c7b095073e2021-03-18T00:01:21ZengMDPI AGMicroorganisms2076-26072021-03-01961661610.3390/microorganisms9030616Fecal Microbiome and Resistome Profiling of Healthy and Diseased Pakistani Individuals Using Next-Generation SequencingOme Kalsoom Afridi0Johar Ali1Jeong Ho Chang2Department of Biology Education, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu 41566, KoreaCenter for Genome Sciences, Rehman Medical College, Phase-V, Hayatabad, Peshawar 25000, PakistanDepartment of Biology Education, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu 41566, KoreaIn this paper, we aimed to characterize the fecal microbiome and its resistomes of healthy and diseased subjects infected with multidrug-resistant <i>Escherichia coli</i> using next-generation sequencing (NGS). After initial screening, 26 stools samples belonging to healthy (<i>n</i> = 13) and diseased subjects (<i>n</i> = 13) were selected and subjected to NGS. A total of 23 and 42 antibiotic-resistant genes (ARGs) conferring resistance to 6 and 9 classes of antibiotics were identified in the resistomes of healthy and diseased subjects, respectively. <i>Bacteroidetes</i> were found to be the major phylum in both healthy and diseased subjects; however, <i>Proteobacteria</i> was predominantly present in the diseased subjects only. Microbial dysbiosis and predominance of various ARGs in the resistome of diseased subjects reflect the excessive usage of antibiotics in Pakistan and warrants immediate attention to regulate the use of various antimicrobials.https://www.mdpi.com/2076-2607/9/3/616antibioticsgut microbiotanext-generation sequencingmicrobial dysbiosisloss of microbiota diversity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ome Kalsoom Afridi Johar Ali Jeong Ho Chang |
spellingShingle |
Ome Kalsoom Afridi Johar Ali Jeong Ho Chang Fecal Microbiome and Resistome Profiling of Healthy and Diseased Pakistani Individuals Using Next-Generation Sequencing Microorganisms antibiotics gut microbiota next-generation sequencing microbial dysbiosis loss of microbiota diversity |
author_facet |
Ome Kalsoom Afridi Johar Ali Jeong Ho Chang |
author_sort |
Ome Kalsoom Afridi |
title |
Fecal Microbiome and Resistome Profiling of Healthy and Diseased Pakistani Individuals Using Next-Generation Sequencing |
title_short |
Fecal Microbiome and Resistome Profiling of Healthy and Diseased Pakistani Individuals Using Next-Generation Sequencing |
title_full |
Fecal Microbiome and Resistome Profiling of Healthy and Diseased Pakistani Individuals Using Next-Generation Sequencing |
title_fullStr |
Fecal Microbiome and Resistome Profiling of Healthy and Diseased Pakistani Individuals Using Next-Generation Sequencing |
title_full_unstemmed |
Fecal Microbiome and Resistome Profiling of Healthy and Diseased Pakistani Individuals Using Next-Generation Sequencing |
title_sort |
fecal microbiome and resistome profiling of healthy and diseased pakistani individuals using next-generation sequencing |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2021-03-01 |
description |
In this paper, we aimed to characterize the fecal microbiome and its resistomes of healthy and diseased subjects infected with multidrug-resistant <i>Escherichia coli</i> using next-generation sequencing (NGS). After initial screening, 26 stools samples belonging to healthy (<i>n</i> = 13) and diseased subjects (<i>n</i> = 13) were selected and subjected to NGS. A total of 23 and 42 antibiotic-resistant genes (ARGs) conferring resistance to 6 and 9 classes of antibiotics were identified in the resistomes of healthy and diseased subjects, respectively. <i>Bacteroidetes</i> were found to be the major phylum in both healthy and diseased subjects; however, <i>Proteobacteria</i> was predominantly present in the diseased subjects only. Microbial dysbiosis and predominance of various ARGs in the resistome of diseased subjects reflect the excessive usage of antibiotics in Pakistan and warrants immediate attention to regulate the use of various antimicrobials. |
topic |
antibiotics gut microbiota next-generation sequencing microbial dysbiosis loss of microbiota diversity |
url |
https://www.mdpi.com/2076-2607/9/3/616 |
work_keys_str_mv |
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